Abstract
A total of three Gram-positive, and oxidase and catalase-negative facultative anaerobic non-motile bacteria were isolated from the rumen fluid of cows and goats and these strains were designated CNU_G2T, CNU_77-61, and CNU_G3. They grew at 20–45 °C, pH 6.5–7, and 0–6.5% NaCl (w/v). The G + C contents (%) of the three isolates were 37.9, 37.8 and 37.8, respectively. Phylogenomic analysis indicated that these strains were distinct from other Streptococcus species. The average nucleotide identity between the isolates and the closest strain S. infantarius subsp. infantarius ATCC BAA-102T was 94.0–94.5%, while the digital DNA–DNA hybridization (dDDH) values between the isolates and the aforementioned related strain were 58.2–61.4%, respectively. Fatty acid analysis revealed higher proportions of C16:0 (> 28%) in all three isolates, while the proportion of C18:0 was higher in CNU_G2T (25.8%); however, it was less than 12% in all the representing strains used in the study. The C14:0 composition of strains CNU_77-61 (22.1%) and CNU_G3 (24.1%) was higher than that of type strains of CNU_G2T (8.1%). Based on the morphological, biochemical, and molecular phylogenetic features of the three novel isolates, they represent a novel species of the genus Streptococcus, for which we propose as Streptococcus ruminicola sp. nov. The type strain is CNU_G2T (= KCTC 43308T = GDMCC 1.2785T).
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Abbreviations
- SBSEC:
-
Streptococcus bovis/Strerptococcus equinus Complex
- BHIA:
-
Brain heart infusion agar
- ANI:
-
Average nucleotide identity
- GGDC:
-
Genome-to-genome distance
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Funding
This work was supported by the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program (KGM5232221), the National Research Foundation of Korea (NRF-2020R1I1A2068827), and the Development of technology for biomaterialization of marine fisheries byproducts of Korea Institute of Marine Science & Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (KIMST-20220128).
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Conceptualization, SYP, SS and JHK; data curation, SYP, NM and HK; formal analysis, SYP, NM and HK; funding acquisition, SGK. and JHK; investigation, SYP and SGK; methodology, SYP, NM, and HK; project administration, JHK; resources, SYP and SS; supervision, SS and JHK; writing-original draft, SYP, NM, and JHK; and writing-review and editing, SYP, SGK and JHK All authors have read and agreed to the published version of the manuscript.
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This study was conducted at the Center for Animal Science Research, Chungnam National University, Korea. The animal use and protocols for this experiment were reviewed and approved by the Chungnam National University Animal Research Ethics Committee (IACUC approval Nos. CNU-00455 (April, 2014) and CNU-01021 (April, 2018)).
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Park, S.Y., Muhammad, N., Kim, SG. et al. Streptococcus ruminicola sp. nov., new species of the Streptococcus bovis/Streptococcus equinus complex (SBSEC) isolated from the rumen of Korean domestic ruminants. Arch Microbiol 204, 636 (2022). https://doi.org/10.1007/s00203-022-03255-4
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DOI: https://doi.org/10.1007/s00203-022-03255-4