Abstract
A Gram-strain-negative, rod-shaped, aerobic bacterium, designated strain TRM 85114T, was isolated from the Jincaotan wetland in the Pamir Plateau of China. This strain grew optimally at 30 °C and pH 6.0 in the presence of 3% (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain TRM 85114T was affiliated with the genus Halomonas, and shared high sequence similarity with Halomonas korlensis XK1T (97.3%) and Halomonas tibetensis pyc13T (96.4%). Strain TRM 85114T contained C16:0 and C19:0 cyclo ω8c as primary cellular fatty acids, Q-9 as predominate respiratory quinone, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids of unknown structure containing glucosamine, unidentified aminophospholipids, unidentified lipids and three unidentified phospholipids as the major polar lipids. The complete genome of TRM 85114T comprised 3,902 putative genes with a total of 4,126,476 bp and a G + C content of 61.6%. The average nucleotide identity and digital DNA–DNA hybridization values between strain TRM 85114T and related type Halomonas strains of H. korlensis XK1T, H. tibetensis pyc13T, Chromohalobacter salexigens DSM 6768T, and Halomonas urumqiensis BZ-SZ-XJ27T were 75.4–88.9% and 22.9–39.2%, respectively. Based on phenotypic, chemotaxonomic, and molecular features, strain TRM 85114T represents a novel species of the genus Halomonas, for which the name is proposed as Halomonas jincaotanensis sp. nov.. The type strain is TRM 85114T (CCTCC AB 2021006T = LMG 32311T). The amount of 1-naphthylamine degradation by strain TRM 85114T reached up to 32.0 mg/L in 14 days.
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Acknowledgements
This study was supported by the National Natural Science Foundation of China (31900007), Bingtuan Science and Technology Program (2018BC008), the Microbial Resources Utilization Innovation Team in Key Field of Xin Jiang Production and Construction Corps (2017CB014), and the principal fund (TDHNLH201604).
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This study was supported by the National Natural Science Foundation of China (31900007), Bingtuan Science and Technology Program (2018BC008), the Microbial Resources Utilization Innovation Team in Key Field of Xin Jiang Production and Construction Corps (2017CB014), and the principal fund (TDHNLH201604).
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XB performed the experiments, analyzed the data, and drafted the manuscript. ZL conducted the degradation test of 1-naphthylamine. ZX, CW, and MR critically revised the manuscript. LZ contributed to the creation. All authors read and approved the manuscript. We thank HC and PX for finding and providing Halomonas tibetensis pyc13T.
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Bu, X., Xia, Z., Liu, Z. et al. Halomonas jincaotanensis sp. nov., isolated from the Pamir Plateau degrading polycyclic aromatic hydrocarbon. Arch Microbiol 204, 398 (2022). https://doi.org/10.1007/s00203-022-03008-3
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DOI: https://doi.org/10.1007/s00203-022-03008-3