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Multidrug-resistant epi-endophytic bacterial community in Posidonia oceanica of Mahdia coast as biomonitoring factor for antibiotic contamination

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Abstract

Faced with the significant disturbances, mainly of anthropogenic origin, which affect the Mediterranean coastal ecosystem, Posidonia oceanica (L.) Delile has often been used to assess the state of health of this environment. The present study aims to determine the multidrug resistance patterns among isolated and identified epi-endophytic bacterial strains in P. oceanica seagrass collected from Mahdia coastal seawater (Tunisia). To investigate the bacterial community structure and diversity from coastal seawater samples from Mahdia, total DNA extraction and 16S rRNA gene amplification were performed and analyzed by denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed that some bands were specific to a given site, while other bands were found to be common to more than one sample. In the other hand, bacterial strains were isolated from 1 mL of leaves and epiphytes suspension of P. oceanica seagrass in marine agar. Forty-three isolates were obtained, seven of them were selected and identified on the basis of 16S rRNA gene sequence analysis. These isolates belonged to the genus Bacillus, exhibiting 98–100% of identity with known sequences. Susceptibility patterns of these strains were studied toward commonly used antibiotics in Tunisia. All identified isolates were resistant to Aztreonam (72.1%), Ceftazidime (60.5%), Amoxicillin (56%) and Rifampicin (51.2%). S5-L13 strain had presented the highest multidrug resistance with a MAR index of 0.67.

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Acknowledgements

This work was supported by the UR Analysis and Process Applied to the Environment APAE (Mahdia, Tunisia) and by the Tunisian Ministry of Higher Education and Scientific Research

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  1. Amel Jebara and Wafa Hassen have contributed equally.

Authors and Affiliations

  1. Research Unit of Analysis and Process Applied on the Environment UR17ES32 ISSAT- 5121 Mahdia, University of Monastir, Monastir, Tunisia

    Amel Jebara, Wafa Hassen, Amira Ousleti, Lotfi Mabrouk, Ahlem Jaziri & Hedi Ben Mansour

  2. BioMorf Department, University of Messina, Polo SS Annunziata, 98168, Messina, Italy

    Giuseppa Di Bella

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  1. Amel Jebara
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Contributions

WH and HBM conceived and designed the experiments. AJ and WH carried out the functional experiments, analyzed the data and wrote the manuscript. WH, LM, GDB and HBM revised the manuscript.

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Correspondence to Hedi Ben Mansour.

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203_2022_2842_MOESM1_ESM.doc

Supplementary file1 (DOC 1879 kb) Fig. 1 Sampling site location of the Seawater and P. oceanica seagrass (S1: 35°50’57.86’’ N, 11°07’10.20’’ E; S2: 35°47’20.46” N, 11°05’37.14” E; S3: 35°39’04.91” N, 11°04’97.51” E; S4: 35°22’94.77” N, 11°16’24.38” E; S5: 35°15’97.97” N, 11°08’64.41” E). Fig. 2 Denaturing gradient gel electrophoresis (DGGE) gel profiles from triplicate of five seawater samples showing the bacterial community structure based on the variable region V3–V5 of 16S rRNA. Urea and formamide gradient ranged from 40% to 60%. Three kinds of bands were distinct with correlation to the running level, as short (A), medium (B) and long migration bands (C). Species richness index (R) and Simpson diversity index (D) were indicated in the lower part of the gel

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Jebara, A., Hassen, W., Ousleti, A. et al. Multidrug-resistant epi-endophytic bacterial community in Posidonia oceanica of Mahdia coast as biomonitoring factor for antibiotic contamination. Arch Microbiol 204, 229 (2022). https://doi.org/10.1007/s00203-022-02842-9

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