Abstract
Class D β-lactamases OXA-232 and OXA-48 hydrolyze penicillin, cephalosporins and carbapenems, limiting the pharmacological therapeutics in bacteraemia. OXA producer microorganisms are considered a great emergent threat, especially in nosocomial environments. To determine the resistance profile and genomic characterization of two isolates initially identified as potential carbapenemase-producer Klebsiella oxytoca in a third level hospital. Automated platform BD Phoenix-100 System was used to identify and to biochemically characterize both isolates. Furthermore, the resistance profile was determined through CLSI methods and the whole genome sequences were obtained using Next-Generation Sequencing. Resistance genes were analyzed, and the virtual fingerprinting was determined to corroborate the similarity with related bacteria. Both strains correspond to Raoultella ornithinolytica carrying OXA 232 and OXA-48 genes, confirming the class D β-lactamases assay results. Here, we present the genetic and phenotypic analysis of multidrug resistance R. ornithinolytica, representing the first report in Mexico.
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IAC-O original idea, samples collection and data analysis, FG-A NGS sequence and bioinformatic analysis, AAR NGS sequence and bioinformatic analysis. JER-G critical review and discussion of data. NE-E critical review and discussion of data. JCJ-G bacteriological tests. JCB-A antimicrobial tests. VHG-M phenotypical analysis. JMB-L macro array test and discussion results. EMC article writing, data analysis, and organization of results.
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Cortés-Ortíz, I.A., Mendieta-Condado, E., Escobar-Escamilla, N. et al. Multidrug-resistant Raoultella ornithinolytica misidentified as Klebsiella oxytoca carrying blaOXA β-lactamases: antimicrobial profile and genomic characterization. Arch Microbiol 203, 5755–5761 (2021). https://doi.org/10.1007/s00203-021-02515-z
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DOI: https://doi.org/10.1007/s00203-021-02515-z