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Diesel degradation efficiency of Enterobacter sp., Acinetobacter sp., and Cedecea sp. isolated from petroleum waste dumping site: a bioremediation view point

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Abstract

Bioremediation through biodegradation is applied for cleaning up several environmental pollutions including petroleum oil spill containing petrol, diesel, mobil, kerosene, lubricating, etc. which have devastated several endangered terrestrial and aquatic ecosystems. Therefore, the current research was aimed to isolate and identify diesel degrading bacteria from the petroleum waste dumping site and determined their degrading efficiency. The bacterial strains were isolated through a minimum salt medium supplemented with 2% diesel as the sole carbon source. The bacteria were identified by morphological, biochemical characterization, and 16S rRNA gene sequencing. The optimized growth pattern was evaluated by utilization of a wide range of temperatures (25, 30, 35, and 40 °C) and pH (5,6,7 and 8) as well as different concentrations of diesel (2, 3, 5and 7%). Finally, the degradation rate was determined by measuring the residual diesel after 7, 14, and 21 days of incubation. The study isolated Enterobacter ludwigii, Enterobacter mori, Acinetobacter baumannii, and Cedecea davisae where all are gram-negative rod-shaped bacilli. All the bacterial strains utilized the diesel at their best at 30 °C and pH 7, among them, A. baumannii and C. davisae exhibited the best degrading efficiency at all applied concentrations. Finally, the determination of degradation rate (%) through gravimetrical analysis has confirmed the potency of A. Baumannii and C. davisae where the degradation rate was around 61 and 52% respectively after 21 days of incubation period with 10% diesel. The study concludes that all of those isolated bacterial consortiums, especially A. baumannii and C. davisae could be allocated as active agents used for bioremediation to detoxify the diesel-containing contaminated sites in a cost-effective and eco-friendly way.

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Data availability

All the data expressed in the present paper will be readily available from me upon request (reza.gen@ru.ac.bd).

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Acknowledgements

All the authors would like to truthfully acknowledge the Institute of Biological Science (IBSc), and Central Laboratory, Department of Botany, University of Rajshahi, Bangladesh for providing the bacterial culture facilities. Again, The heartfelt gratitude from the author to Baneshwar Filling Station, Rajshahi for providing the soil sample.

Funding

This research did not receive any specific grant from funding agencies. This research work was carried out based on the internal fund of Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh.

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Authors and Affiliations

  1. Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, Rajshahi University, Rajshahi, 6205, Bangladesh

    Israt Jerin, Md. Sifat Rahi, Tanjia Sultan, Md. Shihabul Islam, Salek Ahmed Sajib, Kazi Md. Faisal Hoque & Md Abu Reza

  2. Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh

    Md. Sifat Rahi

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  1. Israt Jerin
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  2. Md. Sifat Rahi
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  3. Tanjia Sultan
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  4. Md. Shihabul Islam
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  5. Salek Ahmed Sajib
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  6. Kazi Md. Faisal Hoque
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  7. Md Abu Reza
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Contributions

The conception of the study and experimental design carried out by IJ and MAR. Where IJ, TS, SAS were performed most of the experiments. MSR, IJ analyzed the results and prepared the manuscript. MSI has a contribution to statistical analysis. MAR and KMFH were involved in intellectual contribution and manuscript editing.

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Correspondence to Md Abu Reza.

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Communicated by Erko Stackebrandt.

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Jerin, I., Rahi, M.S., Sultan, T. et al. Diesel degradation efficiency of Enterobacter sp., Acinetobacter sp., and Cedecea sp. isolated from petroleum waste dumping site: a bioremediation view point. Arch Microbiol 203, 5075–5084 (2021). https://doi.org/10.1007/s00203-021-02469-2

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  • DOI: https://doi.org/10.1007/s00203-021-02469-2

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