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Cohnella pontilimi sp. nov., isolated from tidal-flat mud

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Abstract

A Gram-positive, aerobic, endospore-forming, rod-shaped bacterial strain, CAU 1483 T, was isolated from tidal-flat mud in the Republic of Korea. It grew optimally at 30 °C, in a pH 7.0 medium with 2% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain CAU 1483 T formed a separate clade within Paenibacillaceae together with members of the genus Cohnella. Strain CAU 1483 T exhibited the highest 16S rRNA gene sequence similarity (97.1%) to C. candidum 18JY8-7 T. Whole genome of strain CAU 1483 T was 4.29 Mb in size with a 53.7 mol% G + C content, and included 4046 coding sequences and included 4046 coding sequences, some of which associated with stress response. The average nucleotide identity and digital DNA–DNA hybridization similarity between strain CAU 1483 T and related members of the genus Cohnella were 71.8–74.9% and 22.6–33.9%, respectively. The major respiratory quinone present in this strain was menaquinone-7. Strain CAU 1483 T contained anteiso-C15:0 and iso-C16:0 as the major fatty acids, while its polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, lysyl-phosphatidylglycerol, phosphatidylcholine, three unidentified aminophospholipids, two unidentified lipids and an unidentified phospholipid. Peptidoglycan type was A1γ meso-Dpm. On the basis of taxonomic characterization, strain CAU 1483 T constitutes a novel species, for which the name Cohnella pontilimi sp. nov. is proposed. The type strain of this novel species is CAU 1483 T (= KCTC 43047 T = NBRC 113953 T).

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Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201902203) and the Chung-Ang University Research Grants in 2019.

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Authors and Affiliations

  1. Department of Microbiology, Chung-Ang University College of Medicine, Seoul, 06974, Republic of Korea

    Jihye Baek, Jong-Hwa Kim & Wonyong Kim

  2. Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea

    Jung-Hoon Yoon

  3. Institute of Bioscience and Biotechnology, Korean Collection for Type Cultures, Jeongeup, Republic of Korea

    Jung-Sook Lee

  4. Department of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla, 90112, Thailand

    Ampaitip Sukhoom

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  1. Jihye Baek
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Baek, J., Kim, JH., Yoon, JH. et al. Cohnella pontilimi sp. nov., isolated from tidal-flat mud. Arch Microbiol 203, 2445–2451 (2021). https://doi.org/10.1007/s00203-021-02266-x

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