Abstract
The aim of this study was to evaluate the phytochemical constituents, antioxidant, antifungal, and anti-virulence activities of traditionally used Mezoneuron benthamianum leaves. Extracts were prepared using acetone and methanol, and the preliminary phytochemical screening was performed. The antioxidant activity was studied using the DPPH method. Anti-Candida albicans activity was established and the effect on the germ tube and phospholipase production, as well as on the host cell adherence was assessed. The extracts showed the presence of anthraquinones, cardiac glycosides, flavonoids, reducing sugars, saponins, steroids, tannins, and terpenoids. Gallic acid and trans-resveratrol were among the predominant phytochemicals found in M. benthamianum. The crude extracts presented significantly higher antioxidant activity than the ascorbic acid standard. At 0.39 mg/mL, acetone extract inhibited the growth of Candida albicans. At lower concentrations (200–50 µg/mL), it significantly inhibited the adherence ability (up to 51%), formation of hyphae (up to 65%), and the production of phospholipase. In conclusion, at high concentrations, M. benthamianum kills C. albicans, and at lower concentrations, it can inhibit the virulence properties of this pathogen. This study on crude extract validates the traditional use of this plant. However, further research is required to establish the anti-virulence activity of the two compounds and their therapeutic potential.
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OPM and OOO appreciate the Principal Technologist, Mrs A.E. Ayano, and the entire staff of the Central Teaching Laboratory of Bells University of Technology for the permission to use their facilities.
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NS performed some of the laboratory experiments and data analysis, GZ, OOO, and IKC performed some of the laboratory experiments, OPM and PM conceptualized the research project, coordinated the investigations, and prepared manuscript.
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Communicated by Erko Stackebrandt.
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Nciki, S., Oderinlo, O.O., Gulube, Z. et al. Mezoneuron benthamianum inhibits cell adherence, hyphae formation, and phospholipase production in Candida albicans. Arch Microbiol 202, 2533–2542 (2020). https://doi.org/10.1007/s00203-020-01972-2
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DOI: https://doi.org/10.1007/s00203-020-01972-2