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Lysobacter gilvus sp. nov., isolated from activated sludge

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Abstract

Strain HX-5-24T was isolated from the sludge collected from the outlet of the biochemical treatment facility of an agricultural chemical plant in Maanshan city, Anhui province, PR China (118° 28′ N, 31° 47′ E). Cells were observed to be Gram-reaction-negative, rod-shaped, non-motile and aerobic. Strain HX-5-24T shared 99.1% 16S rRNA gene sequence similarity with Lysobacter dokdonensis DS-58T and less than 97% similarities with other type strains. The phylogenetic analysis based on 16S rRNA indicated that strain HX-5-24T belonged to the genus Lysobacter and formed a subclade with L. dokdonensis DS-58T. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain HX-5-24T and L. dokdonensis DS-58T were 87.5% and 35.3%, respectively. The genomic DNA G + C content of the strain was 66.4%. The major fatty acids (> 5%) were iso-C15:0, anteiso-C15:0, iso-C16:0, C16:0 and summed feature 9 (iso-C17:1 ω9c and/or C16:0 10-methyl). The predominant quinone was ubiquinone Q-8. The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and phospholipids (PL). On the basis of phenotypic and phylogenetic evidences, strain HX-5-24T is considered as a novel species in the genus Lysobacter, for which the name Lysobacter gilvus sp. nov. is proposed. The type strain is HX-5-24T (= KCTC 72470T = CCTCC AB 2019228T).

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Acknowledgements

This work was supported by the National Key R&D Program of China (2016YFD0801102), the National Natural Science Foundation of China (No. 31770117 and 31970096).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Jianyi Xu, Mengyao Sheng, Zhou Yang, Jiguo Qiu & Jian He

  2. The College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Jun Zhang

  3. Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, 637099, Sichuan, China

    Lei Zhang

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  1. Jianyi Xu
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  2. Mengyao Sheng
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  3. Zhou Yang
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  4. Jiguo Qiu
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  5. Jun Zhang
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  7. Jian He
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Correspondence to Jun Zhang or Jian He.

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Communicated by Erko Stackebrandt.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences and the whole genome of strains HX-5-24T are MN786796 and WOXT00000000, respectively.

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Xu, J., Sheng, M., Yang, Z. et al. Lysobacter gilvus sp. nov., isolated from activated sludge. Arch Microbiol 203, 7–11 (2021). https://doi.org/10.1007/s00203-020-01943-7

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