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Profiling of gene expression in methicillin-resistant Staphylococcus aureus in response to cyclo-(l-Val-l-Pro) and chloramphenicol isolated from Streptomyces sp., SUK 25 reveals gene downregulation in multiple biological targets

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Abstract

Chloramphenicol (CAP) and cyclo-(l-Val-l-Pro) were previously isolated from Streptomyces sp., SUK 25 which exhibited a high potency against methicillin-resistant Staphylococcus aureus (MRSA). This study aimed to profile gene expression of MRSA treated with CAP and cyclo-(l-Val-l-Pro) compounds using DNA microarray. Treatment of MRSA with CAP resulted in upregulation of genes involved in protein synthesis, suggesting the coping mechanism of MRSA due to the inhibition of protein synthesis effect from CAP. Most upregulated genes in cyclo-(l-Val-l-Pro) were putative genes with unknown functions. Interestingly, genes encoding ribosomal proteins, cell membrane synthesis, DNA metabolism, citric acid cycle and virulence were downregulated in MRSA treated with cyclo-(l-Val-l-Pro) compound, suggesting the efficacy of this compound in targeting multiple biological pathways. Contrary to CAP, with only a single target, cyclo-(l-Val-l-Pro) isolated from this study had multiple antimicrobial targets that can delay antibiotic resistance and hence is a potential antimicrobial agent of MRSA.

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Acknowledgements

This work was supported by the Ministry of Higher Education (MOHE) under Grant (FRGS/1/2011/ST/UKM/02/1); and Universiti Kebangsaan Malaysia under Grant (MI-2018-004).

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Authors and Affiliations

  1. Programme of Biomedical Science, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia

    Noraziah M. Zin, Muhanna M. Al-shaibani & Asif Sukri

  2. Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia

    Juriyati Jalil

  3. Medical Microbiology Department, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Anis Rageh Al-Maleki

  4. Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Sana’a University, Sana’a, Yemen

    Anis Rageh Al-Maleki

  5. Faculty of Agro-Based Industry, Universiti Malaysia Kelantan, 17600, Jeli, Kelantan, Malaysia

    Nik Marzuki Sidik

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Zin, N.M., Al-shaibani, M.M., Jalil, J. et al. Profiling of gene expression in methicillin-resistant Staphylococcus aureus in response to cyclo-(l-Val-l-Pro) and chloramphenicol isolated from Streptomyces sp., SUK 25 reveals gene downregulation in multiple biological targets. Arch Microbiol 202, 2083–2092 (2020). https://doi.org/10.1007/s00203-020-01896-x

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