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Adhesion, anti-adhesion and aggregation properties relating to surface charges of selected Lactobacillus strains: study in Caco-2 and H357 cells

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Abstract

This study aimed to assess adhesion and anti-adhesion, aggregation, and surface properties of four selected oral Lactobacillus strains, L. fermentum SD7, L. paracasei SD1, L. rhamnosus SD4, and L. rhamnosus SD11, together with Lactobacillus rhamnosus GG. Human cells, enterocytes Caco-2 and oral keratinocyte H357 were used, and various enteric and oral pathogens were included. Results showed that all Lactobacillus tested gave high adhesion and internalization in both Caco-2 and H357 cells similar to L. rhamnosus GG, and it suggests that such properties are strain dependent and specific to host cells. Anti-adhesion was different; it depended on the internalization ability of individual Lactobacillus and pathogenic strains to Caco-2 and H357. Coaggregation ability depended on autoaggregation of both the Lactobacillus and pathogenic strains. A positive correlation between surface charges and aggregation, and internalization and anti-adhesion of all Lactobacillus was found. In conclusion, results suggests that the selected Lactobacillus might be potential probiotics for usage in both the oral cavity and intestinal tract due to their abilities of aggregation, adherence and anti-internalization to both Caco-2 and H357 cells.

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Acknowledgements

This study was supported by the National Research Council of Thailand (DEN600045b), the Thailand Research Fund (TRF) through the Royal Golden Jubilee PhD program (PhD/0217/2553/AN.2), and the National Science and Technology Development Agency (Co-project, 2017).

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Authors and Affiliations

  1. Faculty of Dentistry, Common Oral Diseases and Epidemiology Research Center, Prince of Songkla University, Hat-Yai, Thailand

    Benchamat Sophatha, Supatcharin Piwat & Rawee Teanpaisan

  2. Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Thailand

    Benchamat Sophatha & Rawee Teanpaisan

  3. Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Thailand

    Supatcharin Piwat

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  1. Benchamat Sophatha
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  2. Supatcharin Piwat
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  3. Rawee Teanpaisan
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Correspondence to Rawee Teanpaisan.

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Communicated by Erko Stackebrandt.

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Sophatha, B., Piwat, S. & Teanpaisan, R. Adhesion, anti-adhesion and aggregation properties relating to surface charges of selected Lactobacillus strains: study in Caco-2 and H357 cells. Arch Microbiol 202, 1349–1357 (2020). https://doi.org/10.1007/s00203-020-01846-7

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  • DOI: https://doi.org/10.1007/s00203-020-01846-7

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