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Algicoccus marinus gen. nov. sp. nov., a marine bacterium isolated from the surface of brown seaweed Laminaria japonica

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Abstract

A Gram-staining-negative, strictly aerobic, non-motile, ovoid- to rod-shaped bacterium, designated as HZ20T, was isolated from the surface of a brown seaweed (Laminaria japonica) sample collected from the East China Sea. Colonies are 1.0–2.0 mm in diameter, smooth, circular, convex and yellow after grown on MA at 28 °C for 72 h. The strain was found to grow at 4–50 °C (optimum, 37 °C), pH 5.0–9.5 (optimum, pH 7.0–7.5) and with 0–10% (w/v) NaCl (optimum, 1.0–1.5%). Chemotaxonomic analysis showed ubiquinone-8 as the only quinone, C17:0 cyclo, C16:0, summed feature 8 (C18:1ω7c and/or C18:1ω6c) and summed feature 2 (C12:0 aldehyde/unknown 10.9525/C16:1 iso I/C14:0 3OH) as the major fatty acids (> 5%), and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified amino phospholipid, two unidentified phospholipids, five unidentified glycolipid and two unidentified lipids as the polar lipids. The DNA G + C content was 55.5 mol %. 16S rRNA gene sequences of the isolate showed highest similarities to Bordetella flabilis AU10664T (97.1%), Parapusillimonas granuli Ch07T (97.1%), Paracandidimonas soli IMT-305T (97.1%), Kerstersia gyiorum LMG5906T (97.0%) and Bordetella sputigena LMG 28641T (97.0%). The phylogenetic trees using 16S rRNA gene and genome sequences both showed that the strain HZ20T formed a deep branch separated from other related genera, indicating that it represents a novel species of a novel genus. The calculated average nucleotide identity (ANI) and percent of conserved proteins (POCP) values using genome sequences of strain HZ20T and related strains also support this conclusion. Based on the phenotypic properties and phylogenetic distinctiveness, we propose strain HZ20T (= MCCC 1K03465T = KCTC 62330T) to represent a novel species of a novel genus with the name Algicoccus marinus gen. nov. sp. nov.

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Abbreviations

POCP:

Percent of conserved proteins

ANI:

Average nucleotide identity

DPG:

Diphosphatidylglycerol

PG:

Phosphatidylglycerol

PE:

Phosphatidylethanolamine

PS:

Phosphatidylserine

APL:

Unidentified aminophospholipid

PL:

Unidentified phospholipid

AL:

Unidentified aminolipid

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Acknowledgements

This work is supported by the Zhejiang Provincial Natural Science Foundation (LQ19C010005) and the Science Foundation of Zhejiang Sci-Tech University (16042186-Y).

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Authors and Affiliations

  1. Lab of Marine Functional Molecules, 928th Second Avenue, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, People’s Republic of China

    Jun-Jie Ying, Shun-Li Zhang, Chao-Yu Huang, Lin Xu & Cong Sun

  2. College of Life Sciences, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Zhe Zhao & Min Wu

  3. Ocean College, Zhejiang University, Zhoushan, 316000, People’s Republic of China

    Min Wu

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  1. Jun-Jie Ying
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Correspondence to Cong Sun.

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Communicated by Erko Stackebrandt.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences and complete genome sequences of strain HZ20T are MG712864 and CP028901, respectively. The DPD number of strain HZ20T is TA00577.

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Ying, JJ., Zhang, SL., Huang, CY. et al. Algicoccus marinus gen. nov. sp. nov., a marine bacterium isolated from the surface of brown seaweed Laminaria japonica. Arch Microbiol 201, 943–950 (2019). https://doi.org/10.1007/s00203-019-01664-6

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