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Roseovarius lacus sp. nov., isolated from Yuncheng Saline Lake, China

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Abstract

Strain GSS12T, a Gram-negative, aerobic, non-flagellated, ovoid- to rod-shaped (0.5–0.7 × 0.9–3.0 µm) bacterium, was isolated from Yuncheng Saline Lake, China. Growth occurred with 0.5–16.0 % (w/v) NaCl (optimum 4.5 %), at pH 5.0–10.0 (optimum pH 6.0–6.5) and at 10–50 °C (optimum 37 °C). The major fatty acids (>5.0 %) found in GSS12T were summed feature 8 (72.2 %), C16:0 (9.0 %) and C18:1 ω7c 11-methyl (6.4 %). The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequences showed that strain GSS12T forms a stable clade with species of the genus Roseovarius, being related to R. pacificus 81-2T and R. litoreus GSW-M15T with 97.9 and 96.7 % of sequence similarity, respectively. The DNA–DNA relatedness values between strain GSS12T and R. pacificus 81-2T and R. halotolerans HJ50T were low (36 and 29 %, respectively). The phenotypic, physiological, biochemical and genetic characteristics support the assignment of strain GSS12T to the genus Roseovarius and represent a novel species. The name Roseovarius lacus sp. nov. is proposed, with strain GSS12T (=KCTC 52185T =MCCC 1K02302T) as the type strain.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (41501546), the Science and Technology Planning Project of Guangdong Province, China (2016A020221020 and 2013B030700004), and the Science and Technology Planning Project of Guangzhou City, China (201508020010 and 2014Y2-00522).

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Authors and Affiliations

  1. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, People’s Republic of China

    Jiafeng Pan & Zhi Liu

  2. Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People’s Republic of China

    Jiafeng Pan, Zhen Yu, Jia Tang, Guiqin Yang & Li Zhuang

  3. College of Resources and Environmental Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, People’s Republic of China

    Shungui Zhou

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  1. Jiafeng Pan
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  2. Zhen Yu
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  3. Jia Tang
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  4. Guiqin Yang
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  5. Li Zhuang
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  6. Zhi Liu
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  7. Shungui Zhou
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Corresponding author

Correspondence to Zhen Yu.

Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequences of strain GSS12T is KX417301.

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Pan, J., Yu, Z., Tang, J. et al. Roseovarius lacus sp. nov., isolated from Yuncheng Saline Lake, China. Arch Microbiol 199, 231–236 (2017). https://doi.org/10.1007/s00203-016-1296-3

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  • DOI: https://doi.org/10.1007/s00203-016-1296-3

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