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Morphogenesis and alpha-tubulin gene of plasmodium in Didymium megalosporum

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Abstract

The morphogenesis of plasmodium in Didymium megalosporum was observed for the first time by hanging drop culture and 3 % oat-agar culture under controlled conditions. The development of plasmodium was characteristic formation process of phaneroplasmodium. The mature plasmodium was white yellow or yellow green in color, and had an extending fan-like sheet at the front, followed by a network of veins. It could be easy to fuse into a bigger plasmodium during the formation and die at high temperature or starvation. In view of the important role of alpha-tubulin during the morphogenesis of plasmodium, we sequenced partial sequence of alpha-tubulin gene, a total length of 1,159 bp, in this plasmodium. It had an intron area from 177 to 235 bp, and the exon area had a similarity of 91 % relative to altA locus of alpha-tubulin gene in Physarum polycephalum, the translated amino acid sequence was identical (100 % match) between the two.

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Acknowledgments

This work was supported by National Nature Science Foundation (Project Nos. 31070012) and National Science Technology Support Plan Project (No. 2012BAC01B04).

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Authors and Affiliations

  1. Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, People’s Republic of China

    Xiaoxia Song, Huacheng Zhong, Qi Wang & Yu Li

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  1. Xiaoxia Song
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  2. Huacheng Zhong
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  3. Qi Wang
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  4. Yu Li
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Corresponding author

Correspondence to Qi Wang.

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Communicated by Erko Stackebrandt.

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Song, X., Zhong, H., Wang, Q. et al. Morphogenesis and alpha-tubulin gene of plasmodium in Didymium megalosporum . Arch Microbiol 196, 369–374 (2014). https://doi.org/10.1007/s00203-014-0971-5

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