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Polymorphism rs1800255 from COL3A1 gene and the risk for pelvic organ prolapse

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Abstract

Introduction and hypothesis

Genetic variations of type III collagen may compromise the supportive structures of the female pelvic floor and consequently favor pelvic organ prolapse. The single nucleotide polymorphism G/A rs1800255 located in the coding region for type III collagen (COL3A1) was evaluated as a risk factor for pelvic organ prolapse.

Methods

A single-center prospective cohort study including women with clinical diagnosis of stage III and IV prolapse (POP group) and prolapse stage 0 or I (control group). Sociodemographic, clinical data and obstetric history were retrieved by physician interview. DNA including the rs1800255 polymorphism was amplified by polymerase chain reaction from blood genomic cells and digested with AluI restriction enzyme for distinction of G and A variants. Qualitative variables were compared using the chi-square and Fisher’s exact tests and unpaired t-test for quantitative variables. After stratification of the groups, risk factors for POP were estimated using odds ratios (ORs) from the binary logistic regression model.

Results

A total of 292 women were included, 112 in the POP group and 180 in the control group. There was no significant difference between groups regarding rs1800255. Age and home birth were the only significant risk factors for pelvic organ prolapse.

Conclusion

Polymorphism rs1800255 from COL3A1 gene was not a risk factor for pelvic organ prolapse.

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Acknowledgments

We thank the colleagues in FMABC from the Department of Obstetrics and Gynecology for helping to recruit participants and from the Molecular Biology Laboratory for helping in genetic analysis.

Funding

The work was funded by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) grant #2014/01107–6 and NEPAS (Núcleo de Estudos, Pesquisa e Assessoria à Saúde). Fernando Henrique Teixeira received a fellowship from PIBIC-CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico).

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Authors and Affiliations

  1. Faculdade de Medicina do ABC, Av. Lauro Gomes, 2000, Santo André, SP, CEP 09060-870, Brazil

    Fernando Henrique Teixeira, César Eduardo Fernandes, Ricardo Peres do Souto & Emerson de Oliveira

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  1. Fernando Henrique Teixeira
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  2. César Eduardo Fernandes
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  3. Ricardo Peres do Souto
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  4. Emerson de Oliveira
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Correspondence to Ricardo Peres do Souto.

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Teixeira, F.H., Fernandes, C.E., do Souto, R.P. et al. Polymorphism rs1800255 from COL3A1 gene and the risk for pelvic organ prolapse. Int Urogynecol J 31, 73–78 (2020). https://doi.org/10.1007/s00192-019-03965-2

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  • DOI: https://doi.org/10.1007/s00192-019-03965-2

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