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Bladder wall injection of mesenchymal stem cells ameliorates bladder inflammation, overactivity, and nociception in a chemically induced interstitial cystitis-like rat model

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A Commentary to this article was published on 29 March 2018

Abstract

Introduction and hypothesis

We investigated the effects of bladder wall injection of mesenchymal stem cells (MSCs) on bladder tissues, function, and nociceptive behavior in a chemically induced interstitial cystitis-like rat model.

Methods

Chemical cystitis of female rats was induced by intravesical instillation of 0.1 N hydrochloride (HCl) once a week for 2 weeks. Bladders were harvested 1, 2, 3, and 4 weeks after the second application for histological examination. Adipose-derived MSCs (HCl + MSCs) or phosphate-buffered saline (HCl + PBS) was injected into the bladder wall at the time of the second application of HCl. Histological examination, nociceptive behavior, and cystometrograms were evaluated 2 weeks after the injection compared with controls, which received instillation and injection of PBS into the bladder (sham + PBS).

Results

The number of mast cells and expression of tumor necrosis factor-α (TNF-α) and transforming growth factor-β (TGF-β) were significantly increased at 1 and 2 weeks, and expression of collagen fibers was significantly increased from 2–4 weeks after the second application of HCl. Significantly increased nociceptive behavior, number of mast cells, expression of TNF-α, TGF-β, and collagen fibers were observed in HCl + PBS compared with sham + PBS, whereas these changes were significantly decreased in HCl + MSCs compared with HCl + PBS. In addition, bladder capacity and voiding threshold pressures were significantly decreased in HCl + PBS but not in HCl + MSCs compared with sham + PBS.

Conclusions

The results suggest that bladder injection of MSCs ameliorates inflammation and fibrosis in bladder tissues, bladder overactivity, and nociception in a rat model of chemically induced cystitis.

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Abbreviations

HCl:

Hydrochloride

IC:

Interstitial cystitis

MSCs:

Mesenchymal stem cells

PBS:

Phosphate buffered saline

TNF-α:

Tumor necrosis factor-α

TGF-β:

Transforming growth factor-β

VEGF:

Vascular endothelial growth factor

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Acknowledgements

This study was supported by JSPS KAKENHI Grant Number 15K10633.

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Authors and Affiliations

  1. Department of Urology, Jikei University School of Medicine, 3-25-8 Nishishinbashi, Minato-ku, Tokyo, 105-8461, Japan

    Akira Furuta, Taro Igarashi & Shin Egawa

  2. Department of Urology, Nagoya University Graduate School of Medicine, Nagoya, Japan

    Tokunori Yamamoto

  3. Department of Urology, Tokyo Metropolitan Rehabilitation Hospital, Tokyo, Japan

    Yasuyuki Suzuki

  4. Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Naoki Yoshimura

Authors
  1. Akira Furuta
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  2. Tokunori Yamamoto
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  3. Taro Igarashi
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  4. Yasuyuki Suzuki
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  5. Shin Egawa
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Correspondence to Akira Furuta.

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Furuta, A., Yamamoto, T., Igarashi, T. et al. Bladder wall injection of mesenchymal stem cells ameliorates bladder inflammation, overactivity, and nociception in a chemically induced interstitial cystitis-like rat model. Int Urogynecol J 29, 1615–1622 (2018). https://doi.org/10.1007/s00192-018-3592-8

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  • DOI: https://doi.org/10.1007/s00192-018-3592-8

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