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Comparative Analysis of Transcriptional Profile Changes in Larval Zebrafish Exposed to Zinc Oxide Nanoparticles and Zinc Sulfate

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Abstract

Many studies of the toxic effects of zinc oxide nanoparticles (ZnO NPs) in aquatic organisms have been performed because of increasing ZnO NP use. However, the toxicological pathways are not understood. In this study, ZnO NPs were found to be more toxic than ZnSO4 to zebrafish larvae, but ZnO NP toxicity did not involve transcript alterations. Biological processes affected by ZnO NPs and ZnSO4 were investigated by performing ingenuity pathway analysis on differently expressed genes in larvae exposed to sub-lethal ZnO NP and ZnSO4 concentrations. We identified upregulated and downregulated differently expressed genes in fish exposed to ZnO NPs and ZnSO4, and found that ZnO NPs slightly induced cell differentiation and pathways associated with the immune system and activated several key genes involved in cancer cell signaling. The results may be key to predicting and elucidating the mechanisms involved in ZnO NP and ZnSO4 toxicity in zebrafish larvae.

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Acknowledgements

This study was supported through grants KK-1608 and KK-1610-04 provided by the Korea Institute of Toxicology (KIT, Korea).

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Authors and Affiliations

  1. System Toxicology Research Center, Korea Institute of Toxicology, Daejeon, 34111, South Korea

    Ryeo-Ok Kim, Byoung-Chul Kim & Woo-Keun Kim

  2. Future Environmental Research Center, Korea Institute of Toxicology, Jinju, 52834, South Korea

    Jin Soo Choi

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  1. Ryeo-Ok Kim
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  2. Jin Soo Choi
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  3. Byoung-Chul Kim
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  4. Woo-Keun Kim
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Correspondence to Woo-Keun Kim.

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Kim, RO., Choi, J.S., Kim, BC. et al. Comparative Analysis of Transcriptional Profile Changes in Larval Zebrafish Exposed to Zinc Oxide Nanoparticles and Zinc Sulfate. Bull Environ Contam Toxicol 98, 183–189 (2017). https://doi.org/10.1007/s00128-016-1995-0

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  • DOI: https://doi.org/10.1007/s00128-016-1995-0

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