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The Hessian fly resistance gene HvRHF1 is localized in an NBS-LRR gene cluster in barley

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Abstract

Hessian fly (Mayetiola destructor Say) is a significant pest in cereal crops, causing substantial yield losses worldwide. While host resistance is the most efficient method for pest control, research on genetic characterization of Hessian fly resistance in barley (Hordeum vulgare L.) has been limited, and the underlying resistance mechanism remains largely unknown. In this study, we conducted fine mapping of a crucial Hessian fly resistance locus, known as HvRHF1, using a biparental population. Assisted with genetic markers and robust phenotyping assay, we pinpointed the HvRHF1 gene to an ~ 82 kb region on chromosome 4H. Gene prediction and annotation revealed that the HvRHF1 locus comprises three complete NBS-LRR genes, which are characteristic of disease resistance genes. As a result, our study not only provides valuable resources for resistance in barley and genetic tools for breeding, but also identifies candidate genes that lay the foundation for cloning HvRHF1. This endeavor will significantly contribute to our understanding of the molecular mechanisms underlying cereal resistance to Hessian fly.

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Acknowledgements

The authors thank Kirk Anderson for the help with HF infestation and phenotyping; and Megan Overlander-Chen, Xiaohong (Sherry) Jiang, and Abby d'Eustachio for technical assistance.

Funding

This work was supported by the U.S. Department of Agriculture, Agricultural Research Service through project 3060-21000-046-000D. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58102, USA

    Manila Karki, Zhaohui Liu & Shengming Yang

  2. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58102, USA

    Md Golam Robbani, Chenggen Chu & Shengming Yang

  3. Sugarbeet and Potato Research Unit, Edward T. Schafer Agricultural Research Center, USDA-ARS, Fargo, ND, 58102, USA

    Chenggen Chu

  4. Crop Improvement and Genetics Research Unit, Western Regional Research Center, USDA-ARS, Albany, CA, 94710, USA

    Steven Xu

  5. Cereals Crops Research Unit, Edward T. Schafer Agricultural Research Center, USDA-ARS, Fargo, ND, 58102, USA

    Shengming Yang

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  1. Manila Karki
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Contributions

SY conceived and designed the experiments. MK, MGR, and SY performed the experiments. All authors analyzed the data. All authors commented on the first draft wrote by MK and SY.

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Correspondence to Shengming Yang.

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Communicated by Peter Langridge.

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Karki, M., Robbani, M.G., Chu, C. et al. The Hessian fly resistance gene HvRHF1 is localized in an NBS-LRR gene cluster in barley. Theor Appl Genet 137, 71 (2024). https://doi.org/10.1007/s00122-024-04581-5

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