Abstract
Key message
We mapped a new race-specific seedling stripe rust resistance gene on wheat chromosome 5BL and a new APR locus QYr.hazu-2BS from CIMMYT wheat line Kfa/2*Kachu.
Abstract
Breeding resistant wheat (Triticum aestivum) varieties is the most economical and efficient way to manage wheat stripe rust, but requires the prior identification of new resistance genes and development of associated molecular markers for marker-assisted selection. To map stripe rust resistance loci in wheat, we used a recombinant inbred line population generated by crossing the stripe rust-resistant parent ‘Kfa/2*Kachu’ and the susceptible parent ‘Apav#1’. We employed genotyping-by-sequencing and bulked segregant RNA sequencing to map a new race-specific seedling stripe rust resistance gene, which we designated YrK, to wheat chromosome arm 5BL. TraesCS5B02G330700 encodes a receptor-like kinase and is a high-confidence candidate gene for YrK based on virus-induced gene silencing results and the significant induction of its expression 24 h after inoculation with wheat stripe rust. To assist breeding, we developed functional molecular markers based on the polymorphic single nucleotide polymorphisms in the coding sequence region of YrK. We also mapped four adult plant resistance (APR) loci to wheat chromosome arms 1BL, 2AS, 2BS and 4AL. Among these APR loci, we determined that QYr.hazu-1BL and QYr.hazu-2AS are allelic to the known pleiotropic resistance gene Lr46/Yr29/Pm39 and the race-specific gene Yr17, respectively. However, QYr.hazu-2BS is likely a new APR locus, for which we converted closely linked SNP polymorphisms into breeder-friendly Kompetitive allele-specific PCR (KASP) markers. In the present study, we provided new stripe rust resistance locus/gene and molecular markers for wheat breeder to develop rust-resistant wheat variety.
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We thank Planteditors for helping to polishing the manuscript.
Funding
This work was supported by the National Natural Science Foundation of China (Grant No. 31861143010), the Development and application of new wheat varieties with good quality and high yield for the Middle and Lower Reaches of the Yangtze River (NK2022060601), the Key Research and Development Program of Hubei Province (2022BBA0035), Hubei Hongshan Laboratory (2022hspy001, 2021hskf008 and 2022hspy010), a HZAU-AGIS Cooperation Fund (SZYJY2022035), the Australian Grains Research and Development Corporation (GRDC) with funding to the Australian Cereal Rust Control Program (ACRCP) and the CGIAR Research Program WHEAT (CRP-WHEAT).
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YRY performed the experiments, analysed the data and drafted the manuscript. CY and YCZ performed the experiments, analysed the data and revised the manuscript. SDL, BB, LW and YR provided technical assistance. RPS provided germplasm resources. CXL conceived the project, designed the research and revised the manuscript. All of the authors have read and approved the final version of the manuscript.
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122_2023_4432_MOESM1_ESM.jpg
Supplementary Fig. 1 Phenotype of susceptible parent Apav#1 and single gene line for YrK (RIL 977) against Chinese Pst race CYR33 at both seedling (A) and adult plant (B) stages (JPG 199 kb)
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Yin, Y., Yuan, C., Zhang, Y. et al. Genetic analysis of stripe rust resistance in the common wheat line Kfa/2*Kachu under a Chinese rust environment. Theor Appl Genet 136, 185 (2023). https://doi.org/10.1007/s00122-023-04432-9
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DOI: https://doi.org/10.1007/s00122-023-04432-9