Abstract
Key message
We identified and fine-mapped S58, a selfish genetic locus from Asian rice that confers hybrid male sterility in crosses between Asian and African cultivated rice, and found a natural neutral allele in Asian rice lines that will be useful for overcoming S58-mediated hybrid sterility.
Abstract
Hybrids between Asian cultivated rice (Oryza sativa L.) and African cultivated rice (Oryza glaberrima Steud) display severe hybrid sterility (HS), hindering the utilization of strong heterosis in hybrids between these species. Several African rice selfish loci causing HS in Asian–African cultivated rice hybrids have been identified, but few such Asian rice selfish loci have been found. In this study, we identified an Asian rice selfish locus, S58, which causes hybrid male sterility (HMS) in hybrids between the Asian rice variety 02428 and the African rice line CG14. Genetic analysis confirmed that S58 causes a transmission advantage for the Asian rice S58 allele in the hybrid offspring. Genetic mapping with near-isogenic lines and DNA markers delimited S58 to 186 kb and 131 kb regions of chromosome 1 in 02428 and CG14, respectively, and revealed complex genomic structural variation over these mapped regions. Gene annotation analysis and expression profiling analyses identified eight anther-expressed candidate genes potentially responsible for S58-mediated HMS. Comparative genomic analysis determined that some Asian cultivated rice varieties harbor a 140 kb fragment deletion in this region. Hybrid compatibility analysis showed that this large deletion allele in some Asian cultivated rice varieties can serve as a natural neutral allele, S58-n, that can overcome S58-mediated interspecific HMS. Our study demonstrates that this selfish genetic element from Asian rice is important for HMS between Asian and African cultivated rice, broadening our understanding of interspecific HS. This study also provides an effective strategy for overcoming HS in future interspecific rice breeding.
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Data supporting the findings of this work are available within the paper and its Supplementary Information files.
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Acknowledgements
The authors would like to thank our laboratory coworkers for assistance with help growing plant materials and surveys of pollen fertility.
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This research was supported by grants from the National Nature Science Foundation of China (32270671 and 31991222), the Double First-class Discipline Promotion Project (2021B10564001), and the Guangdong Natural Science Funds for Distinguished Young Scholars (2021B1515020089).
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YX and YF designed all experiments and analyzed the data; YF, JT and RL performed most of the experimental work; YX wrote the manuscript. YL and LC provided constructive advice regarding the experiments and manuscript preparation.
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Feng, Y., Tang, J., Liu, R. et al. Characterization and fine-mapping of a new Asian rice selfish genetic locus S58 in Asian–African rice hybrids. Theor Appl Genet 136, 87 (2023). https://doi.org/10.1007/s00122-023-04348-4
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DOI: https://doi.org/10.1007/s00122-023-04348-4