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RIP2 interacts with REL1 to control leaf architecture by modulating brassinosteroid signaling in rice

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Abstract

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RIP2 serves as a negative regulator of leaf inclination through the coordination of BR signaling in rice.

Abstract

Leaf angle is considered as an important morphological trait in rice. Appropriate leaf angle increases the efficiency of sunlight capture and maintains a high level of photosynthesis, ultimately improving crop yield. Our present study demonstrates that RIP2 encodes a RING finger E3 ligase protein that directly binds to ROLLED AND ERECT LEAF 1 (REL1), a key regulator of leaf morphogenesis. Further studies reveal that RIP2 is extensively involved in leaf inclination through the coordination of BR signaling. Repression of RIP2 led to altered phenotypes, including enlarged leaf inclination and fewer tillers. Conversely, rice overexpressing RIP2 exhibited erect leaves. The double mutant rel1 rip2 displayed phenotypes similar to those of rel1, characterized by rolled leaves. Transcriptome profiling of WT, rel1, rip2, and rel1 rip2 mutants revealed that BR and IAA signaling pathways were impaired in rip2. Moreover, rel1, rip2, and rel1 rip2 were insensitive to BR treatment. In summary, these findings demonstrate that RIP2 serves as a negative regulator of leaf inclination, and therefore, provides an approach for the optimization of an ideal plant type.

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Acknowledgements

This study was supported by the Key-Area Research and Development Program of Guangdong Province (2018B020202012), and the Natural Science Foundation of China (31671645).

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  1. Qiuxin Zhang, Guanming Liu, Jing Jin contributed equally to the work.

Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China

    Qiuxin Zhang, Jing Jin, Jiayan Liang, Jingjing Zhang, Haifeng Peng, Wenyi Wang & Zemin Zhang

  2. College of Agriculture & Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510550, China

    Guanming Liu

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  1. Qiuxin Zhang
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Contributions

QZ and ZZ designed the project; QZ, GL, JJ, and JL performed the experiments; QZ, GL, JZ, HP, WW, and ZZ analyzed and interpreted the data; QZ, WW and ZZ wrote the paper.

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Correspondence to Wenyi Wang or Zemin Zhang.

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Communicated by Matthias Wissuwa.

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Zhang, Q., Liu, G., Jin, J. et al. RIP2 interacts with REL1 to control leaf architecture by modulating brassinosteroid signaling in rice. Theor Appl Genet 135, 979–991 (2022). https://doi.org/10.1007/s00122-021-04011-w

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