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Dissection and validation of a QTL cluster linked to Rht-B1 locus controlling grain weight in common wheat (Triticum aestivum L.) using near-isogenic lines

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Abstract

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This study dissected and validated a QTL cluster associated with thousand grain weight on chromosome 4B using multiple near-isogenic lines in common wheat.

Abstract

Grain size and weight are crucial components of wheat yield. Previously, we identified a QTL cluster for thousand grain weight (TGW) on chromosome 4B using the Nongda3338 (ND3338)/Jingdong6 (JD6) doubled haploid population. Here, near-isogenic lines (NILs) in the ND3338 background were developed to dissect and validate the QTL cluster. Based on six independent BC3F3:4 heterogeneous inbred families, the 4B QTL cluster was divided into two linked QTL intervals (designated 4B.1 and 4B.2 QTL). For the 4B.1 QTL, the Rht-B1 gene, of which Rht-B1b allele reduces plant height (PH) by 21.18–29.34 cm (34.34–53.71%), was demonstrated to be the most likely candidate gene with pleiotropic effects on grain size and TGW. For the 4B.2 QTL, the NILJD6 consistently showed an increase in TGW of 3.51–7.68 g (8.84–22.77%) compared with NILND3338 across different field trials, along with a significant increase in PH of 2.26–6.71 cm (3.92–12.01%). Moreover, both QTL intervals had a larger effect on grain width than on grain length. Additionally, the first significant difference in 100-grain fresh weight and 100-grain dry weight between the NIL pairs of the 4B.1 QTL interval (Rht-B1) was observed at 6 days after pollination (DAP), while the differences were first visible at 30 DAP for the 4B.2 QTL interval. Collectively, our work provides a new example of QTL dissection for grain weight in wheat and lays a foundation for further map-based cloning of the major QTL that have potential applications in wheat molecular breeding for high yield.

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Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (Grant No. 2016YFD0100402) and China Postdoctoral Science Foundation (2018M641541).

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Contributions

HP and PG conceived the project, designed the experiments and wrote the manuscript. PG, XS and QM carried out experiments; YZ and YC performed molecular marker development; YW, XW, WM and YG participated in field experiments and phenotypic assessments; XC and AZ provided valuable assistances with field trial preparation; MX, ZH, YY, ZN and QS provided extensive revision and assisted in revising the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Huiru Peng.

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The authors declare that they have no conflict of interest.

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Communicated by Gary Muehlbauer.

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Figure S1

Distribution of polymorphic SNPs per chromosome between the NIL1 pairs based on the wheat 660K SNP array analysis (a), and the distribution of polymorphic SNPs on chromosome 4B according to IWGSC RefSeq v1.0 (b). After filtering the low-confidence heterozygous SNPs, high-quality homozygous SNP variants (y-axis label) between the NIL pairs were used in this study. (TIFF 744 kb)

Figure S2

Distribution of polymorphic SNPs per chromosome between the NIL5 pairs (a) and NIL6 pairs (b) based on the wheat 660K SNP array analyses, and the physical positions of the SNPs on chromosome 4B according to the IWGSC RefSeq v1.0. After filtering the low-confidence heterozygous SNPs, high-quality homozygous SNP variants (y-axis label) between the NIL pairs were used in this study. (TIFF 711 kb)

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Guan, P., Shen, X., Mu, Q. et al. Dissection and validation of a QTL cluster linked to Rht-B1 locus controlling grain weight in common wheat (Triticum aestivum L.) using near-isogenic lines. Theor Appl Genet 133, 2639–2653 (2020). https://doi.org/10.1007/s00122-020-03622-z

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  • DOI: https://doi.org/10.1007/s00122-020-03622-z

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