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Genetic analysis of net form net blotch resistance in barley lines CIho 5791 and Tifang against a global collection of P. teres f. teres isolates

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Abstract

Key message

A CIho 5791 × Tifang recombinant inbred mapping population was developed and used to identify major dominant resistance genes on barley chromosomes 6H and 3H in CI5791 and on 3H in Tifang.

Abstract

The barley line CIho 5791 confers high levels of resistance to Pyrenophora teres f. teres, causal agent of net form net blotch (NFNB), with few documented isolates overcoming this resistance. Tifang barley also harbors resistance to P. teres f. teres which was previously shown to localize to barley chromosome 3H. A CIho 5791 × Tifang F6 recombinant inbred line (RIL) population was developed using single seed descent. The Illumina iSelect SNP platform was used to identify 2562 single nucleotide polymorphism (SNP) markers across the barley genome, resulting in seven linkage maps, one for each barley chromosome. The CIho 5791 × Tifang RIL population was evaluated for NFNB resistance using nine P. teres f. teres isolates collected globally. Tifang was resistant to four of the isolates tested whereas CIho 5791 was highly resistant to all nine isolates. QTL analysis indicated that the CIho 5791 resistance mapped to chromosome 6H whereas the Tifang resistance mapped to chromosome 3H. Additionally, CIho 5791 also harbored resistance to two Japanese isolates that mapped to a 3H region similar to that of Tifang. SNP markers and RILs harboring both 3H and 6H resistance will be useful in resistance breeding against NFNB.

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Acknowledgments

The authors would like to thank Danielle Holmes for technical assistance. This research was supported by funding from USDA-ARS CRIS Project 5442-22000-048-00D, USDA-NIFA-AFRI grant#2011-68002-30029 (T-CAP), the North Dakota Barley Council, and the Montana Wheat and Barley Committee. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Department of Plant Pathology, North Dakota State University, Fargo, ND, USA

    V. M. Koladia, J. K. Richards, R. S. Brueggeman & T. L. Friesen

  2. Cereal Crops Research Unit, Northern Crop Science Laboratory, USDA-ARS, Fargo, ND, USA

    J. D. Faris, S. Chao & T. L. Friesen

Authors
  1. V. M. Koladia
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  2. J. D. Faris
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  3. J. K. Richards
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  4. R. S. Brueggeman
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  5. S. Chao
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  6. T. L. Friesen
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Corresponding author

Correspondence to T. L. Friesen.

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The authors declare that there is no conflicts of interest for this article.

Ethical standards

All experiments performed complied with the ethical standards of the USDA-ARS and North Dakota State University.

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Communicated by F. Ordon.

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Koladia, V.M., Faris, J.D., Richards, J.K. et al. Genetic analysis of net form net blotch resistance in barley lines CIho 5791 and Tifang against a global collection of P. teres f. teres isolates. Theor Appl Genet 130, 163–173 (2017). https://doi.org/10.1007/s00122-016-2801-4

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  • DOI: https://doi.org/10.1007/s00122-016-2801-4

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