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A single base substitution in BADH/AMADH is responsible for fragrance in cucumber (Cucumis sativus L.), and development of SNAP markers for the fragrance

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Sequence analysis revealed that an SNP (A1855G) in CsBADH of cucumber accession PK2011T202 causes amino acid change in a highly conserved motif, Y163C. Gene mapping showed association between the SNP and the fragrance.

Abstract

Pandan-like fragrance is a value-added trait in several food crops such as rice, vegetable soybean and sorghum. The fragrance is caused by the volatile chemical 2-acetyl-1-pyrroline (2AP). Mutation(s) in betaine aldehyde dehydrogenase 2 (BADH2; also known as aminoaldehyde dehydrogenase 2) gene causes defective BADH2 and results in biosynthesis of 2AP. Recently, cucumber cultivars possessing pandan-like fragrance were discovered in Thailand. In this study, we report an association between CsBADH and the fragrance in cucumber accession “PK2011T202”. Gene expression analysis of CsBADH in leaves of PK2011T202 and “301176” (non-fragrant) at various growth stages revealed that CsBADH was expressed in both accessions. Sequence comparison of CsBADH showed that PK2011T202 possesses a single base substitution (A1855G) in exon 5 which causes an amino acid change in a highly conserved motif of BADH, Y163C. Single nucleotide-amplified polymorphism markers were developed to detect the SNP polymorphism between the wild-type and fragrance alleles. Since CsBADH is located on chromosome 1, quantitative trait locus (QTL) mapping was conducted for this chromosome using an F2 and a backcross populations developed from the cross between PK2011T202 and 301176. QTL analysis in both populations showed that the major QTL for fragrance, qFgr, was co-localized with the CsBADH. We concluded that the defect function of CsBADH is responsible for fragrance in cucumber PK2011T202.

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Acknowledgments

This research was supported by the Royal Golden Jubilee Ph.D. Scholarship and the TRF Research Career Development Grant (RSA5580057) both of which co-funded by the Thailand Research Fund (TRF) and Kasetsart University to P. Somta and C. Yundaeng.

Ethical standards and conflict of interest

All the experiments performed in this study comply with the current laws of Thailand. The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Program in Plant Breeding, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand

    Chutintorn Yundaeng & Sompong Chankaew

  2. Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand

    Prakit Somta & Peerasak Srinives

  3. National Center for Genetic Engineering and Biotechnology, 113 Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathumthani, 12120, Thailand

    Sithichoke Tangphatsornruang

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  1. Chutintorn Yundaeng
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  2. Prakit Somta
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  4. Sompong Chankaew
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Correspondence to Prakit Somta.

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Communicated by A. J. Bervillé.

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Yundaeng, C., Somta, P., Tangphatsornruang, S. et al. A single base substitution in BADH/AMADH is responsible for fragrance in cucumber (Cucumis sativus L.), and development of SNAP markers for the fragrance. Theor Appl Genet 128, 1881–1892 (2015). https://doi.org/10.1007/s00122-015-2554-5

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  • DOI: https://doi.org/10.1007/s00122-015-2554-5

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