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Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates

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Abstract

To further our understanding of the genetic control of blast resistance in rice cultivar Gumei 2 and, consequently, to facilitate the utilization of this durably blast-resistant cultivar, we studied 304 recombinant inbred lines of indica rice cross Zhong 156/Gumei 2 and a linkage map comprising 181 markers. An analysis of segregation for resistance against five isolates of rice blast suggested that one gene cluster and three additional major genes that are independently inherited are responsible for the complete resistance of Gumei 2. The gene cluster was located to chromosome 6 and includes two genes mapped previously, Pi25(t), against Chinese rice blast isolate 92-183 (race ZC15) and Pi26(t) against Philippine rice blast isolate Ca89 (lineage 4), and a gene for resistance against Philippine rice blast isolate 92330-5 (lineage 17). Of the two genes conferring resistance against the Philippine isolates V86013 (lineage 15) and C923-39 (lineage 46), we identified one as Pi26(t) and mapped the other onto the distal end of chromosome 2 where Pib is located. We used three components of partial blast resistance, percentage diseased leaf area (DLA), lesion number and lesion size, all measured in the greenhouse, to measure the degree of susceptibility to isolates Ca89 and C923-39 and subsequently identified nine and eight quantitative trait loci (QTLs), respectively. Epistasis was determined to play an important role in partial resistance against Ca89. Using DLA measured on lines susceptible in a blast nursery, we detected six QTLs. While different QTLs were detected for partial resistance to Ca89 and C923-39, respectively, most were involved in the partial resistance in the field. Our results suggest that the blast resistance in Gumei 2 is controlled by multiple major genes and minor genes with epistatic effects.

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Acknowledgements

The authors would like to thank Dr. Vera Cruz for comprehensive discussion, Prof. Jan Leach for providing the candidate gene probes, Mr. Rong-Yao Chai for testing blast resistance in the Zhejiang Academy of Agricultural Sciences, and the Cornell University (Ithaca, New York State, USA) and Japanese Genome Research Program for providing the DNA probes. We would like to acknowledge the help from colleagues in the Asian Rice Biotechnology Network (ARBN) laboratory and Genetics laboratory, Entomology and Plant Pathology Division (EPPD), IRRI. This work was supported by ARBN, Chinese 863 program (2003AA207030), and Rockefeller Foundation International Rice Biotechnology Program.

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Authors and Affiliations

  1. Chinese National Centre for Rice Improvement and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China

    J.-L. Wu, Y.-Y. Fan, K.-L. Zheng & J.-Y. Zhuang

  2. Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, China

    D.-B. Li

  3. Entomology and Plant Pathology Division, International Rice Research Institute, DAPO P.O. Box 7777, Metro Manila, Philippines

    J.-L. Wu & H. Leung

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  1. J.-L. Wu
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  2. Y.-Y. Fan
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  3. D.-B. Li
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  4. K.-L. Zheng
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  5. H. Leung
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  6. J.-Y. Zhuang
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Correspondence to J.-Y. Zhuang.

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Communicated by D.J. Mackill

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Wu, JL., Fan, YY., Li, DB. et al. Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates. Theor Appl Genet 111, 50–56 (2005). https://doi.org/10.1007/s00122-005-1971-2

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  • DOI: https://doi.org/10.1007/s00122-005-1971-2

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