Abstract
Interleukin (IL)-6 plays important roles in autoimmunity and inflammation and is essential for T helper (Th) 2 and Th17 differentiation. However, whether it is involved in the development and function of dendritic cells (DCs) during allergen-induced airway inflammation and airway hyper-reactivity (AHR) remains undefined. In this study, Dermatophagoides pteronyssinus (Der p)-induced airway inflammation and AHR were studied in IL-6 knockout (KO) mice. Der p-loaded bone marrow-derived DCs (BMDCs) from IL-6 KO mice were used to assaying their ability to induce airway inflammation in naïve wild-type mice. Our results showed that IL-6 KO mice showed reduced AHR, significant decreases in inflammatory cell recruitment and Th2 and Th17 cytokine production in the airways, and lowered Der p-specific immunoglobulin G1 after Der p exposure. Further exploration of BMDCs from IL-6 KO mice revealed decreased activity of phagocytosis and reduced expression of MHC class II and CD86 after Der p stimulation. Adoptive transfer of Der p-loaded BMDCs from IL-6 KO mice also showed a functional defect in their inability to induce Th2 and Th17 immune responses and trigger airway inflammation and AHR in recipient mice. Finally, in allergic asthmatics, DCs that differentiated from monocytes treated with anti-IL-6 receptor antibody (tocilizumab) had poor capacity for eliciting Th2 polarization as compared to DCs generated from monocytes without antibody treatment. In conclusion, IL-6 signaling in DCs is essential for their uptake of allergens, maturation, and initiation of Th2/Th17-mediated airway inflammation and AHR in asthma, thus providing a new potential target for treating allergic asthma.
Key messages
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IL-6 signaling is important for DCs to take up allergens and to initiate Th2/Th17-mediated airway inflammation.
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DCs from allergic asthmatics treated with anti-IL-6 receptor antibody had poor capacity for eliciting Th2 polarization.
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Anti-IL-6 treatment may provide a new potential target for treating allergic asthma.
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References
Gould HJ, Sutton BJ (2008) IgE in allergy and asthma today. Nat Rev Immunol 8:205–217
Kay AB (2001) Allergy and allergic diseases. First of two parts. N Engl J Med 344:30–37
Wills-Karp M (2004) Interleukin-13 in asthma pathogenesis. Immunol Rev 202:175–190
McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A et al (2008) TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice. J Immunol 181:4089–4097
Wilson RH, Whitehead GS, Nakano H, Free ME, Kolls JK, Cook DN (2009) Allergic sensitization through the airway primes Th17-dependent neutrophilia and airway hyperresponsiveness. Am J Respir Crit Care Med 180:720–730
Rahman MS, Yamasaki A, Yang J, Shan L, Halayko AJ, Gounni AS (2006) IL-17A induces eotaxin-1/CC chemokine ligand 11 expression in human airway smooth muscle cells: role of MAPK (Erk1/2, JNK, and p38) pathways. J Immunol 177:4064–4071
Wakashin H, Hirose K, Maezawa Y, Kagami S, Suto A, Watanabe N, Saito Y, Hatano M, Tokuhisa T, Iwakura Y et al (2008) IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice. Am J Respir Crit Care Med 178:1023–1032
Bettelli E, Carrier Y, Gao W, Korn T, Strom TB, Oukka M, Weiner HL, Kuchroo VK (2006) Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells. Nature 441:235–238
Veldhoen M, Hocking RJ, Atkins CJ, Locksley RM, Stockinger B (2006) TGFbeta in the context of an inflammatory cytokine milieu supports de novo differentiation of IL-17-producing T cells. Immunity 24:179–189
Diehl S, Anguita J, Hoffmeyer A, Zapton T, Ihle JN, Fikrig E, Rincón M (2000) Inhibition of Th1 differentiation by IL-6 is mediated by SOCS1. Immunity 13:805–815
Diehl S, Chow CW, Weiss L, Palmetshofer A, Twardzik T, Rounds L, Serfling E, Davis RJ, Anguita J, Rincón M (2002) Induction of NFATc2 expression by interleukin 6 promotes T helper type 2 differentiation. J Exp Med 196:39–49
Broide DH, Lotz M, Cuomo AJ, Coburn DA, Federman EC, Wasserman SI (1992) Cytokines in symptomatic asthma airways. J Allergy Clin Immunol 89:958–967
Konno S, Gonokami Y, Kurokawa M, Kawazu K, Asano K, Okamoto K, Adachi M (1996) Cytokine concentrations in sputum of asthmatic patients. Int Arch Allergy Immunol 109:73–78
Marini M, Vittori E, Hollemborg J, Mattoli S (1992) Expression of the potent inflammatory cytokines, granulocyte-macrophage-colony-stimulating factor and interleukin-6 and interleukin-8, in bronchial epithelial cells of patients with asthma. J Allergy Clin Immunol 89:1001–1009
Yokoyama A, Kohno N, Fujino S, Hamada H, Inoue Y, Fujioka S (1995) Circulating interleukin-6 levels in patients with bronchial asthma. Am J Respir Crit Care Med 151:1354–1358
Chen CL, Wang SD, Zeng ZY, Lin KJ, Kao ST, Tani T, Yu CK, Wang JY (2006) Serine protease inhibitors nafamostat mesilate and gabexate mesilate attenuate allergen-induced airway inflammation and eosinophilia in a murine model of asthma. J Allergy Clin Immunol 118:105–112
Kushwah R, Oliver JR, Wu J, Chang Z, Hu J (2011) Elf3 regulates allergic airway inflammation by controlling dendritic cell-driven T cell differentiation. J Immunol 187:4639–4653
DiCosmo BF, Geba GP, Picarella D, Elias JA, Rankin JA, Stripp BR, Whitsett JA, Flavell RA (1994) Airway epithelial cell expression of interleukin-6 in transgenic mice. Uncoupling of airway inflammation and bronchial hyperreactivity. J Clin Invest 94:2028–2035
Kuhn C 3rd, Homer RJ, Zhu Z, Ward N, Flavell RA, Geba GP, Elias JA (2000) Airway hyperresponsiveness and airway obstruction in transgenic mice. Morphologic correlates in mice overexpressing interleukin (IL)-11 and IL-6 in the lung. Am J Respir Cell Mol Biol 22:289–295
Cates EC, Fattouh R, Wattie J, Inman MD, Goncharova S, Coyle AJ, Gutierrez-Ramos JC, Jordana M (2004) Intranasal exposure of mice to house dust mite elicits allergic airway inflammation via a GM-CSF-mediated mechanism. J Immunol 173:6384–6392
Krishnamoorthy N, Oriss TB, Paglia M, Fei M, Yarlagadda M, Vanhaesebroeck B (2008) Activation of c-Kit in dendritic cells regulates T helper cell differentiation and allergic asthma. Nat Med 14:565–573
Sporik R, Chapman MD, Platts-Mills TA (1992) House dust mite exposure as a cause of asthma. Clin Exp Allergy 22:897–906
Thomas WR, Hales BJ, Smith WA (2010) House dust mite allergens in asthma and allergy. Trends Mol Med 16:321–328
Hammad H, Plantinga M, Deswarte K, Pouliot P, Willart MA, Kool M, Muskens F, Lambrecht BN (2010) Inflammatory dendritic cells—not basophils—are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen. J Exp Med 207:2097–2111
Genovese MC, McKay JD, Nasonov EL, Mysler EF, da Silva NA, Alecock E, Woodworth T, Gomez-Reino JJ (2008) Interleukin-6 receptor inhibition with tocilizumab reduces disease activity in rheumatoid arthritis with inadequate response to disease-modifying antirheumatic drugs: the tocilizumab in combination with traditional disease-modifying antirheumatic drug therapy study. Arthritis Rheum 58:2968–2980
Kurashima K, Tamura J, Fujimura M, Qiu Z, Nakao S, Mukaida N (2005) Reduced serum antibody production and acute airway inflammation in interleukin 6-deficient mice challenged with ovalbumin. Allergol Int 54:331–338
Kuperman DA, Huang X, Koth LL, Chang GH, Dolganov GM, Zhu Z, Elias JA, Sheppard D, Erle DJ (2002) Direct effects of interleukin-13 on epithelial cells cause airway hyperreactivity and mucus overproduction in asthma. Nat Med 8:885–889
Neveu WA, Allard JB, Dienz O, Wargo MJ, Ciliberto G, Whittaker LA, Rincon M (2009) IL-6 is required for airway mucus production induced by inhaled fungal allergens. J Immunol 183:1732–1738
Langdon C, Kerr C, Tong L, Richards CD (2003) Oncostatin M regulates eotaxin expression in fibroblasts and eosinophilic inflammation in C57BL/6 mice. J Immunol 170:548–555
Qiu Z, Fujimura M, Kurashima K, Nakao S, Mukaida N (2004) Enhanced airway inflammation and decreased subepithelial fibrosis in interleukin 6-deficient mice following chronic exposure to aerosolized antigen. Clin Exp Allergy 34:1321–1328
Santiago-Schwarz F, Tucci J, Carsons SE (1996) Endogenously produced interleukin 6 is an accessory cytokine for dendritic cell hematopoiesis. Stem Cells 14:225–231
Bleier JI, Pillarisetty VG, Shah AB, DeMatteo RP (2004) Increased and long-term generation of dendritic cells with reduced function from IL-6-deficient bone marrow. J Immunol 172:7408–7416
Lewkowich IP, Lajoie S, Clark JR, Herman NS, Sproles AA, Wills-Karp M (2008) Allergen uptake, activation, and IL-23 production by pulmonary myeloid DCs drives airway hyperresponsiveness in asthma-susceptible mice. PLoS One 3, e3879
Park SJ, Nakagawa T, Kitamura H, Atsumi T, Kamon H, Sawa S, Kamimura D, Ueda N, Iwakura Y, Ishihara K et al (2004) IL-6 regulates in vivo dendritic cell differentiation through STAT3 activation. J Immunol 173:3844–3854
Huang HJ, Lin YL, Liu CF, Kao HF, Wang JY (2011) Mite allergen decreases DC-SIGN expression and modulates human dendritic cell differentiation and function in allergic asthma. Mucosal Immunol 4:519–527
Acknowledgments
This work was supported by the Ministry of Science and Technology (MOST), Taiwan, under grant number NSC 96-2314-B-006-027-MY3.
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YLL designed the research, performed the studies, analyzed the data, interpreted the results, and wrote the manuscript. SHC designed the research and revised the manuscript. JYW designed the research, interpreted the results, and revised the manuscript.
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Lin, YL., Chen, SH. & Wang, JY. Critical role of IL-6 in dendritic cell-induced allergic inflammation of asthma. J Mol Med 94, 51–59 (2016). https://doi.org/10.1007/s00109-015-1325-8
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DOI: https://doi.org/10.1007/s00109-015-1325-8