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Efficacy of glutathione mesotherapy in burns: an experimental study

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European Journal of Trauma and Emergency Surgery Aims and scope Submit manuscript

Abstract

Background

Thermal burns are the leading cause of trauma worldwide. Currently, no consensus on optimal treatment of deep partial-thickness (second-degree) burns has emerged, as reflected by the wide variability in available wound-care materials. The relative efficacies of products used for treatment of partial-thickness thermal burns remain unclear. Mesotherapy features intradermal administration of various agents, depending on burn location. In the present experimental study, we explored the efficacy of mesotherapy used to treat partial-thickness thermal burns in 50 male Wistar rats divided into five groups of equal number. No procedure was performed after infliction of thermal burns in control group (Group 1). Mesotherapy was applied with physiological saline in sham group (Group 2), glutathione, taurine, and l-carnitine were separately applied in Group 3, Group 4, and Group 5, respectively.

Materials and methods

Mesotherapeutic agents were injected intradermally into the reticular layer of the dermis using the point technique. The first course of mesotherapy was given within the first 2 h after infliction of thermal burns, and therapy was continued to day 10. On day 22, unhealed thermal burn areas were measured prior to sacrifice, and biopsies covering the total areas of burns were performed to allow of pathological evaluation.

Results

Group 3 (the glutathione group) showed the best extent of healing, followed by Group 4 (the taurine group) and Group 5 (the l-carnitine group). The healed thermal burn areas in these groups were significantly greater than those in the control and sham groups (P = 0.001). All of healing, acute and chronic inflammation, the amount of granulation tissue, the level of fibroblast maturation, the amount of collagen, the extent of re-epithelization and neovascularization, and ulcer depth were scored upon pathological examination of tissue cross-sections. The best outcomes were evident in the glutathione group, with statistical significance. Although wound healing in the l-carnitine and taurine groups was better than in the control and sham groups, the differences were not statistically significant.

Conclusion

Thus, glutathione mesotherapy was effective when used to treat partial-thickness thermal burns and may be a useful treatment option for various human burns.

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Authors and Affiliations

  1. Department of Plastic, Reconstructive and Aesthetic Surgery, Bulent Ecevit University Medical Faculty, A blok Kat:3, Kozlu, Zonguldak, Turkey

    A. Buz, T. Görgülü, A. Olgun & E. Kargi

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  1. A. Buz
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Correspondence to T. Görgülü.

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Conflict of interest

The authors Ayhan Buz, Tahsin Görgülü, Abdulkerim Olgun, and Eksal Kargi declare that they have no conflict of interest.

Compliance with ethical requirements

The study was approved by the Animal Experiments Local Ethical Committee of the Bulent Ecevit University prior to initiation, and appropriate protocols were followed. The research was conducted in the experimental laboratory of the Medical Faculty of Bulent Ecevit University. All experimental protocols conformed to international regulations and declarations relevant to animal experimentation. Ethical Committee Approval Number: 2010-15-27/05.

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Buz, A., Görgülü, T., Olgun, A. et al. Efficacy of glutathione mesotherapy in burns: an experimental study. Eur J Trauma Emerg Surg 42, 775–783 (2016). https://doi.org/10.1007/s00068-015-0607-8

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  • DOI: https://doi.org/10.1007/s00068-015-0607-8

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