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Leaf waxes from Lathyrus sativus: short-range attractant and stimulant for nymph laying in a viviparous insect

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Abstract

Lathyrus sativus L. (Fabaceae) is an important pulse crop of Asia, Europe, and Africa. Infestation by the aphid, Aphis craccivora Koch (Hemiptera: Aphididae) causes stunted growth of plants and reduces seed production. Females lay nymphs on the leaves and flowers of L. sativus. Hence, it is relevant to study the importance of leaf wax compounds (long-chain alkanes and free fatty acids) from two cultivars [BIO L 212 Ratan (BIO) and Nirmal B-1 (NIR)] of L. sativus as short-range attractant and stimulant for nymph laying in the aphid. The TLC, GC-MS and GC-FID analyses of n-hexane extracts from leaves of two cultivars revealed 18 n-alkanes from n-C15 to n-C36 and 14 free fatty acids from C12:0 to C22:0. Pentadecane was predominant among n-alkanes in both cultivars. Palmitoleic acid and pentadecanoic acid were predominant free fatty acids in leaf waxes of BIO and NIR, respectively. Females were attracted towards leaf waxes of both cultivars compared to the control solvent (n-hexane) in Y-tube olfactometer bioassays. A synthetic blend of either pentadecane, tridecanoic acid, and linoleic acid at similar amounts present in one leaf equivalent wax of BIO, or pentadecane, docosane, pentacosane, heptacosane, tritriacontane, and linoleic acid at similar amounts present in one leaf equivalent wax of NIR acted as short-range attractant and stimulated females to lay nymphs. But, the latter blend was more attractive and stimulated females to lay more nymphs than the former blend, and hence, this latter blend could be employed in the development of baited traps in pest management strategies.

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Acknowledgements

We thank anonymous reviewers and Prof. Dr. Marko Rohlfs, Editor-in-Chief, Chemoecology for many helpful suggestions of earlier versions of the manuscript. We also thank Prof. Samiran Chakrabarti, ex-Professor, Department of Zoology, University of Kalyani, West Bengal for authenticating the insect. The financial assistance from the DST-SERB, New Delhi, Govt. of India to Anandamay Barik [EEQ/2017/000018] is gratefully acknowledged. We are thankful to DST PURSE Phase-II for providing necessary instrumental facilities.

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  1. Ecology Research Laboratory, Department of Zoology, The University of Burdwan, Burdwan, West Bengal, 713 104, India

    Paroma Mitra, Swati Das & Anandamay Barik

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  1. Paroma Mitra
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  2. Swati Das
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  3. Anandamay Barik
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by PM and SD. The first draft of the manuscript was written by AB and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Anandamay Barik.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Marko Rohlfs.

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Mitra, P., Das, S. & Barik, A. Leaf waxes from Lathyrus sativus: short-range attractant and stimulant for nymph laying in a viviparous insect. Chemoecology 30, 117–129 (2020). https://doi.org/10.1007/s00049-020-00303-7

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