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Semiochemistry of Dendroctonus armandi Tsai and Li (Coleoptera: Curculionidae: Scolytinae): both female-produced aggregation pheromone and host tree kairomone are critically important

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Abstract

The Chinese white pine beetle, Dendroctonus armandi Tsai and Li, is one of the most destructive insects in natural and managed forests of Chinese white pine (Pinus armandii Franch.) in China. Female-produced volatiles, as aggregation pheromone candidates, and volatiles released from host pine trees, as possible kairomones, were collected via aeration and hindgut solvent extraction techniques, analyzed by gas chromatography–mass spectrometry (GC–MS), and assayed by field-trapping experiments in Shaanxi and Hubei provinces, China. GC–MS analyses showed that monoterpenes are the major volatile compounds from all aeration samples of P. armandii logs, with or without D. armandi attacks, accounting for >95 % of total volatiles. α-Pinene (63.18 %), Δ3-carene (14.4 %), β-pinene (7.9 %), limonene (6.2 %) and β-myrcene (3.85 %) were the major monoterpenes, while (+)-longifolene (1.1 %) was the most dominant sesquiterpene. Four bark beetle-related volatile compounds, trans-verbenol (tV; major component), exo-brevicomin (EBV), seudenol (SD), and 1-methyl-2-cyclohexen-1-ol (MCOL) were identified from both the aeration extracts of P. armandii logs with D. armandi females and their hindgut extracts. Our field bioassays showed that all four female-produced individual pheromone candidates and their possible combinations (binary, ternary and quaternary) were not significantly attractive to D. armandi. Two pheromone candidate blends [one ternary (tV/SD/EBV) and one quaternary (tV/SD/EBV/MCOL)], and a mixture of host terpene kairomone candidates were also inactive when tested alone. However, when the five major host monoterpenes and one sesquiterpene (mimicking the natural ratio) were combined with the pheromone ternary or quaternary blend, D. armandi trap catches were significantly (4–10 times) higher than captures in the blank control traps or traps baited with the individual blends, indicating a strong synergistic effect. These synergistically attractive semiochemicals are potentially of great utility for monitoring and mass-trapping this serious forest pest of Chinese white pine in western China.

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References

  • Borden JH, Ryker LC, Chong LJ, Pierce HD Jr, Johnston BD, Oehlschlager C (1987) Response of the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Scolytidae), to five semiochemicals in British Columbia lodgepole pine forests. Can J For Res 17:118–128

    Article  CAS  Google Scholar 

  • Borden JH, Pureswaran DS, Lafontaine JP (2008) Synergistic blends of monoterpenes for aggregation pheromones of the mountain pine beetle (Coleoptera: Curculionidae). J Econ Entomol 101:1266–1275

    Article  CAS  PubMed  Google Scholar 

  • Byers JA (1989) Behavioral mechanisms involved in reducing competition in bark beetles. Holarctic Ecol 12:466–476

    Google Scholar 

  • Byers JA (1993) Randomization algorithms in BASIC for experimental design. Comput Biol Med 23:167–176

    Article  CAS  PubMed  Google Scholar 

  • Byers JA, Zhang Q-H (2012) Chemical ecology of bark beetles in regard to search and selection of host trees. In: Liu T-X, Le K (eds) Recent advances in entomological research. Springer, New York, pp 150–190

    Google Scholar 

  • Byers JA, Wood DL, Craig J, Hendry LB (1984) Attractive and inhibitory pheromones produced in the bark beetle, Dendroctonus brevicomis, during host colonization: regulation of inter-and intraspecific competition. J Chem Ecol 10:861–877

    Article  CAS  PubMed  Google Scholar 

  • Chen H, Tang M (2007) Spatial and temporal dynamics of bark beetles in Chinese white pine in Qinling Mountains of Shaanxi Province, China. Environ Entomol 36:1124–1130

    Article  CAS  PubMed  Google Scholar 

  • Chen H, Tang M, Gao J, Chen X, Li Z (2006) Changes in the composition of volatile monoterpenes and sesquiterpenes of Pinus armandii, P. tabulaeformis, and P. bungeana in Northwest China. Chem Nat Comp 42:534–538

    Article  CAS  Google Scholar 

  • Chen M, Li Y-H, Wang X-J (2007) Analysis of volatile constituents in Pinus armandii bark with GC/MS. For Res 20:428–432

    Google Scholar 

  • Fettig CJ, Dabney CP, McKelvey SR, Borys RR (2006) An assessment of re-randomization methods in bark beetle (Scolytidae) trapping bioassays. Agri For Entomol 8:267–271

    Article  Google Scholar 

  • He M-J, Liao C-L, Guo H-J, He Y-S, Liao L-J, Wang H, Zhang Y, Zhang G-H (2009) Analysis of constituents of the volatile materials in the bark of Pinus armandii from Enshi. J Hubei Univ Natl 27:254–257

    CAS  Google Scholar 

  • Hofstetter R, Chen Z, Gaylord M, McMillin J, Wagner M (2008) Synergistic effects of α-pinene and exo-brevicomin on pine bark beetles and associated insects in Arizona. J Appl Entomol 132:387–397

    Article  Google Scholar 

  • Hofstetter RW, Gaylord ML, Martinson S, Wagner MR (2012) Attraction to monoterpenes and beetle-produced compounds by syntopic Ips and Dendroctonus bark beetles and their predators. Agri For Entomol 14:207–215

    Article  Google Scholar 

  • Lanne BS, Schlyter F, Byers JA, Löfqvist J, Leufvén A, Bergström G, Van Der Pers JNC (1987) Differences in attraction to semiochemicals present in sympatric pine shoot beetles, Tomicus minor and T. piniperda. J Chem Ecol 13:1045–1067

    Article  CAS  PubMed  Google Scholar 

  • Li Z-B, Chen H, Chen X (2006) Analysis of chemical constituents of volatile oil from resin of Pinus armandii. J Northwest For Univ 21:138–141

    Google Scholar 

  • Li Y, Chen M, Ye H (2007) Relationship between volatile matters from Armand pine and endangerments of Armand pine bark-weevil. J Northeast For Univ 35:7–10

    Google Scholar 

  • Lindgren B, Gries G, Pierce H Jr, Mori K (1992) Dendroctonus pseudotsugae Hopkins (Coleoptera: Scolytidae): production of and response to enantiomers of 1-methylcyclohex-2-en-1-ol. J Chem Ecol 18:1201–1208

    Article  CAS  PubMed  Google Scholar 

  • Lindgren BS, Miller DR, Lafontaine J-P (2012) MCOL, frontalin and ethanol: a potential operational trap lure for Douglas-fir beetle in British Columbia. J Entomol Soc Brit Col 109:72–74

    Google Scholar 

  • Liu Z, Xu B, Miao Z, Sun J (2013) The pheromone frontalin and its dual function in the invasive bark beetle Dendroctonus valens. Chem Senses 38:485–495

    Article  CAS  PubMed  Google Scholar 

  • Miller DR, Borden JH (2000) Dose-dependent and species-specific responses of pine bark beetles (Coleoptera: Scolytidae) to monoterpenes in association with pheromones. Can Entomol 132:183–195

    Article  Google Scholar 

  • Payne T, Coster J, Richerson J, Edson L, Hart E (1978) Field response of the southern pine beetle to behavioral chemicals. Environ Entomol 7:578–582

    Article  CAS  Google Scholar 

  • Pu X-J, Chen H (2007) Relations between attacking of Dendroctonus armandi and nutrition and resistance material of host trees (Pinus armandii). J Northwest A F Univ 35:106–110

    Google Scholar 

  • Pureswaran DS, Borden JH (2005) Primary attraction and kairomonal host discrimination in three species of Dendroctonus (Coleoptera: Scolytidae). Agric For Entomol 7:219–230

    Article  Google Scholar 

  • Pureswaran DS, Hofstetter RW, Sullivan BT (2008a) Attraction of the southern pine beetle, Dendroctonus frontalis, to pheromone components of the western pine beetle, Dendroctonus brevicomis (Coleoptera: Curculionidae: Scolytinae), in an allopatric zone. Environ Entomol 37:70–78

    Article  CAS  PubMed  Google Scholar 

  • Pureswaran DS, Sullivan BT, Ayres MP (2008b) High individual variation in pheromone production by tree-killing bark beetles (Coleoptera: Curculionidae: Scolytinae). Naturwissenschaften 95:33–44

    Article  CAS  PubMed  Google Scholar 

  • Rudinsky J, Kinzer G, Fentiman A, Foltz R (1972) trans-Verbenol isolated from Douglas-fir beetle: laboratory and field bioassays in Oregon. Environ Entomol 1:485–488

    Article  CAS  Google Scholar 

  • Ryall K, Silk P, Thurston G, Scarr T, de Groot P (2013) Elucidating pheromone and host volatile components attractive to the spruce beetle, Dendroctonus rufipennis (Coleoptera: Curculionidae), in eastern Canada. Can Entomol 145:406–415

    Article  Google Scholar 

  • Schlyter F, Birgersson G (1999) Forest beetles. In: Hardie J, Minks AK (eds) Pheromones in non-lepidopteran insects associated with agricultural plants. CAB International, Oxford, pp 113–148

    Google Scholar 

  • Schlyter F, Zhang Q-H, Liu G-T, Ji L-Z (2001) A successful case of pheromone mass trapping of the bark beetle Ips duplicatus in a forest island, analysed by 20-year time-series data. Integr Pest Manag Rev 6:185–196

    Article  Google Scholar 

  • Seybold SJ, Huber DP, Lee JC, Graves AD, Bohlmann J (2006) Pine monoterpenes and pine bark beetles: a marriage of convenience for defense and chemical communication. Phytochem Rev 5:143–178

    Article  CAS  Google Scholar 

  • Sullivan BT, Dalusky MJ, Mori K, Brownie C (2011) Variable responses by southern pine beetle, Dendroctonus frontalis Zimmermann, to the pheromone component endo-Brevicomin: influence of enantiomeric composition, release rate, and proximity to infestations. J Chem Ecol 37:403–411

    Article  CAS  PubMed  Google Scholar 

  • Wang K-Q, Wang X-B (2010) Dendroctonus armandi Control in Taibai Mountains. Shaanxi For Sci Tech 6:58–60

    Google Scholar 

  • Wang X, Chen H, Ma C, Li Z (2010) Chinese white pine beetle, Dendroctonus armandi (Coleoptera: Scolytinae), population density and dispersal estimated by mark-release-recapture in Qinling Mountains, Shaanxi, China. Appl Entomol Zool 45:557–567

    Article  Google Scholar 

  • Wang R-L, Yang W, Yang Z-Z, Chen X-P, Yang C-P, Li Q, Li F, Chen C-M (2011a) Electroantennographic and behavioral responses of Dendroctonus armandi (Coleoptera: Ipidae) to host plant volatiles. Chin J Ecol 30:724–729

    CAS  Google Scholar 

  • Wang R-L, Yang W, Yang Z-Z, Chen X, Wang XW, Yang C-P (2011b) Electroantennographic and behavioral responses of Dendroctonus armandi (Tsai et Li) to 9 plant volatiles. For Pest Dis 30:23–26

    Google Scholar 

  • Wang X-W, Zhang Z-J, Yang W, Yang C-P, Li F (2014) A preliminary study of the biological character of Dendroctonus armandi. J Sichuan For Sci Tech 35:56–58

    Google Scholar 

  • Wood DL (1982) The role of pheromones, kairomones, and allomones in the host selection and colonization behavior of bark beetles. Ann Rev Entomol 27:411–446

    Article  CAS  Google Scholar 

  • Wu SP, Chen H, Wu Q (2012) Volatile compounds in the frass of adult Chinese white pine beetle. J Northwest A F Univ 27:111–116

    Google Scholar 

  • Xie S-A, Ding Y (2010) Semiochemicals of Dendroctonus armandi. J Northeast For Univ 38:91–94

    CAS  Google Scholar 

  • Xie S-A, Lv S-J (2012) An improved lure for trapping the bark beetle Dendroctonus armandi (Coleoptera: Scolytinae). Euro J Entomol 109:569–577

    Article  CAS  Google Scholar 

  • Xie S-A, Lv S-J (2013) Effect of different semiochemicals blends on spruce bark beetle, Ips typographus (Coleoptera: Scolytidae). Entomol Sci 16:179–190

    Article  Google Scholar 

  • Yang X, Zhao H, Wang J, Meng Q, Zhang H, Yao L, Zhang Y, Dong A, Ma Y, Wang Z (2010) Chemical composition and antioxidant activity of essential oil of pine cones of Pinus armandii from the Southwest region of China. J Med Plant Res 4:1668–1672

    CAS  Google Scholar 

  • Yin H-F, Huang F-S, Li Z-L (1984) Dendroctonus armandi Tsai & Li. Economic insect fauna of China (Coleoptera: Scolytidae), vol 29. Science Press, Beijing, pp 26–35

  • Zhang L, Chen H, Ma C, Tian Z (2010) Electrophysiological responses of Dendroctonus armandi (Coleoptera: Curculionidae: Scolytinae) to volatiles of Chinese white pine as well as to pure enantiomers and racemates of some monoterpenes. Chemoecol 20:265–275

    Article  CAS  Google Scholar 

  • Zhang L, Chen H, Chen X (2011) Effect of Dendroctonus armandi infection on the volatile constituents of Pinus armandii in Qinling Mountains. J Northwest For Univ 26:114–118

    Google Scholar 

Download references

Acknowledgments

We thank Dr. Jeff Aldrich (Jeffrey R. Aldrich consulting, LLC, USA), Dr. John A. Byers (USDA-ARS) and Prof. Fredrik Schlyter (SLU, Sweden) for valuable comments and suggestions on an earlier version of this manuscript.

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Authors and Affiliations

  1. General Station of Forest Pest Management, State Forestry Administration, No. 58, North Huanghe Street, Shenyang, 110034, People’s Republic of China

    Guofa Chen & Yushuang Song

  2. Shaanxi Provincial General Station of Forest Pest Control and Quarantine, No. 233, Xiguanzheng Street, Xi’an, 710082, People’s Republic of China

    Peixin Wang

  3. Hubei Provincial Academy of Forestry Sciences, No. 39, Fenglin Road, Senlin Avenue, Wuhan, 430075, People’s Republic of China

    Jingyuan Chen

  4. Key Laboratory of Forest Protection of State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, 100091, People’s Republic of China

    Zhen Zhang

  5. Hubei Provincial General Station of Forest Pest Control and Quarantine, No. 1 Fangjiazui, Xiongchu Street, Wuhan, 430079, People’s Republic of China

    Shaoming Wang & Xianbin Huang

  6. Sterling International, Inc., 3808 N. Sullivan Rd, Bldg 16, Spokane, WA, 99216-1630, USA

    Qing-He Zhang

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  1. Guofa Chen
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Correspondence to Qing-He Zhang.

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Chen, G., Song, Y., Wang, P. et al. Semiochemistry of Dendroctonus armandi Tsai and Li (Coleoptera: Curculionidae: Scolytinae): both female-produced aggregation pheromone and host tree kairomone are critically important. Chemoecology 25, 135–145 (2015). https://doi.org/10.1007/s00049-014-0182-1

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  • DOI: https://doi.org/10.1007/s00049-014-0182-1

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