Skip to main content
SpringerLink
Log in

Rapid determination of lenalidomide in rat plasma by an ultra performance liquid chromatography/tandem mass spectrometric method

  • Original Research
  • Published:
Medicinal Chemistry Research Aims and scope Submit manuscript

Abstract

A new, rapid, and specific ultra performance liquid chromatographic assay with mass spectrometric detection has been developed and validated for the quantitative determination of the antiangiogenic agent lenalidomide in rat plasma using thalidomide as an internal standard with electro spray ionization in multiple-reaction monitoring mode. The analyte and internal standard were extracted by liquid–liquid extraction using methyl-tert-butyl ether solvent. Chromatography was performed isocratically on Acquity BEH C18 column using a mobile phase consisting of a mixture of buffer, acetonitrile, and formic acid in ratios of 30:70:0.1 (v/v/v). The method had a chromatographic run time of 1.2 min. The calibration curve was fit to a linear response concentration data over a range of 0.06–60.00 μM using a weighting factor of 1/×2. The intra- and inter-day precision and accuracy results were well within the acceptable limits. The validated method was successfully applied for routine analysis of the pharmacokinetics of lenalidomide.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Ayalew T, Jorge C, Srdan V, Ruben AM, Deborah T, Terra LL, William JH, Mark RL, Jacob BA, Dan J, Catriona B, Jerome BZ, Rhett PK, Rebecca FM, Francis G, Hagop MK (2006) Lenalidomide therapy in myelofibrosis with myeloid metaplasia. Blood 108:1158–1164

    Article  Google Scholar 

  • Barlogie B, Shaughnessy J, Tricot G, Jacobson J, Zangari M, Anaissie E, Walker R, Crowley J (2004) Treatment of multiple myeloma. Blood 103:20–32

    Article  CAS  PubMed  Google Scholar 

  • Chanan-Khan A, Miller KC, Musial L, Lawrence D, Padmanabhan S, Takeshita K, Porter CW, Goodrich DW, Bernstein ZP, Wallace P, Spaner D, Mohr A, Byrne C, Hernandez-Ilizaliturri F, Chrystal C, Starostik P, Czuczman MS (2006) Clinical efficacy of lenalidomide in patients with relapsed or refractory chronic lymphocytic leukemia: results of a phase II study. J Clin Oncol 24:5343–5349

    Article  CAS  PubMed  Google Scholar 

  • Dispenzieri A, Lacy MQ, Zeldenrust SR (2007) The activity of lenalidomide with or without dexamethasone in patients with primary systemic amyloidosis. Blood 109:465–470

    Article  CAS  PubMed  Google Scholar 

  • Kastritis E, Dimopoulos MA (2007) The evolving role of lenalidomide in the treatment of hematologic malignancies. Expert Opin Pharmacother 8:497–509

    Article  CAS  PubMed  Google Scholar 

  • List A, Kurtin S, Roe DJ (2005) Efficacy of lenalidomide in myelodysplastic syndromes. N Engl J Med 352:549–557

    Article  CAS  PubMed  Google Scholar 

  • List A, Dewald G, Bennett J (2006) Lenalidomide in the myelodysplastic syndrome with chromosome 5q deletion. N Engl J Med 355:1456–1465

    Article  CAS  PubMed  Google Scholar 

  • Mitsiades CS, Mitsiades N (2004) CC-5013 (Celgene). Curr Opin Investig Drugs 17:635–647

    Google Scholar 

  • Nianhang C, Lian W, Henry L, Sekhar S, Gondi K (2012) Pharmacokinetics, metabolism and excretion of [(14)C]-lenalidomide following oral administration in healthy male subjects. Cancer Chemother Pharmacol 69:789–797

    Article  Google Scholar 

  • Rajkumar SV, Hayman SR, Lacy MQ, Dispenzieri A, Kabat SMGB, Zeldenrust SR, Kumar S, Greipp PR, Fonseca R, Lust JA, Russell SJ, Kyle RA, Witzig TE, Gertz MA (2005) Combination therapy with lenalidomide plus dexamethasone (Rev/Dex) for newly diagnosed myeloma. Blood 106:4050–4053

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Richardson PG, Blood E, Mitsiades CS, Jagannath S, Zeldenrust SR, Alsina M, Schlossman RL, Rajkumar SV, Desikan KR, Hideshima T, Munshi NC, Kelly-Colson K, Doss D, McKenney ML, Gorelik S, Warren D, Freeman A, Rich R, Wu A, Olesnyckyj M, Wride K, Dalton WS, Zeldis J, Knight R, Weller E, Anderson KC (2006) A randomized phase 2 study of lenalidomide therapy for patients with relapsed or relapsed and refractory multiple myeloma. Blood 108:3458–3464

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Robert K, Judah F (2002) Clinical translation of angiogenesis inhibitors. Nat Rev Cancer 2:727–739

    Article  Google Scholar 

  • Tohnya TM, Hwang K, Lepper ER (2004) Determination of CC-5013, an analogue of thalidomide, in human plasma by liquid chromatography-mass spectrometry. J Chromatogr B Anal Technol Biomed Life Sci 811:135–141

    Article  CAS  Google Scholar 

  • Vaishali S, Daniel GW, Michael R, Kathleen TF, Salli F, Jerome BZ, Martha S, David CS (2007) Lenalidomide and dexamethasone in the treatment of AL amyloidosis: results of a phase 2 trials. Blood 109:492–496

    Article  Google Scholar 

  • Wiernik PH, Lossos IS, Tuscano JM, Justice G, Vose JM, Cole CE, Lam W, McBride K, Wride K, Pietronigro D, Takeshita K, Ervin-Haynes A, Zeldis JB, Habermann TM (2008) Lenalidomide monotherapy in relapsed or refractory aggressive non-Hodgkin’s lymphoma. J Clin Oncol 26:4952–4957

    Article  PubMed  Google Scholar 

  • Witzig TE, Vose J, Pietronigro D (2007) J Clin Oncology 18:445–457

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. KVSR Siddhartha College of Pharmaceutical Sciences, Pinnamaneni Polyclinic Road, Vijayawada, 520010, Andhra Pradesh, India

    Krishna Pocha & G. Devala Rao

Authors
  1. Krishna Pocha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Devala Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Krishna Pocha.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pocha, K., Devala Rao, G. Rapid determination of lenalidomide in rat plasma by an ultra performance liquid chromatography/tandem mass spectrometric method. Med Chem Res 24, 459–467 (2015). https://doi.org/10.1007/s00044-014-1106-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00044-014-1106-x

Keywords

Search

Navigation