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Insulin-like growth factor-6 (IGFBP-6) stimulates neutrophil oxidative burst, degranulation and chemotaxis

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Abstract

The aim of this study was to understand whether insulin-like growth factor-binding protein-6 (IGFBP-6) has functional effects on neutrophils, in particular when they cross epithelium during inflammation. We found that IGFBP-6 increased ROS production (cytofluorimetry), degranulation of primary and tertiary granules (ELISA) and transmigration through the epithelial monolayer. No priming by IGFBP-6 on neutrophils stimulated by either PMA or fMLP was observed. IGFBP-6 is an agonist of neutrophils’ functions, most likely when these cells have been already activated by other stimuli.

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Acknowledgements

This work was in part funded by Associazione Italiana contro le Leucemie-Linfomi e Mieloma (Section of Foggia) (A. Liso). S. Castellani is a researcher funded by Intervento Cofinanziato dal Fondo di Sviluppo e Coesione 2007–2013—APQ Ricerca Regione Puglia “Programma regionale a sostegno della specializzazione intelligente e delle sostenibilità sociale ed ambientali—Future In Research”.

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Authors and Affiliations

  1. Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy

    Massimo Conese, Stefano Castellani, Rosa Trotta & Arcangelo Liso

  2. Section of General Pathology, Department of Biomedical Sciences and Human Oncology, University of Bari, Bari, Italy

    Susanna D’Oria & Pasqualina Montemurro

Authors
  1. Massimo Conese
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  2. Susanna D’Oria
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  3. Stefano Castellani
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  4. Rosa Trotta
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  5. Pasqualina Montemurro
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  6. Arcangelo Liso
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Corresponding authors

Correspondence to Massimo Conese or Arcangelo Liso.

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Responsible Editor: John Di Battista.

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Conese, M., D’Oria, S., Castellani, S. et al. Insulin-like growth factor-6 (IGFBP-6) stimulates neutrophil oxidative burst, degranulation and chemotaxis. Inflamm. Res. 67, 107–109 (2018). https://doi.org/10.1007/s00011-017-1107-6

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  • DOI: https://doi.org/10.1007/s00011-017-1107-6

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