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Malvidin attenuates pain and inflammation in rats with osteoarthritis by suppressing NF-κB signaling pathway

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Abstract

Objective

Malvidin is one of the most widespread anthocyanidins which exhibits significant antioxidant and anti-inflammatory activity. The aim of this paper is to investigate the effects of Malvidin on osteoarthritis (OA).

Materials and methods

We created an animal model of OA using Wistar rats administered by monosodium iodoacetate (MIA). Effects of Malvidin on hyperalgesia were evaluated by paw pressure tests and compression threshold test. Articular chondrocytes were isolated from the OA rats to detect the apoptotic chondrocytes using senescence-associated β-galactosidase (SA-β-gal) staining kit. The expression levels of pro-inflammatory cytokines and matrix metalloproteinase (MMPs) were assessed by western blot and qPCR. Luciferase assay was used to determine the impact of Malvidin on nuclear factor-kappa B (NF-κB) pathway.

Results

Malvidin treatment exhibited significant pain-relieving effects in OA rats and decreased the expression level of apoptotic marker SA-β-gal in chondrocytes. We found that the upregulated expressions of interleukin (IL)-1β, IL-6, tumor necrosis factor-α (TNF-α), and MMPs induced by MIA in cartilage tissues were significantly reversed by Malvidin. Furthermore, Malvidin inhibited NF-κB pathway via an NF-κB inhibitor (IκBα)-independent manner through suppressing p65 nuclear transportation in vitro.

Conclusions

Our findings suggest that Malvidin significantly attenuates the OA-induced pain and inflammation by inhibiting NF-κB signaling pathway and suppressing pro-inflammatory cytokine expression and chondrocyte apoptosis.

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Authors and Affiliations

  1. Department of Orthopedics, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi, 214002, Jiangsu, China

    Teng Dai, Keqing Shi, Gang Chen, Yimin Shen & Ting Pan

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  1. Teng Dai
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  2. Keqing Shi
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  3. Gang Chen
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Correspondence to Ting Pan.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Responsible Editor: John Di Battista.

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Dai, T., Shi, K., Chen, G. et al. Malvidin attenuates pain and inflammation in rats with osteoarthritis by suppressing NF-κB signaling pathway. Inflamm. Res. 66, 1075–1084 (2017). https://doi.org/10.1007/s00011-017-1087-6

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  • DOI: https://doi.org/10.1007/s00011-017-1087-6

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