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Midkine in vitamin D deficiency and its association with anti-Saccharomyces cerevisiae antibodies

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Abstract

Objectives and design

The growth factor midkine (MK) is a protein that is involved in cancer, inflammation, immunity. Vitamin D is a potent immunomodulator. Anti-Saccharomyces cerevisiae antibody (ASCA) is reported in autoimmune disorders, some of which are among the causes of vitamin D deficiency. The objective of this study was to investigate a possible association of MK and ASCA with vitamin D deficiency.

Materials and methods

208 adults presented to internal medicine outpatient clinic for history and physical examination has been studied. Serum biochemistry, vitamin D, MK, ASCA-IgG and -IgA, IL-1β, IL-6, IL-8, TNF-α, PDGF, VEGF were obtained.

Results

Vitamin D deficiency was 74.2 %. Serum MK level was significantly higher in vitamin D-deficient compared to vitamin D-sufficient individuals (1138.1 ± 262.8 vs 958.6 ± 189 pg/mL, respectively; P < 0.009). Serum MK levels were also significantly higher in both ASCA-IgG and -IgA positives compared to negatives (1318.5 ± 160.3 vs 1065.5 ± 256.1, P = 0.008 and 1347.7 ± 229.7 vs 1070.1 ± 250.9 pg/mL, P = 0.011, respectively). Vitamin D was significantly lower in ASCA positives (P = 0.044).Vitamin D showed positive correlation with IL-1β (r 0.338, P < 0.009) and negative correlation with VEGF (r −0.366, P < 0.004).

Conclusions

MK was significantly elevated in vitamin D deficiency and associated with ASCA positivity which was significantly increased in vitamin D deficiency. These findings suggested that molecular mechanism of vitamin D deficiency may be related with some inflammatory processes.

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Acknowledgments

We would like to thank Duzen Laboratories Group, Mecidiyekoy, Istanbul, Turkey, for serum 25-OH D3 measurements by mass spectrometry.

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Authors and Affiliations

  1. Department of Biochemistry, Sakarya Üniversitesi Tıp Fakültesi Dekanlığı, Sakarya University School of Medicine, Korucuk Kampüsü, Konuralp Bulvarı No:81/1, 54187, Sakarya, Turkey

    F. B. Serinkan Cinemre

  2. Department of Internal Medicine, Sakarya University School of Medicine, Sakarya, Turkey

    Hakan Cinemre, Cengiz Karacaer, Ahmet Nalbant, Tezcan Kaya & Ali Tamer

  3. Department of Biophysics, Sakarya University School of Medicine, Sakarya, Turkey

    Birsen Aydemir

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  1. F. B. Serinkan Cinemre
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Correspondence to F. B. Serinkan Cinemre.

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There are no conflicts of interest to be declared and no funding has been received for the conduct of this study.

Ethical standards

This study has been ethically approved by Sakarya University School of Medicine Ethical Committee for Clinical Research with the number of 71522473.050.01.04/54, 2013.

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Responsible Editor: John Di Battista.

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Serinkan Cinemre, F.B., Cinemre, H., Karacaer, C. et al. Midkine in vitamin D deficiency and its association with anti-Saccharomyces cerevisiae antibodies. Inflamm. Res. 65, 143–150 (2016). https://doi.org/10.1007/s00011-015-0898-6

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  • DOI: https://doi.org/10.1007/s00011-015-0898-6

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