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On the conspecificity ofAnopheles fluviatilis species S withAnopheles minimus species C

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Abstract

Anopheles fluviatilis andAn. minimus complexes, each comprising of at least three sibling species, are closely related and important malaria vectors in Oriental Region. RecentlyAn. fluviatilis species S, which is a highly efficient malaria vector in India, has been made conspecific withAn. minimus species C (senior synonym) on the basis of homology in 335 base pair nucleotide sequence of D3 domain of 28S ribosomal DNA(rDNA). We examined the conspecificity of these two nominal species by obtaining and analysing the DNA sequences of nuclear ribosomal loci internal transcribed spacer 2 (ITS2) and D2-D3 domain of 28S rDNA (28S-D2/D3) from those ofAn. fluviatilis S andAn. minimus C. We found that the sequences ofAn. fluviatilis S are appreciably different from those ofAn. minimus C with pair-wise distance (Kimura-2-parametre model) of 3.6 and 0.7% for loci ITS2 and 28S-D2/D3, respectively. Pair-wise distance and phylogenetic analyses using ITS2 sequences of members of Minimus and Fluviatilis Complexes revealedthat An. fluviatilis S is distantly related toAn. minimus C as compared to any other members of the Fluviatilis Complex. These findings suggest that the two nominal species,An. fluviatilis S andAn. minimus C, do not merit synonymy. The study also confirms that the reported speciesAn. fluviatilis X is synonym with species S.

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Abbreviations

ITS2:

Internal transcribed spacer 2

ML:

Maximum Likelihood

MP:

Maximum Parsimony

NJ:

Neighbour-Joining

rDNA:

ribosomal DNA

References

  • Beebe N W and Saul A 1995 Discrimination of all members of theAnopheles punctulatus complex by polymerase chain reaction — restriction fragment length polymorphism analysis;Am. J. Trop. Med. Hyg. 53 478–481

    Article  CAS  Google Scholar 

  • Campbell B C, Steffen-Campbell J D and Werren J H 1993 Phylogeny of the Nasonia species complex (Hymenoptera: Pteromalidae) inferred from an internal transcribed spacer (ITS2) and 28S rDNA sequences;Insect Mol. Biol. 3 225–237

    Google Scholar 

  • Chen B, Harbach R E and Butlin R K 2002 Molecular and morphological studies on theAnopheles minimus group of mosquitoes in southern China: taxonomic review, distribution and malaria vector status;Med. Vet. Entomol. 16 253–265

    Article  CAS  Google Scholar 

  • Chen B, Butlin R K, Pedro P M, Wang X Z and Harbach R E 2006 Molecular variation, systematics and distribution of theAnopheles fluviatilis complex in southern Asia;Med. Vet. Entomol. 20 33–43

    Article  CAS  Google Scholar 

  • Christophers S R 1933The fauna of British India, including Ceylon and Burma, Diptera, volume IV, family Culicidae, tribe Anophelini (London: Taylor and Francis, Red Lion Court, Fleet Street)

    Google Scholar 

  • Coen E S, Strachan T and Dover G 1982 Dynamics of concerted evolution of ribosomal DNA and histone gene families in the melanogaster species subgroup of Drosophila;J. Mol. Biol. 158 17–35

    Article  CAS  Google Scholar 

  • Delphin F and Rao V 1957Guide for the identification of the full-grown larvae and imagines of the anophelines of Burma. Union of Burma (Rangoon)

  • Garros C, Koekemoer L L, Kamau L, Awolola T S, VanBortel W, Coetzee M, Coosemans M and Manguin S 2004 Restriction fragment length polymorphism method for the identification of major African and Asian malaria vectors within theAnopheles funestus andAn. minimus groups;Am. J. Trop. Med. Hyg. 70 260–265

    Article  CAS  Google Scholar 

  • Garros C, Harbach R E and Manguin S 2005 Morphological assessment and molecular phylogenetics of the Funestus and Minimus Groups ofAnopheles (Cellia);J. Med. Entomol. 42 522–536

    Article  CAS  Google Scholar 

  • Garros C, Van Bortel W, Trung H D, Coosemans M and Manguin S 2006 Review of the Minimus Complex of Anopheles, main malaria vector in Southeast Asia: from taxonomic issues to vector control strategies;Trop. Med. Int. Health 11 102–114

    Article  CAS  Google Scholar 

  • Harbach R E 2004 The classificationof genus Anopheles (Diptera: Culicidae): a working hypothesis of phylogenetic relationships;Bull. Entomol. Res. 95 537–553

    Article  Google Scholar 

  • Harrison B A 1980 Medical entomology studies — XIII: The Myzomyia Series ofAnopheles (Cellia) in Thailand, with emphasis on intra-interspecific variations (Diptera: Culicidae);Contrib. Am. Entomol. Ins. 17 1–195

    Google Scholar 

  • Jambulingam P, Sahu S S and Manonmani A 2005 Reappearanceof Anopheles minimus in Singhbum hills of East-Central India;Acta Trop. 96 31–35

    Article  CAS  Google Scholar 

  • Knight K L and Stone A 1977A catalog of the mosquitoes of the world (Diptera: Culicidae), Second edition (Thomas Say Foundation, Entomological Society of America) Vol. 6, p 611

  • Kumar S, Tamura K and Nei M 2004 MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment;Brief. Bioinform. 5 150–163

    Article  CAS  Google Scholar 

  • Litvaitis M K, Nunn G, Thomas W K and Kocher T D 1994 A molecular approach for the identification ofMeiofaunal turbellarians (Platyhelminthes, Turbellaria);Mar. Biol. 120 437–442

    Article  CAS  Google Scholar 

  • Manonmani A, Townson H, Adeniran T, Jambulingam P, Sahu S and Vijayakumar T 2001 rDNA-ITS2 polymerase chain reaction assay for the sibling species ofAnopheles fluviatilis;Acta Trop. 78 3–9

    Article  CAS  Google Scholar 

  • Manonmani A, Nanda N, Jambulingam P, Sahu S, Vijayakumar T, Vani J R and Subbarao S K 2003 Comparison of polymerase chain reaction assay and cytotaxonomy for identification of sibling species ofAnopheles fluviatilis (Diptera: Culicidae);Bull. Entomol. Res. 93 169–171

    Article  CAS  Google Scholar 

  • Nanda N, Joshi H, Subbarao S K, Yadav R S, Shukla R P, Dua V K and Sharma V P 1996Anopheles fluviatilis complex: Host feeding patterns of species S, T and U;J. Am. Mosq. Contr. Assoc. 12 147–149

    CAS  Google Scholar 

  • Posada D and Crandall K A 1998 Modeltest: testing the model of DNA substitution;Bioinformatics 14 817–818

    Article  CAS  Google Scholar 

  • Sharma S K, Nanda N, Dua V K, Joshi H, Subbarao S K and Sharma V P 1995 Studies on the bionomics ofAnopheles fluviatilis sensu lato and the sibling species composition in the foothills of Shiwalik range (Uttar Pradesh), India;S.E. Asian J. Trop. Med. Parasitol. 26 566–572

    Google Scholar 

  • Sharpe R G, Harbach R E and Butlin R K 2000 Molecular variation and phylogeny of members of the Minimus Group ofAnopheles subgenusCellia (Diptera: Culicidae);Syst. Entomol. 25 263–272

    Article  Google Scholar 

  • Shukla R P, Sharma S N, Kohli V K, Nanda N, Sharma V P and Subbarao S K 2001 Dynamics of malaria transmission under changing ecological scenario in and around Nanak Matta Dam, Uttaranchal, India.Indian J. Malariol. 38 91–98

    CAS  PubMed  Google Scholar 

  • Singh O P, Chandra D, Nanda N, Raghavendra K, Sunil S, Sharma S K, Dua V K and Subbarao S K 2004a Differentiation of members of theAnopheles fluviatilis species complex by an allele-specific polymerase chain reaction based on 28S ribosomal DNA sequences;Am. J. Trop. Med. Hyg. 70 27–32

    Article  CAS  Google Scholar 

  • Singh O P, Goswami G, Nanda N, Raghavendra K, Chandra D and Subbarao S K 2004b An allele-specific polymerase chain reaction assay for the differentiation of membersof Anopheles culicifacies complex;J. Biosci. 29 275–280

    Article  CAS  Google Scholar 

  • Subbarao S K, Nanda N, Vasantha K, Dua V K, Malhotra M S, Yadav R S and Sharma V P 1994 Cytogenetic evidence for three sibling species inAnopheles fluviatilis (Diptera: Culicidae); Ann.Entomol. Soc. Am. 87 116–121

    Article  Google Scholar 

  • Subbarao S K 1998Anopheles Species Complexes in South East Asia Region (New Delhi: World Health Organization, South East Asia Regional Office) Technical Publication No. 18

    Google Scholar 

  • Swofford D L 2001PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods) Version 4. (Sinauer Associates, Sunderland, Massachusetts, USA)

    Google Scholar 

  • Torres E P, Foley D H and Bryan J H 2006 Molecular systematics of the Philippine malaria vectorAnopheles flavirostris;Med. Vet. Entomol. 20 44–52

    Article  CAS  Google Scholar 

  • VanBortel W, Trung H D, Roelants P, Harbach R E, Backeljau T and Coosemans M 2000 Molecular identificationof Anopheles minimus s.1. beyond distinguishing the members of the species complex;Insect Mol. Biol. 9 335–340

    Article  Google Scholar 

  • Van Bortel W, Trung H D, Manh N D, Roelants P, Verlé P and Coosemans M 1999 Identification of two species within theAnopheles minimus complex in northern Vietnam and their behavioural divergences;Trop. Med. Int. Health 4 257–265

    Article  Google Scholar 

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Authors and Affiliations

  1. National Institute of Malaria Research, 22 Sham Nath Marg, 110 054, Delhi, India

    O. P. Singh, D. Chandra, N. Nanda, S. K. Sharma, Pe Than Htun, T. Adak, S. K. Subbarao & A. P. Dash

  2. National Institute of Malaria Research, Field Station, Sector 5, 769 002, Rourkela, India

    S. K. Sharma

  3. Department of Medical Research, 5 Ziwaka Road, Dagon Township 11191, Yangan, Myanmar

    Pe Than Htun

  4. Indian Council of Medical Research, Ansari Nagar, 110 029, New Delhi, India

    S. K. Subbarao

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  1. O. P. Singh
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  2. D. Chandra
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  3. N. Nanda
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  4. S. K. Sharma
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  5. Pe Than Htun
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  6. T. Adak
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  7. S. K. Subbarao
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  8. A. P. Dash
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Correspondence to O. P. Singh.

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Singh, O.P., Chandra, D., Nanda, N. et al. On the conspecificity ofAnopheles fluviatilis species S withAnopheles minimus species C. J. Biosci. 31, 671–677 (2006). https://doi.org/10.1007/BF02708419

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  • DOI: https://doi.org/10.1007/BF02708419

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