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Effects of citalopram, a synthetic serotonin uptake inhibitor, on indoleamine and catecholamine concentrations in the cerebrospinal fluid of freely moving rats

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Journal of Neural Transmission - Parkinson's Disease and Dementia Section

Summary

We studied changes in the concentrations of 5-hydroxytryptamine (5-HT), other indoleamines, and catecholamines in the cerebrospinal fluid (CSF) of freely-moving rats that had been administered citalopram, ±1-[3-(Dimethylamino)propyl)-1-(4-fluorophenyl)-1, 3-dihydro-5-isobenzofurancarbonitrile hydrobromide), a selective inhibitor of 5-HT uptake. In a microdialysis experiment, the intracerebral extracellular free 5-HT increased significantly, peaking 60 to 90 min after citalopram (30 mg/kg p.o.) was administerd. The 5-HT concentrations in CSF from the cisterna magna increased significantly, reaching a maximum 6 hours after a single dose of citalopram (30 mg/kg p.o.) was given. Six hours after this dose, the CSF 5-HT concentration in the cisterna magna was significantly increased, and the 5-hydroxyindoleacetic acid (5-HIAA) concentration was significantly decreased. There were non-significant changes in the other indoleamines (tryptophan, 5-hydroxytryptophan, and kynurenine) and in the catecholamines (dopamine, homovanillic acid, normetanephrine, and 3-methoxy-4-hydroxyphenethyleneglycol). The 5-HT/tryptophan ratio was correlated significantly with the kynurenine/tryptophan ratio before treatment with citalopram (r=0.81, p=0.051), indicative that there is coordination of the serotonin and kynurenine pathways in normal rats. In the animals posttreatment there was no such correlation, suggesting that the changes in 5-HT are independent of the kynurenine system at least within the 6 hours postreatment. These CSF results appear to reflect selective inhibition of 5-HT uptake in brain tissues by citalopram that is not associated with changes in catecholamines.

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Author notes

  1. H. Tohgi

    Present address: Department of Neurology, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Japan

Authors and Affiliations

  1. Department of Neurology, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Japan

    T. Abe, M. Nakanishi & S. Takahashi

  2. Department of Pharmacology, Central Research Laboratories, Zeria Pharmaceutical Co., Ltd., Saitama, Japan

    H. Furuichi, T. Matsumura, T. Kurimoto, J. Izumi & Y. Ikeda

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  1. H. Tohgi
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  2. T. Abe
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  3. M. Nakanishi
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  4. S. Takahashi
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  5. H. Furuichi
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  6. T. Matsumura
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  7. T. Kurimoto
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  8. J. Izumi
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  9. Y. Ikeda
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Tohgi, H., Abe, T., Nakanishi, M. et al. Effects of citalopram, a synthetic serotonin uptake inhibitor, on indoleamine and catecholamine concentrations in the cerebrospinal fluid of freely moving rats. J Neural Transm Gen Sect 9, 111–119 (1995). https://doi.org/10.1007/BF02259653

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  • DOI: https://doi.org/10.1007/BF02259653

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