Skip to main content

Identification of Toxic Blooms of Cyanobacteria in Marine Water Habitat

  • Chapter
  • First Online:
Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin

Abstract

Cyanobacteria are a diverse group of photosynthetic bacteria found in various environments, including marine habitats. They play a vital role in marine ecosystems, and some species produce bioactive compounds with biotechnological applications. However, cyanobacteria can also produce toxins called cyanotoxins, which can be harmful to human health and the environment. Cyanobacterial blooms that produce these toxins have been responsible for numerous cases of illness and death in humans and animals. Therefore, it is important to monitor and manage cyanobacterial blooms to minimize risks associated with these toxins. This chapter outlines the methods used to identify toxic blooms of cyanobacteria in marine water habitats, including visual inspection, Polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA). The procedures for sample collection, examination under a microscope, DNA amplification using PCR, and detection of specific toxins using ELISA are described. A combination of these methods can provide a comprehensive understanding of the distribution, toxicity, and potential impacts of toxic cyanobacteria blooms on the ecosystem and public health.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Carmichael WW (1992) Cyanobacteria secondary metabolites—the cyanotoxins. J Appl Microbiol 72(6):445–459

    Google Scholar 

  • Carmichael WW, Azevedo SM, An JS, Molica RJ, Jochimsen EM, Lau S et al (2001) Human fatalities from cyanobacteria: chemical and biological evidence for cyanotoxins. Environ Health Perspect 109(7):663–668

    Article  PubMed  PubMed Central  Google Scholar 

  • Centers for Disease Control and Prevention (2017) Harmful Algal Blooms (HABs). https://www.cdc.gov/habs/index.html

  • Department of Environment and Science (2018) Water sampling and analysis—field manual. https://www.des.qld.gov.au/__data/assets/pdf_file/0025/107404/water-sampling-analysis-field-manual.pdf

  • Environmental Protection Agency (2018) Cyanobacterial harmful algal blooms (CyanoHABs) in water. https://www.epa.gov/nutrientpollution/cyanobacterial-harmful-algal-blooms-cyanohabs-water

  • Falconer IR (2005) Cyanobacterial toxins of drinking water supplies: cylindrospermopsins and microcystins. CRC Press, Boca Raton

    Google Scholar 

  • Glibert PM, Anderson DM (2016) Global ecology and oceanography of harmful algal blooms. In: Ecology of harmful algae. Springer, Cham, pp 119–133

    Google Scholar 

  • Kurmayer R, Kutzenberger T (2003) Application of real-time PCR for quantification of microcystin genotypes in a population of the toxic cyanobacterium Microcystis sp. Appl Environ Microbiol 69(12):6723

    Article  PubMed  PubMed Central  Google Scholar 

  • Lawton LA, Edwards C (2015) Enzyme-linked immunosorbent assays (ELISAs) for detecting and quantifying cyanotoxins in water. In: Cyanobacterial harmful algal blooms: state of the science and research needs. Springer, pp 327–338

    Google Scholar 

  • Meriluoto JA, Spoof L (2017) ELISA

    Google Scholar 

  • Nienaber MA, Steinitz-Kannan M (2018) A guide to cyanobacteria: identification and impact. University Press of Kentucky

    Google Scholar 

  • Paerl HW, Otten TG (2013) Harmful cyanobacterial blooms: causes, consequences, and controls. Microb Ecol 65(4):995–1010

    Article  PubMed  Google Scholar 

  • Paerl HW, Fulton RS, Moisander PH, Dyble J (2001) Harmful freshwater and marine algal blooms. In: Wehr JD, Sheath RG (eds) Freshwater algae of North America: ecology and classification. Academic, San Diego, pp 1091–1138

    Google Scholar 

  • Partensky F, Hess WR, Vaulot D (1999) Prochlorococcus, a marine photosynthetic prokaryote of global significance. Microbiol Mol Biol Rev 63(1):106–127

    Article  PubMed  PubMed Central  Google Scholar 

  • Saker ML, Griffiths DJ (1999) The effect of sample treatment method on PCR detection of microcystin-producing cyanobacteria in water. J Appl Microbiol 87(6):706–715. https://doi.org/10.1046/j.1365-2672.1999.00844.x

    Article  Google Scholar 

  • Saker ML, Griffiths DJ, Falconer IR (2005) Toxins produced by cyanobacteria. In: Water toxins. Springer, pp 1–332

    Google Scholar 

  • Sánchez-Baracaldo P (2015) Origin of marine planktonic cyanobacteria. Science 350(6257):aac7375

    Google Scholar 

  • Scanlan DJ, Ostrowski M, Mazard S, Dufresne A, Garczarek L, Hess WR et al (2009) Ecological genomics of marine picocyanobacteria. Microbiol Mol Biol Rev 73(2):249–299

    Article  PubMed  PubMed Central  Google Scholar 

  • Singh P, Gupta R, Kumari P (2021) Biotechnological potential of cyanobacteria. In: Cyanobacteria. Springer, Singapore, pp 257–282

    Google Scholar 

  • Soto-Liebe K, Fuentes-Valdés JJ, Guerrero MA (2010) PCR detection methods for the identification of potentially toxic cyanobacteria in water and environmental samples. J Appl Microbiol 109(5):1575–1587

    Google Scholar 

  • United States Environmental Protection Agency (EPA) (2019) Standard operating procedure for the collection and analysis of cyanobacterial samples from freshwater and marine environments. EPA/600/R-19/100. https://cfpub.epa.gov/si/si_public_file_download.cfm?p_download_id=540979&Lab=NRMRL

  • Whitton BA, Potts M (2000) The ecology of cyanobacteria: their diversity in time and space. Springer Science & Business Media

    Google Scholar 

  • World Health Organization (WHO) (1999) Identification and quantification of microcystins (cyanobacterial toxins) in water samples. WHO/SDE/WSH/03.04/28. https://www.who.int/water_sanitation_health/dwq/iwachap7.pdf

  • Zehr JP, Mellon MT, Hiorns WD (2001) Phylogeny of cyanobacterial nifH genes: evolutionary implications and potential applications to natural assemblages. Microbiol Mol Biol Rev 65(1):13–37

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2023 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Aloo, S.O., Aloo, D.O., Vijayalakshmi, S., Oh, DH. (2023). Identification of Toxic Blooms of Cyanobacteria in Marine Water Habitat. In: Thajuddin, N., Sankara narayanan, A., Dhanasekaran, D. (eds) Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin . Springer, Singapore. https://doi.org/10.1007/978-981-99-4514-6_3

Download citation

Publish with us

Policies and ethics