Abstract
In order to explore the bioelectric effect of high-voltage extremely short pulse electric fields (esPEFs) on tumor cells, first, based on the finite element platform, a spherical single-cell Multiphysics model was established. Both electroporation and dielectric relaxation of the membrane are considered. The electroporation effects and Maxwell stress tensors on cell and nuclear membranes were analyzed; secondly, taking MDA-MB-231 cells as the research object, combined with fluorescent probe technology, The state change and fluorescence dissipation of its subcellular structure exposed to esPEFs were studied. The results show that high-pressure esPEFs preferentially act inside the cell, causing electroporation effect and tensile stress on the nuclear membrane; it destroys the integrity and order of the cytoskeleton, and damage the subcellular structural functions of the endoplasmic reticulum, mitochondria, and nucleus. The degree of damage is positively correlated with the pulse dose.
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This work is supported by National Natural Science Foundation of China (NSFC) under grant No. 51877022.
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Ma, X., Wang, Y., Chen, Y., Dong, S., Yao, C. (2023). Simulation and Experimental Study on Damage of Subcellular Structure by High Voltage and Extremely Short Pulse Electric Field. In: Yang, Q., Dong, X., Ma, W. (eds) The proceedings of the 10th Frontier Academic Forum of Electrical Engineering (FAFEE2022). FAFEE 2022. Lecture Notes in Electrical Engineering, vol 1048. Springer, Singapore. https://doi.org/10.1007/978-981-99-3404-1_77
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DOI: https://doi.org/10.1007/978-981-99-3404-1_77
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