Skip to main content

Classification of Microorganisms from Sparsely Limited Data Using a Proposed Deep Learning Ensemble

  • Conference paper
  • First Online:
Inventive Systems and Control

Part of the book series: Lecture Notes in Networks and Systems ((LNNS,volume 672))

  • 228 Accesses

Abstract

With the recent advancements in medicine and biotechnology, there is always a growing need for more sophisticated decision systems to aid in certain biological factors, such as detecting a pathogen or microscopic organism and its accurate classification. In such a domain, there is very little tolerance for error, and the highest priority must be given to creating intelligent systems capable of doing so. Advancements in computer vision and other state-of-the-art image processing techniques enable computation systems to extract and analyze principal features from a specimen that even a trained eye may otherwise miss. There has also been an upsurge in the research on the applications of machine learning to aid in specific surgical procedures and diagnostic tests. Classifying organisms at the micro-scale is an essential stepping stone for their long-term success. The study aims to ensembling the best machine learning techniques and models in the computer vision domain, such as transfer learning, top-performing models, and image preprocessing methods, to maximize the capabilities of a typical image classification model for a sparse and limited multi-labeled class dataset containing microscopic images and hence shed light and explore into how more excellent performance can be obtained while facing classical dataset limitations.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 219.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 279.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Li Z, Li C, Yao Y, Zhang J, Rahaman MM, Xu H et al (2021) Environmental microorganism image dataset fifth version for multiple image analysis tasks. PLoS ONE 16(5):99–110. https://doi.org/10.10007/1234567890

  2. Waquar (2022) Micro-organism image classification. Kaggle. https://doi.org/10.34740/KAGGLE/DSV/4032122

  3. Poomrittigul S, Chomkwah W, Tanpatanan T, Sakorntanant S, Treebupachatsakul T (2022) A comparison of deep learning CNN architecture models for classifying bacteria. In: 2022 37th International technical conference on circuits/systems, computers and communications (ITC-CSCC), pp 290–293. https://doi.org/10.1109/ITC-CSCC55581.2022.9894986

  4. Chen W, Liu P, Lai C, Lin Y (2022) Identification of environmental microorganism using optimally fine-tuned convolutional neural network. Environ Res 206:112610. ISSN 0013-9351, https://doi.org/10.1016/j.envres.2021.112610

  5. Kim HE, Maros ME, Siegel F, Ganslandt T (2022) Rapid convolutional neural networks for gram-stained image classification at inference time on mobile devices: empirical study from transfer learning to optimization. Biomedicines 10:2808. https://doi.org/10.3390/biomedicines10112808

    Article  Google Scholar 

  6. Uma Venkata Ravi Teja K, Pavan Venkat Reddy B, Likith Preetham A, Patil HY (2021) Poorna Chandra T (2021) Prediction of diabetes at early stage with supplementary polynomial features. In: Smart technologies, communication and robotics (STCR), pp 1–5. https://doi.org/10.1109/STCR51658.2021.9588849

  7. Uma Venkata Ravi Teja K, Pavan Venkat Reddy B, Alla LP, Patil HY (2021) Parkinson’s disease classification using quantile transformation and RFE. In: 2021 12th International conference on computing communication and networking technologies (ICCCNT), pp 01–05. https://doi.org/10.1109/ICCCNT51525.2021.9580024

  8. Hebbar N, Patil HY, Agarwal K (2020) Web powered CT scan diagnosis for brain hemorrhage using deep learning. In: 2020 IEEE 4th Conference on information & communication technology (CICT), pp 1–5. https://doi.org/10.1109/CICT51604.2020.9312098

  9. Patil P, Patil H (2020) X-ray imagining based pneumonia classification using deep learning and adaptive clip limit based CLAHE algorithm. In: 2020 IEEE 4th Conference on information & communication technology (CICT), pp 1–4. https://doi.org/10.1109/CICT51604.2020.9312089

  10. Wang F, Ma M, Cao H, Chai X, Huang M, Liu L (2022) Conjugated polymer materials for detection and discrimination of pathogenic microorganisms: guarantee of biosafety. Biosaf Health 4(2):79–86. ISSN 2590-0536, https://doi.org/10.1016/j.bsheal.2022.03.006

  11. Ma P, Li C, Rahaman MM et al (2022) A state-of-the-art survey of object detection techniques in microorganism image analysis: from classical methods to deep learning approaches. Artif Intell Rev. https://doi.org/10.1007/s10462-022-10209-1

    Article  Google Scholar 

  12. Kulwa F, Li C, Grzegorzek M, Rahaman M, Shirahama K, Kosov S (2023) Segmentation of weakly visible environmental microorganism images using pair-wise deep learning features. Biomed Signal Process Control 79(Part 2):104168. ISSN 1746–8094. https://doi.org/10.1016/j.bspc.2022.104168

  13. Zhang J, Ma P, Jiang T, Zhao X, Tan W, Zhang J, Zou S, Huang X, Grzegorzek M, Li C (2022) SEM-RCNN: a squeeze-and-excitation-based mask region convolutional neural network for multi-class environmental microorganism detection. Appl Sci 12:9902. https://doi.org/10.3390/app12199902

    Article  Google Scholar 

  14. Prada P, Brunel B, Reffuveille F, Gangloff SC (2022) Technique evolutions for microorganism detection in complex samples: a review. Appl Sci 12:5892. https://doi.org/10.3390/app12125892

    Article  Google Scholar 

  15. Shao R, Bi X-J, Chen Z (2022) A novel hybrid transformer-CNN architecture for environmental microorganism classification. PLoS ONE 17(11):e0277557. https://doi.org/10.1371/journal.pone.0277557

    Article  Google Scholar 

  16. Zhang J, Li C, Yin Y et al (2022) Applications of artificial neural networks in microorganism image analysis: a comprehensive review from conventional multilayer perceptron to popular convolutional neural network and potential visual transformer. Artif Intell Rev. https://doi.org/10.1007/s10462-022-10192-7

    Article  Google Scholar 

  17. Rani P, Kotwal S, Manhas J et al (2022) Machine learning and deep learning based computational approaches in automatic microorganisms image recognition: methodologies, challenges, and developments. Arch Comput Methods Eng 29:1801–1837. https://doi.org/10.1007/s11831-021-09639-x

    Article  Google Scholar 

  18. Kulwa F et al (2019) A state-of-the-art survey for microorganism image segmentation methods and future potential. IEEE Access 7:100243–100269. https://doi.org/10.1109/ACCESS.2019.2930111

    Article  Google Scholar 

  19. Narain Ponraj D et al (2011) A survey on the preprocessing techniques of mammogram for the detection of breast cancer. J Emerg Trends Comput Inf Sci 2(12):656–664

    Google Scholar 

  20. Chao D, Loy Change C, Kaiming H, Xiaoou T (2014) Image super-resolution using deep convolutional networks. IEEE Trans Pattern Anal Mach Intell 38. https://doi.org/10.1109/TPAMI.2015.2439281

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Gautam Chettiar .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2023 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

About this paper

Check for updates. Verify currency and authenticity via CrossMark

Cite this paper

Chettiar, G., Shukla, A., Patil, H., Jindal, S. (2023). Classification of Microorganisms from Sparsely Limited Data Using a Proposed Deep Learning Ensemble. In: Suma, V., Lorenz, P., Baig, Z. (eds) Inventive Systems and Control. Lecture Notes in Networks and Systems, vol 672. Springer, Singapore. https://doi.org/10.1007/978-981-99-1624-5_22

Download citation

Publish with us

Policies and ethics