Abstract
This paper reports the use of tunable diode laser absorption spectroscopy (TDLAS) to measure the carbon dioxide (CO2) in the culture vials. The accumulated CO2 concentration in the headspace is linked with the growth of the microorganisms cultured in the vials. The 2004.1 nm spectral line of CO2 is selected as the sensing transition for its strong absorption strength. The proposed TDLAS sensor is calibrated and verified with several CO2 standard gases, yielding a minimum 1σ detection limit of 0.084% with an integral time of 3 s. The suitability of the sensor to the microorganisms is performed, almost the results agree with the growth laws of microbiology. Since the sensor is fast and nondestructive, it can be promising for in-line detection of microbial growth.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Danilović B, Savić D, Cocola L, Fedel M, Poletto L (2018) Determination of CO2 content in the headspace of spoiled yogurt packages. J Food Qual 8121606
Moriaux A, Vallon R, Parvitte B, Zeninari V, Liger-Belair G, Cilindre C (2018) Monitoring gas-phase CO2 in the headspace of champagne glasses through combined diode laser spectrometry and micro-gas chromatography analysis. Food Chem 264:255–262
Hu P, Pan S, Chen Z, Guo G, Zhang J, Li G, Chen N (2017) Hydrogen peroxide detection based on near-infrared TDLAS and Herriott cell. In: 16th international conference on optical communications and networks (ICOCN). Wuzhen, China, pp 1–3
Yang H, Chen J, Luo X, Liu C, Qi D, Xin X (2019) Leakage detection of closed vials based on two-line water-vapor TDLAS. Measurement 135:413–417
Cai T, Wang G, Cao Z, Zhang W, Gao X (2014) Sensor for headspace pressure and H2O concentration measurements in closed vials by tunable diode laser absorption spectroscopy. Opt Lasers Eng 58:48–53
Cocola L, Fedel M, Giuseppe Tondello G, Frazzi G, Poletto L, Bardenshtein A (2018) Design and evaluation of an in-line system for gas sensing in flow-packed products. Food Packag Shelf Life 17:91–98
Brueckner D, Roesti D, Zuber UG, Schmidt R, Kraehenbuehl S, Bonkat G (2016) Comparison of tunable diode laser absorption spectroscopy and isothermal micro-calorimetry for non-invasive detection of microbial growth in media fills. Sci Rep 6(1):27894
Peng Z, Ding Y, Chen L, Li X, Zheng K (2011) Calibration-free wavelength modulated tdlas under high absorbance conditions. Opt Express 19(23):23104
Cocola L, Allermann H, Fedel M, Sonderby S, Tondello G, Bardenstein A (2016) Validation of an in-line non-destructive headspace oxygen sensor. Food Packag Shelf Life 9:38–44
Zarin AS, Chakraborty AL (2015) Absolute concentration measurements of bacterial CO2 emission using a 2004 nm vertical cavity surface emitting tunable diode laser. Workshop on recent advances in photonics (WRAP), Bangalore, India
Acknowledgements
This work was supported by National Natural Science Foundation of China under grant Nos. 41605101 and 41805108.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
Chen, Z., Hu, P., Pan, S., Zhang, J., Guo, G., Liu, S. (2021). Tunable Diode Laser Absorption Spectroscopy for Non-invasive Detection of Microbial Growth. In: Peng, Y., Dong, X. (eds) Proceedings of 2019 International Conference on Optoelectronics and Measurement. Lecture Notes in Electrical Engineering, vol 726. Springer, Singapore. https://doi.org/10.1007/978-981-33-4110-4_22
Download citation
DOI: https://doi.org/10.1007/978-981-33-4110-4_22
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-33-4109-8
Online ISBN: 978-981-33-4110-4
eBook Packages: EngineeringEngineering (R0)