Abstract
A combination of bioactive scaffold with drug delivery of therapeutic agents is a great deal to locally treat bone infections. However, controllable drug release behaviours for different types of drug-incorporated scaffold have been not comprehensively compared. In this study, novel technique was proposed with the addition of bioactive agents in microspheres was incorporated into carbonate apatite (CO3Ap) scaffold. A simple slurry-dipping method by dispersion of 0.8 wt% suspension of gentamicin (GEN)-loaded polylactic acid (PLA) microsphere (GENMS) using an ultrasonic bath was used to coat the scaffold. GENMS was fabricated by double emulsion. This coated scaffold was compared to the GEN coated scaffold without microsphere and uncoated scaffold with direct loading of GEN. It was confirmed that the microsphere coating did not inhibit the apatite growth of the scaffold when immersed in Hank’s Balance Salt Solution for 4 weeks. The drug release profile exhibited the initial burst and sustained drug release could be improved by the presence of GENMS in the coated scaffold. Moreover, the kinetic release study supported the findings of different drug release based on zero-order, first-order, Higuchi and Korsmeyer–Peppas models. The results showed that drug release mechanisms were diffusion and degradation controlled for scaffold, while for coated scaffolds led to diffusion and degradation of chitosan and microsphere. Rougher surface of the scaffold by the adhered GENMS on the scaffold facilitated cell proliferation. In short, this multifunctional coated bioactive scaffold has the potential to enhance cell attachment and provide local of controlled drug delivery for bone tissue engineering improvement.
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Mamat, N., Mariatti, M., Hamid, Z.A.A., Yahaya, B.H. (2021). Enhancement of Local Drug Delivery System Using Different Design of Gentamicin Loaded in Carbonate Apatite Scaffold. In: Hameed Sultan, M.T., Majid, M.S.A., Jamir, M.R.M., Azmi, A.I., Saba, N. (eds) Biocomposite Materials. Composites Science and Technology . Springer, Singapore. https://doi.org/10.1007/978-981-33-4091-6_12
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