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Nanoenzyme-Based Electrodes in Biomolecular Screening and Analysis

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Handbook of Nanobioelectrochemistry

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Abstract

Nanobioelectrochemistry is a multidiscipline branch of science encompassing nanomaterials, biotechnology, and electrochemistry. In principle, nanomaterials with enzyme-like properties, simply referred to as nanozymes, are used in place of natural and traditional artificial enzymes due to their resilience under a wide range of stringent conditions. Additional advantages of nanozymes include extended storage times, cost-effectiveness, and enhanced catalytic properties. Mechanisms of action make use of redox activities to generate a signal that can be used to provide insight on various analytic procedures. Some areas of major application include but are not limited to disease detection and control, in addition to food safety. Herein, we focus on current trends in the use of nanozymes in bioelectrochemistry. Precisely, we highlight how electrodes in nano-bioelectrochemistry may be fabricated for optimum performance. Furthermore, we focus on biosensing techniques, food monitoring, cancer diagnosis, cancer therapy, bacteria detection, and Covid-19 detection as part of current trends in nanozymes in nano-electrochemistry.

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References

  • Arcus VL, Prentice EJ, Hobbs JK, Mulholland AJ, Van Der Kamp MW, Pudney CR, Parker EJ, Schipper LA (2016) On the temperature dependence of enzyme-catalyzed rates. Biochemistry 55(12):1681–1688

    Article  CAS  PubMed  Google Scholar 

  • Bage N, Kar P (2021) Ultrasensitive electrochemical sensing of biotin-Avidin interaction on gold electrode bio-conjugated with silver nanoparticles. IEEE Sensors J 21(9):10400–10408

    Article  CAS  Google Scholar 

  • Blanco A, Blanco G (2017) Medical biochemistry. Academic Press

    Google Scholar 

  • Bornscheuer UT, Huisman G, Kazlauskas R, Lutz S, Moore J, Robins K (2012) Engineering the third wave of biocatalysis. Nature 485(7397):185–194

    Article  CAS  PubMed  Google Scholar 

  • Breslow R, Overman LE (1970) " artificial enzyme" combining a metal catalytic group and a hydrophobic binding cavity. J Am Chem Soc 92(4):1075–1077

    Article  CAS  PubMed  Google Scholar 

  • Brynskikh AM, Zhao Y, Mosley RL, Li S, Boska MD, Klyachko NL, Kabanov AV, Gendelman HE, Batrakova EV (2010) Macrophage delivery of therapeutic nanozymes in a murine model of Parkinson’s disease. Nanomedicine 5(3):379–396

    Article  CAS  PubMed  Google Scholar 

  • Cavalcante FTT, de A Falcão IR, da S Souza JE, Rocha TG, De Sousa IG, Cavalcante ALG, De Oliveira ALB, De Sousa MCM, Dos Santos JCS (2021) Designing of nanomaterials-based enzymatic biosensors: synthesis, properties, and applications. Electrochemistry 2(1):149–184

    CAS  Google Scholar 

  • Chatterjee B, Das SJ, Anand A, Sharma TK (2020) Nanozymes and aptamer-based biosensing. Mater Sci Technol 3:127–135

    CAS  Google Scholar 

  • Cheng H, Zhang L, He J, Guo W, Zhou Z, Zhang X, Nie S, Wei H (2016) Integrated nanozymes with nanoscale proximity for in vivo neurochemical monitoring in living brains. Anal Chem 88(10):5489–5497

    Article  CAS  PubMed  Google Scholar 

  • Cheng N, Song Y, Zeinhom MM, Chang Y-C, Sheng L, Li H, Du D, Li L, Zhu M-J, Luo Y (2017) Nanozyme-mediated dual immunoassay integrated with smartphone for use in simultaneous detection of pathogens. ACS Appl Mater Interfaces 9(46):40671–40680

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cooper GM (2000) The central role of enzymes as biological catalysts. Sinauer Associates

    Google Scholar 

  • Das B, Franco JL, Logan N, Balasubramanian P, Kim MI, Cao C (2021) Nanozymes in point-of-care diagnosis: an emerging futuristic approach for biosensing. Nano-Micro Lett 13(1):1–51

    Article  CAS  Google Scholar 

  • Dos Santos GP, Da Silva BF, Garrido SS, Mascini M, Yamanaka H (2014) Design, synthesis and characterization of a hexapeptide bio-inspired by acetylcholinesterase and its interaction with pesticide dichlorvos. Analyst 139(1):273–279

    Article  PubMed  Google Scholar 

  • Feng L, Dong Z, Liang C, Chen M, Tao D, Cheng L, Yang K, Liu Z (2018) Iridium nanocrystals encapsulated liposomes as near-infrared light controllable nanozymes for enhanced cancer radiotherapy. Biomaterials 181:81–91

    Article  CAS  PubMed  Google Scholar 

  • Feng H, Bao M, Lian M, Li G (2021. IOP Publishing) Glypican-3 electrochemical aptasensor based on CuO-rGO nanocomposite. J Phys Conf Ser 2009:012071

    Article  CAS  Google Scholar 

  • Ferrag C, Kerman K (2020) Grand challenges in nanomaterial-based electrochemical sensors. Front Sens 1(5):1–4. https://doi.org/10.3389/fsens.2020.583822

  • Gao L, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N, Wang T, Feng J, Yang D, Perrett S (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2(9):577–583

    Article  CAS  PubMed  Google Scholar 

  • Gao L, Fan K, Yan X (2020) Iron oxide nanozyme: a multifunctional enzyme mimetics for biomedical application. Nano:105. https://doi.org/10.1007/978-981-15-1490-6_5

  • Goswami N, Giri A, Bootharaju M, Xavier PL, Pradeep T, Pal SK (2011) Copper quantum clusters in protein matrix: potential sensor of Pb2+ ion. Anal Chem 83(24):9676–9680

    Article  CAS  PubMed  Google Scholar 

  • Gugoasa LAD, Pogacean F, Kurbanoglu S, Barbu-Tudoran L, Serban A, Kacso I, Pruneanu S (2021) Graphene-gold nanoparticles Nanozyme-based electrochemical sensor with enhanced laccase-like activity for determination of phenolic substrates. J Electrochem Soc 168:067523

    Article  CAS  Google Scholar 

  • Guo LJ (2004) Recent progress in nanoimprint technology and its applications. J Phys D Appl Phys 37(11):R123

    Article  CAS  Google Scholar 

  • Han J, Zhang L, Hu L, Xing K, Lu X, Huang Y, Zhang J, Lai W, Chen T (2018) Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157: H7 in milk. J Dairy Sci 101(7):5770–5779

    Article  CAS  PubMed  Google Scholar 

  • Hasanzadeh M, Shadjou N (2016) Pharmacogenomic study using bio-and nanobioelectrochemistry: drug–DNA interaction. Mater Sci Eng C 61:1002–1017

    Article  CAS  Google Scholar 

  • Hu M, Korschelt K, Daniel P, Landfester K, Tremel W, Bannwarth MB (2017) Fibrous nanozyme dressings with catalase-like activity for H2O2 reduction to promote wound healing. ACS Appl Mater Interfaces 9(43):38024–38031

    Google Scholar 

  • Huang Y, Ren J, Qu X (2019) Nanozymes: classification, catalytic mechanisms, activity regulation, and applications. Chem Rev 119(6):4357–4412

    Article  CAS  PubMed  Google Scholar 

  • Idili A, Arroyo-Currás N, Ploense KL, Csordas AT, Kuwahara M, Kippin TE, Plaxco KW (2019) Seconds-resolved pharmacokinetic measurements of the chemotherapeutic irinotecan in situ in the living body. Chem Sci 10(35):8164–8170

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jiang L, Gu S, Ding Y, Jiang F, Zhang Z (2014) Facile and novel electrochemical preparation of a graphene–transition metal oxide nanocomposite for ultrasensitive electrochemical sensing of acetaminophen and phenacetin. Nanoscale 6(1):207–214

    Article  CAS  PubMed  Google Scholar 

  • Jiang J, He C, Wang S, Jiang H, Li J, Li L (2018) Recyclable ferromagnetic chitosan nanozyme for decomposing phenol. Carbohydr Polym 198:348–353

    Article  CAS  PubMed  Google Scholar 

  • Jiang D, Ni D, Rosenkrans ZT, Huang P, Yan X, Cai W (2019) Nanozyme: new horizons for responsive biomedical applications. Chem Soc Rev 48(14):3683–3704

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jiang Z, Feng B, Xu J, Qing T, Zhang P, Qing Z (2020) Graphene biosensors for bacterial and viral pathogens. Biosens Bioelectron 166:112471–112471

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Khairy M, Khorshed AA, Rashwan FA, Salah GA, Abdel-Wadood HM, Banks CE (2017) Simultaneous voltammetric determination of antihypertensive drugs nifedipine and atenolol utilizing MgO nanoplatelet modified screen-printed electrodes in pharmaceuticals and human fluids. Sensors Actuators B Chem 252:1045–1054

    Article  CAS  Google Scholar 

  • Khairy M, Ayoub HA, Banks CE (2018) Non-enzymatic electrochemical platform for parathion pesticide sensing based on nanometer-sized nickel oxide modified screen-printed electrodes. Food Chem 255:104–111

    Article  CAS  PubMed  Google Scholar 

  • Kuah E, Toh S, Yee J, Ma Q, Gao Z (2016) Enzyme mimics: advances and applications. Chem Eur J 22(25):8404–8430

    Article  CAS  PubMed  Google Scholar 

  • Kuralay F (2019) Chapter 12 - nanomaterials-based enzyme biosensors for electrochemical applications: recent trends and future prospects. In: Ozkan SA, Shah A (eds) New developments in nanosensors for pharmaceutical analysis. Academic Press

    Google Scholar 

  • Lei C-X, Hu S-Q, Shen G-L, Yu R-Q (2003) Immobilization of horseradish peroxidase to a nano-au monolayer modified chitosan-entrapped carbon paste electrode for the detection of hydrogen peroxide. Talanta 59(5):981–988

    Article  CAS  PubMed  Google Scholar 

  • Lei CX, Wang H, Shen GL, Yu RQ (2004) Immobilization of enzymes on the nano-au film modified glassy carbon electrode for the determination of hydrogen peroxide and glucose. Electroanalysis 16(9):736–740

    Article  CAS  Google Scholar 

  • Li C, Wang C, Wang C, Hu S (2006) Development of a parathion sensor based on molecularly imprinted nano-TiO2 self-assembled film electrode. Sensors Actuators B Chem 117(1):166–171

    Google Scholar 

  • Li T, Li Y, Zhang T (2019) Materials, structures, and functions for flexible and stretchable biomimetic sensors. Acc Chem Res 52(2):288–296

    Article  CAS  PubMed  Google Scholar 

  • Liang C, Liu B, Li J, Lu J, Zhang E, Deng Q, Zhang L, Chen R, Fu Y, Li C (2021) A nanoenzyme linked immunochromatographic sensor for rapid and quantitative detection of SARS-CoV-2 nucleocapsid protein in human blood. Sensors Actuators B Chem 349:130718

    Article  CAS  Google Scholar 

  • Ligler FS, Gooding JJ (2019) Lighting up biosensors: now and the decade to come. Anal Chem 91(14):8732–8738

    Article  CAS  PubMed  Google Scholar 

  • Liu Z-M, Yang Y, Wang H, Liu Y-L, Shen G-L, Yu R-Q (2005) A hydrogen peroxide biosensor based on nano-au/PAMAM dendrimer/cystamine modified gold electrode. Sensors Actuators B Chem 106(1):394–400

    Article  CAS  Google Scholar 

  • Liu D, Ju C, Han C, Shi R, Chen X, Duan D, Yan J, Yan X (2021) Nanozyme chemiluminescence paper test for rapid and sensitive detection of SARS-CoV-2 antigen. Biosens Bioelectron 173:112817

    Article  CAS  Google Scholar 

  • Lu D, Zhang Y, Wang L, Lin S, Wang C, Chen X (2012) Sensitive detection of acetaminophen based on Fe3O4 nanoparticles-coated poly(diallyldimethylammonium chloride)-functionalized graphene nanocomposite film. Talanta 88:181–186

    Google Scholar 

  • Mahapatra S, Baranwal A, Purohit B, Roy S, Mahto SK, Chandra P (2020) Advanced biosensing methodologies for ultrasensitive detection of human coronaviruses. In: Chandra P, Roy S (eds) Diagnostic strategies for COVID-19 and other coronaviruses. Singapore, Springer Singapore

    Google Scholar 

  • Mahato K, Kumar S, Srivastava A, Maurya PK, Singh R, Chandra P (2018) Electrochemical immunosensors: fundamentals and applications in clinical diagnostics. In: Handbook of immunoassay technologies. Elsevier

    Google Scholar 

  • Mahmoud BG, Khairy M, Rashwan FA, Foster CW, Banks CE (2016) Self-assembly of porous copper oxide hierarchical nanostructures for selective determinations of glucose and ascorbic acid. RSC Adv 6(18):14474–14482

    Article  CAS  Google Scholar 

  • Mahmudunnabi RG, Farhana FZ, Kashaninejad N, Firoz SH, Shim Y-B, Shiddiky MJ (2020) Nanozyme-based electrochemical biosensors for disease biomarker detection. Analyst 145(13):4398–4420

    Article  CAS  PubMed  Google Scholar 

  • Manea F, Houillon FB, Pasquato L, Scrimin P (2004) Nanozymes: gold-nanoparticle-based transphosphorylation catalysts. Angew Chem 116(45):6291–6295

    Article  Google Scholar 

  • Mei X, Hu T, Wang H, Liang R, Bu W, Wei M (2020) Highly dispersed nano-enzyme triggered intracellular catalytic reaction toward cancer specific therapy. Biomaterials 258:120257

    Article  CAS  PubMed  Google Scholar 

  • Meng L, Turner AP, Mak WC (2020) Soft and flexible material-based affinity sensors. Biotechnol Adv 39:107398

    Article  CAS  PubMed  Google Scholar 

  • Murphy L (2006) Biosensors and bioelectrochemistry. Curr Opin Chem Biol 10(2):177–184

    Article  CAS  PubMed  Google Scholar 

  • Parham H, Rahbar N (2010) Square wave voltammetric determination of methyl parathion using ZrO2-nanoparticles modified carbon paste electrode. J Hazard Mater 177(1–3):1077–1084

    Article  CAS  PubMed  Google Scholar 

  • Parsaee Z, Karachi N, Abrishamifar SM, Kahkha MRR, Razavi R (2018) Silver-choline chloride modified graphene oxide: novel nano-bioelectrochemical sensor for celecoxib detection and CCD-RSM model. Ultrason Sonochem 45:106–115

    Article  CAS  PubMed  Google Scholar 

  • Peterson ME, Daniel RM, Danson MJ, Eisenthal R (2007) The dependence of enzyme activity on temperature: determination and validation of parameters. Biochem J 402(2):331–337

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Qiu L, Lv P, Zhao C, Feng X, Fang G, Liu J, Wang S (2019) Electrochemical detection of organophosphorus pesticides based on amino acids conjugated nanoenzyme modified electrodes. Sensors Actuators B Chem 286:386–393

    Article  CAS  Google Scholar 

  • Qiu P, Yuan P, Deng Z, Su Z, Bai Y, He J (2021) One-pot facile synthesis of enzyme-encapsulated Zn/co-infinite coordination polymer nanospheres as a biocatalytic cascade platform for colorimetric monitoring of bacteria viability. Microchim Acta 188(10):322

    Article  CAS  Google Scholar 

  • Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, Dhama K (2014) Oxidative stress, prooxidants, and antioxidants: the interplay. Biomed Res Int 2014:761264

    Article  PubMed  PubMed Central  Google Scholar 

  • Ranadheera CS, Prasanna PHP, Vidanarachchi JK, Mcconchie R, Naumovski N, Mellor D (2017) Chapter 12 – Nanotechnology in microbial food safety. In: Oprea AE, Grumezescu AM (eds) Nanotechnology applications in food. Academic Press

    Google Scholar 

  • Ruff A (2017) Redox polymers in bioelectrochemistry: common playgrounds and novel concepts. Curr Opin Electrochem 5(1):66–73

    Article  CAS  Google Scholar 

  • Shanbhag MM, Ilager D, Mahapatra S, Shetti NP, Chandra P (2021) Amberlite XAD-4 based electrochemical sensor for diclofenac detection in urine and commercial tablets. Mater Chem Phys 273:125044

    Article  CAS  Google Scholar 

  • Sims CM, Hanna SK, Heller DA, Horoszko CP, Johnson ME, Montoro Bustos AR, Reipa V, Riley KR, Nelson BC (2017) Redox-active nanomaterials for nanomedicine applications. Nanoscale 9(40):15226–15251

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Singh S (2016) Cerium oxide based nanozymes: redox phenomenon at biointerfaces. Biointerphases 11(4):04B202

    Article  PubMed  Google Scholar 

  • Singh S (2019) Nanomaterials exhibiting enzyme-like properties (Nanozymes): current advances and future perspectives. Front Chem 7:46

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Singh AP, Balayan S, Hooda V, Sarin R, Chauhan N (2020) Nano-interface driven electrochemical sensor for pesticides detection based on the acetylcholinesterase enzyme inhibition. Int J Biol Macromol 164:3943–3952

    Article  CAS  PubMed  Google Scholar 

  • Solanki S, Pandey CM, Gupta RK, Malhotra BD (2021) Chapter 3 – Nanobioelectrochemistry: fundamentals and biosensor applications. In: Wain AJ, Dickinson EJF (eds) Frontiers of nanoscience. Elsevier

    Google Scholar 

  • Su H, Li S, Jin Y, Xian Z, Yang D, Zhou W, Mangaran F, Leung F, Sithamparanathan G, Kerman K (2017) Nanomaterial-based biosensors for biological detections. Adv Health Care Technol 3:19–29

    Article  Google Scholar 

  • Sun H, Zhou Y, Ren J, Qu X (2018) Carbon nanozymes: enzymatic properties, catalytic mechanism, and applications. Angew Chem Int Ed 57(30):9224–9237

    Article  CAS  Google Scholar 

  • Thage RL, Semegni Y, Naidoo S (2020) Computer-aided: modelled sustainable hybrid catalysts for a Nano-drug delivery system. S Afr J Chem 73:103–110

    Google Scholar 

  • Vermisoglou E, Panáček D, Jayaramulu K, Pykal M, Frébort I, Kolář M, Hajdúch M, Zbořil R, Otyepka M (2020) Human virus detection with graphene-based materials. Biosens Bioelectron 166:112436

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Vigneshvar S, Senthilkumaran B (2018) Current technological trends in biosensors, nanoparticle devices and biolabels: hi-tech network sensing applications. Med Dev Sens 1(2):e10011

    Article  Google Scholar 

  • Wang W, Gunasekaran S (2020) Nanozymes-based biosensors for food quality and safety. TrAC Trends Anal Chem 126:115841

    Article  CAS  Google Scholar 

  • Wang L, Miao L, Yang H, Yu J, Xie Y, Xu L, Song Y (2017) A novel nanoenzyme based on Fe3O4 nanoparticles@thionine-imprinted polydopamine for electrochemical biosensing. Sensors Actuators B Chem 253:108–114

    Article  CAS  Google Scholar 

  • Wang H, Wan K, Shi X (2019) Recent advances in nanozyme research. Adv Mater 31(45):1805368

    Article  CAS  Google Scholar 

  • Wang S, Mao J, Liu H, Huang S, Cai J, Gui W, Wu J, Xu J, Shen J, Wang Z (2020) pH-sensitive nanotheranostics for dual-modality imaging guided nanoenzyme catalysis therapy and phototherapy. J Mater Chem B 8(22):4859–4869

    Article  CAS  PubMed  Google Scholar 

  • Wei H, Wang E (2008) Fe3O4 magnetic nanoparticles as peroxidase mimetics and their applications in H2O2 and glucose detection. Anal Chem 80(6):2250–2254

    Article  CAS  PubMed  Google Scholar 

  • Wei H, Wang E (2013) Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes. Chem Soc Rev 42(14):6060–6093

    Article  CAS  PubMed  Google Scholar 

  • Wei H, Gao L, Fan K, Liu J, He J, Qu X, Dong S, Wang E, Yan X (2021) Nanozymes: A clear definition with fuzzy edges. Nano Today 40:101269

    Article  CAS  Google Scholar 

  • Wu B, Hou L, Zhang T, Han Y, Kong C (2015) A molecularly imprinted electrochemical sensor based on a gold nanoparticle/carbon nanotube hybrid material for the sensitive detection of isoniazid. Anal Methods 7(21):9121–9129

    Article  CAS  Google Scholar 

  • Wu J, Wang X, Wang Q, Lou Z, Li S, Zhu Y, Qin L, Wei H (2019) Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes (II). Chem Soc Rev 48(4):1004–1076

    Article  CAS  PubMed  Google Scholar 

  • Xiang K, Chen G, Nie A, Wang W, Han H (2021) Silica-based nanoenzymes for rapid and ultrasensitive detection of mercury ions. Sensors Actuators B Chem 330:129304

    Article  CAS  Google Scholar 

  • Xue L, Jin N, Guo R, Wang S, Qi W, Liu Y, Li Y, Lin J (2021) Microfluidic colorimetric biosensors based on MnO2 nanozymes and convergence – divergence spiral micromixers for rapid and sensitive detection of salmonella. ACS Sens 6(8):2883–2892

    Article  CAS  PubMed  Google Scholar 

  • Yan Q, Zhi N, Yang L, Xu G, Feng Q, Zhang Q, Sun S (2020) A highly sensitive uric acid electrochemical biosensor based on a nano-cube cuprous oxide/ferrocene/uricase modified glassy carbon electrode. Sci Rep 10(1):1–10

    Article  CAS  Google Scholar 

  • Ye Y, Xiao L, He B, Zhang Q, Nie T, Yang X, Wu D, Cheng H, Li P, Wang Q (2017) Oxygen-tuned nanozyme polymerization for the preparation of hydrogels with printable and antibacterial properties. J Mater Chem B 5(7):1518–1524

    Article  CAS  PubMed  Google Scholar 

  • Yu Z, Lou R, Pan W, Li N, Tang B (2020) Nanoenzymes in disease diagnosis and therapy. Chem Commun 56:15513

    Article  CAS  Google Scholar 

  • Zeng Y, Yu D, Yu Y, Zhou T, Shi G (2012) Differential pulse voltammetric determination of methyl parathion based on multiwalled carbon nanotubes-poly(acrylamide) nanocomposite film modified electrode. J Hazard Mater 217-218:315–322

    Article  CAS  PubMed  Google Scholar 

  • Zhang X, Li G, Liu J, Su Z (2021) Bio-inspired Nanoenzyme synthesis and its application in A portable immunoassay for food allergy proteins. J Agric Food Chem 69:14751

    Article  CAS  PubMed  Google Scholar 

  • Zheng Q, Yu Y, Fan K, Ji F, Wu J, Ying Y (2016) A nano-silver enzyme electrode for organophosphorus pesticide detection. Anal Bioanal Chem 408(21):5819–5827

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors would like to acknowledge funding from the Faculty of Science: University of Johannesburg: Centre for Nanomaterials Science Research, Department of Chemical Sciences, and the National Research Foundation (Grant Number:TTK170405225933) South Africa.

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Authors and Affiliations

  1. Department of Chemical Sciences, University of Johannesburg, Johannesburg, South Africa

    Ephraim Felix Marondedze, Lukman O. Olasunkanmi & Penny Poomani Govender

  2. Department of Chemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria

    Lukman O. Olasunkanmi

  3. Water and Health Research Centre, University of Johannesburg, Johannesburg, South Africa

    Atheesha Singh

  4. Research Capacity Development, Postgraduate School: Research and Innovation, University of Johannesburg, Johannesburg, South Africa

    Penny Poomani Govender

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  1. Ephraim Felix Marondedze
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  2. Lukman O. Olasunkanmi
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  3. Atheesha Singh
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  4. Penny Poomani Govender
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Correspondence to Ephraim Felix Marondedze .

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Editors and Affiliations

  1. Department of Chemistry, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, India

    Uday Pratap Azad

  2. School of Biochemical Engineering, Indian Institute of Technology (BHU), Varanasi, Uttar Pradesh, India

    Pranjal Chandra

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Marondedze, E.F., Olasunkanmi, L.O., Singh, A., Govender, P.P. (2023). Nanoenzyme-Based Electrodes in Biomolecular Screening and Analysis. In: Azad, U.P., Chandra, P. (eds) Handbook of Nanobioelectrochemistry. Springer, Singapore. https://doi.org/10.1007/978-981-19-9437-1_22

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