Abstract
Insect cell system for conducting studies regarding its ability to produce cellular products has been prevalent in the past years. Being almost similar and just a little less versatile than mammalian cells, they can easily be manipulated using a suitable expression system. In here, the usability of the various insect cell lines has been explored. Usually for almost every other insect cell line study, the commonly used cell lines are Sf-9, Sf-21, Tn-368, and BTI-TN-5B1-4. Detailed discussion is done of the insect cell, its types, and its metabolism to understand the working of the system and identify the suitable cell line. Further on the vectors used as the expression system, the culturing of these insect cells and culturing the vector-infected cells are done. To obtain the product of interest, a cell has to undergo a lot of processes; hence, a list gist is given regarding the posttranslational modification, expression of gene in insect cell, O-glycosylation modification, and much more. Proteolysis that hinders the expression of the insect cell system can be reduced by introducing certain genes. The screening of the transformant for obtaining the products and the by-products obtained has been mentioned here. Improved expression of protein can be done by attenuating the proteolysis genes.
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Kaur, R., Kumar, A., Mandal, D. (2023). Insect Cell Factory for Production of Biomolecules. In: Singh, V., Show, P.L. (eds) Biomanufacturing for Sustainable Production of Biomolecules. Springer, Singapore. https://doi.org/10.1007/978-981-19-7911-8_14
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