Abstract
With the development of food hygiene industry, food safety has gradually become a major problem threatening human life and health. Food bacterial infection and food poisoning are the most widespread phenomena in foodborne diseases. The specific detection of foodborne pathogens is one of the effective measures to prevent foodborne diseases. At present, the commercial antibodies against foodborne pathogens are also common in the market, nevertheless, the specificity of these antibodies in the market has not been systematically evaluated. In order to better comprehend the application effect of commercial antibodies in the market, we choose Salmonella antibodies and Escherichia coli antibodies in the market as examples and evaluate the specificity of the six antibodies by enzyme-linked immunosorbent assay (ELISA), so as to find the best Salmonella antibody and Escherichia coli antibody by evaluating the experimental data.
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References
Teplitski, M., Barak, J.D., Schneider, K.R.: Human enteric pathogens in produce: un-answered ecological questions with direct implications for food safety. Curr. Opin. Biotechnol. 20, 166–171 (2009). https://doi.org/10.1016/j.copbio.2009.03.002
Sharma, A., Gautam, S., Bandyopadhyay, N.: Enzyme immunoassays:overview. Encyclopedia Food Microbiol 680–687 (2014). https://doi.org/10.1016/B978-0-12-384730-0.00099-9
Wardley, R.C., Elzein, E., Wilkinson, J.: A solid-phase enzyme linked immunosorbent assay for the detection of African swine fever virus antigen and antibody. J. Hyg. 83, 363–369 (1979). https://doi.org/10.2307/3862540
Hisanori, I., Yagami, H., et al.: Monoclonal antibodies based on hybridoma technology. Pharm. Patent Anal. (2013). https://doi.org/10.4155/ppa.13.2
Candlish, A.A.G., Smith, J.E., Stimson, W.H.: Monoclonal antibody technology for mycotoxins. Biotechnol. Adv. 401–418 (1989). https://doi.org/10.1016/0734-9750(89)90182-1
Moraes, J., Hamaguchi, B., Braggion, C., et al.: Hybridoma technology: is it still useful? (2021). https://doi.org/10.1016/j.crimmu.2021.03.002
Wong, R.M.Y., et al.: Sample sequencing of a Salmonella typhimurium LT2 lambda library: comparison to the Escherichia coli K12 genome. FEMS Microbiol. Lett. (2) (2010). https://doi.org/10.1111/j.1574-6968.1999.tb13533.x
UniProt: the universal protein knowledgebase in 2021 (2020) The UniProt Consortium. https://www.uniprot.org/uniprot/A5JP12
Miersch, S., Sidhu, S.S.: Synthetic antibodies: concepts, potential and practical considerations. Methods 57, 486–498 (2012). https://doi.org/10.1016/j.ymeth.2012.06.012
Acknowledgements
This work is supported by the Military Biosafety Research Project (grant number: A3702022004). Yan Gao and Yanan Huang contributed equally to this work.
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Gao, Y., Huang, Y., Du, S., Xia, W., Mo, F. (2023). Evaluation of Different Salmonella and Escherichia Coli Antibodies Based on ELISA. In: Long, S., Dhillon, B.S. (eds) Man-Machine-Environment System Engineering. MMESE 2022. Lecture Notes in Electrical Engineering, vol 941. Springer, Singapore. https://doi.org/10.1007/978-981-19-4786-5_24
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DOI: https://doi.org/10.1007/978-981-19-4786-5_24
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