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Synthetic Biology Tools in Cyanobacterial Biotechnology: Recent Developments and Opportunities

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Re-visiting the Rhizosphere Eco-system for Agricultural Sustainability

Part of the book series: Rhizosphere Biology ((RHBIO))

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Abstract

Cyanobacteria are ubiquitous microorganisms that play a significant role in the maintenance of the earth’s ecology. Owing to the smaller and completely sequenced genome, some strains have emerged as appropriate candidates for manipulating their genetic sequences to enhance growth and photosynthesis under distinct environmental fluctuations. Synthetic biology tools have arisen as an indispensable means for scaling up the natural circadian rhythm of prokaryotes and eukaryotes, thus improving the physiological and metabolic processes to promote their growth under adverse environmental conditions. Although the availability of synthetic biology tools for engineering multiple pathways in cyanobacteria is still limited, in the past few years significant progress has been made in developing genetic tools including promoters, sRNA, RBS, riboswitches and CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas-9 systems for engineering cyanobacteria with improved biomass production and product development. Systematic rewiring of physiological, biochemical and molecular pathways may significantly improve the growth and production of engineered cyanobacteria under stressful environments. In this chapter, recent advancement in synthetic biology tools and their application in cyanobacteria for sustainable biotechnologies is reviewed. Furthermore, it also provides valuable insights into their future developments.

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Acknowledgements

L.C. Rai thanks the Indian Council of Agricultural Research-National Bureau of Agriculturally Important Microorganisms (ICAR-NBAIM), SERB Govt of India and NASI Senior Scientist Platinum Jubilee Fellowship for financial support. Krishna Kumar Rai is thankful to NASI for Research associateship (RA), Ruchi Rai to DST-New Delhi for Women Scientist Scheme A (WOSA), and Shilpi Singh to DS Kothari, UGC, New Delhi for Post-Doctoral Fellowship (DSKPDF).

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Authors and Affiliations

  1. Molecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, Uttar Pradesh, India

    Krishna Kumar Rai, Ruchi Rai, Shilpi Singh & L. C. Rai

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  1. Krishna Kumar Rai
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  2. Ruchi Rai
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  3. Shilpi Singh
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  4. L. C. Rai
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Correspondence to L. C. Rai .

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Editors and Affiliations

  1. Plant-Microbe Interaction & Rhizosphere Biology Lab, ICAR-National Bureau of Agriculturally Important Microorganisms, Kushmaur, Uttar Pradesh, India

    Udai B. Singh

  2. Department of Mycology and Plant Pathology (BHU-KVK), Institute of Agricultural Sciences, Banaras Hindu University, RG South Campus, Mirzapur, Uttar Pradesh, India

    Jai P. Rai

  3. Department of Biological Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India

    Anil K. Sharma

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Rai, K.K., Rai, R., Singh, S., Rai, L.C. (2022). Synthetic Biology Tools in Cyanobacterial Biotechnology: Recent Developments and Opportunities. In: Singh, U.B., Rai, J.P., Sharma, A.K. (eds) Re-visiting the Rhizosphere Eco-system for Agricultural Sustainability. Rhizosphere Biology. Springer, Singapore. https://doi.org/10.1007/978-981-19-4101-6_10

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