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Experimental Mammalian Cell Culture-Based Assays

Practical Approach to Mammalian Cell and Organ Culture

Abstract

Mammalian cell culture-based assays offer multiple vital clues to understanding several distinct physiological and pathophysiological events related to cells. The key events in this regard comprise the effects of various drugs and toxins on cell survival, cellular metabolism and proliferation, cell adhesion and migration, programmed and nonprogrammed cell death (apoptosis/necrosis), generation of reactive oxygen species (ROS), reactive nitrogen species (RNS), and so on. This chapter briefly describes the fundamentals and importance of above-mentioned cellular events and protocols to track cellular events such as cell proliferation along with the pros and cons of every assay. All these assays have an implicit association with mammalian cell proliferation, survival, adhesion, migration, apoptosis, and measurement of ROS and RNS generation levels. A discussion of these issues will enhance the reader’s knowledge regarding mammalian cellular physiology as well as pathophysiology under in vitro (cell culture) conditions.

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Authors and Affiliations

  1. Department of Immunopathology, Research Block A, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India

    Srirupa Mukherjee

  2. School of Chemical Sciences, Central University of Gujarat, Gandhinagar, India

    Parth Malik

  3. Amity University, Noida, Uttar Pradesh, India

    Tapan Kumar Mukherjee

Authors
  1. Srirupa Mukherjee
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  2. Parth Malik
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  3. Tapan Kumar Mukherjee
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Editor information

Editors and Affiliations

  1. Amity University, Noida, Uttar Pradesh, India

    Tapan Kumar Mukherjee

  2. School of Nano Sciences, Central University of Gujrat, Gandhinagar, Gujarat, India

    Parth Malik

  3. Department of Immunopathology,, Post Graduate Institute of Medical Educa, Chandigarh, Chandigarh, India

    Srirupa Mukhopadhyay

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Mukherjee, S., Malik, P., Mukherjee, T.K. (2023). Experimental Mammalian Cell Culture-Based Assays. In: Mukherjee, T.K., Malik, P., Mukhopadhyay, S. (eds) Practical Approach to Mammalian Cell and Organ Culture. Springer, Singapore. https://doi.org/10.1007/978-981-19-1731-8_16-1

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Chapter history

  1. Latest

    Experimental Mammalian Cell Culture-Based Assays
    Published:
    20 January 2023

    DOI: https://doi.org/10.1007/978-981-19-1731-8_16-2

  2. Original

    Experimental Mammalian Cell Culture-Based Assays
    Published:
    10 December 2022

    DOI: https://doi.org/10.1007/978-981-19-1731-8_16-1

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