Abstract
Extremophilic fungi are the ones that can survive in extreme available habitats, which either can be natural or artificial. They can be divided mainly into thermophilic, psychrophilic, acidophilic, alkaliphilic, halophilic, and barophilic fungi. This chapter covers two major parts including (a) general isolation techniques, sample collection, preparation of isolation media, strain separation, cell maintenance, morphological, and molecular identifications and (b) maintenance of species, preparation of maintenance media, and preservation of extremophilic fungi. Apart from general operation procedures, the isolation and maintenance of extremophilic fungi needs special adjustments according to the obligate and tolerant needs of their handlings. This chapter is expected to be a consultative protocol resource for the students, academicians, and for industries working of extremophilic fungi.
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Talib, K.M. et al. (2022). Isolation, Culture, and Maintenance of Extremophilic Fungi. In: Sahay, S. (eds) Extremophilic Fungi. Springer, Singapore. https://doi.org/10.1007/978-981-16-4907-3_1
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