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Commercial Micropropagation of Some Economically Important Crops

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Abstract

Commercial tissue culture commenced in India in 1984 subsequent to the laudable classic work at University of Delhi using basic tissue culture for studies in developmental biology commenced way back in the 1950s, and its importance gradually recognized for application in crop improvement. Since then it has been a trend of ‘rise and fall, and rise again’ till date. The total tissue culture plant production in India is currently estimated to be 300 million plants per annum, of which banana shares approximately 50%. Scores of species in fruit, vegetable, floriculture, forest, plantation, medicinal, and aromatic plant categories are now produced through micropropagation. Next to banana, sugarcane and ornamentals, other crops such as pineapple, pomegranate, lemon, ivy gourd (Coccinea indica), pointed gourd (Trichosanthes dioica), and teasle or spine gourd (Momordica dioica), turmeric, and ginger are now commercially produced through micropropagation in India. There are challenges for the micropropagation industry producing banana and other plants in general, with many tissue culture units facing severe economic loss ending up in non-performance or closure. Some aspects of the problems are discussed in detail.

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References

  • Agri Business (2018) In a race against wilt. http://thehindubusinessline.com/economy/agribusiness/India. Accessed 23 Apr, 2018, Agri Business

  • Anis M, Ahmad N (2016) Plant tissue culture: a journey from research to commercialization. In: Anis M, Ahmad N (eds) Plant tissue culture: propagation, conservation and crop improvement. Springer, Singapore. https://doi.org/10.1007/978-981-10-1917-3_1

    Chapter  Google Scholar 

  • Anonymous (2012) Bio business-sun agrigenetics. A series of firsts. In: BT Chronicle (Official Magazine of Gujarat State Biotechnology Mission) Department of Science and Technology, Government of Gujarat. Volume VII(II), July 2012

    Google Scholar 

  • APAARI (2019) Banana tissue culture in India—a success story. Asia Pacific Association of Agricultural Research Institutions, Bangkok

    Google Scholar 

  • Bairy MW, Fennell CW, Van Staden J (2006) The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv ‘Zelig’). Sci Hortic 109:347–351

    Google Scholar 

  • Balachandran S, Bhatt SR, Chandel KPS (1990) In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Z. officinale Rosc.). Plant Cell Rep 8:521–529

    CAS  PubMed  Google Scholar 

  • Bhatt DP (2018) Commercial plant tissue culture: current scenario in India and challenges. In: National conference on biotechnological initiatives for crop improvement (BICI-2018). Bihar Agriculture University, Sabour, Dec 2018

    Google Scholar 

  • Bhatt DP, Bhatt PN (2007a) In vitro selection of salt tolerant banana and its commercial applications. In: Singh HP, Mustaffa MM (eds) Banana—new innovations. Westville Publishing House, New Delhi, pp 71–77

    Google Scholar 

  • Bhatt DP, Bhatt PN, Patel D, Jagdale D (2019) Commercial production of micropropagated Coccinea indica (Tondli) - a success story. J Agri Res 6:59–63

    Google Scholar 

  • Bhatt PN, Bhatt DP (2007b) In vitro selection of salt tolerant banana and its field trials. In: Proc national conference on banana: production and utilization of banana for economic livelihood and nutritional security, October 26, 2007. ICAR Banana Research Institute, Tiruchirappalli

    Google Scholar 

  • Bidabadi SS, Meon S, Wahab Z, Subramaniam S, Mahmood S (2012) In vitro selection and characterization of water stress tolerant lines among ethyl methane sulphonate (EMS) induced variants of banana (Musa spp., with AAA genome). Australian J Crop Sci 6(3):567–575

    CAS  Google Scholar 

  • Borah AR, Anbumalarmathi J, Sharmili SA (2019) In vitro propagation of Coccinea indica (L.) Voigt. From internodal segments. Indian. J Agric Res 53(2):202–207

    Google Scholar 

  • Chitra R (2019) Comparative studies on growth and yield of conventional and tissue culture plants of turmeric (Curcuma longa) var. CO2. J Hortic Sci 14(2):162–165

    Google Scholar 

  • Chopra RN, Bose JP (1925) Cephalandra indica (Telakucha) in diabetes. Indian J Med Res 13:11–16

    CAS  Google Scholar 

  • DBT (2016) An overview of national certification system for tissue culture raised plants (NCS-Plants NCS-TCP). dbtncstcp.nic.inhttps://dbtncstcp.nic.in/Portals/0/Images/NCS_TCP_Book.pdf

  • DBT (2020) Recognised TCPUs-NCS -TCP. dbtncstcp.nic.in›Recognised-TCPUs. Accessed 27 Oct 2020

  • DBT Stakeholders Meet (2016) Meet to spread awareness about plant tissue culture. www.business-standard.comPTI Stories»National»News

  • FAO (2020) FAO-medium term outlook, 2020. Prospects for global production and trade in bananas and tropical fruits, 2019–2028

    Google Scholar 

  • Ghantikumar S, Arulanandam JP, Henry Joseph L (2013) Micropropagation of Coccinia indica Wight & Arn.—a medicinal plant. Int J Appl Bio Res 18:1–4

    Google Scholar 

  • GOI Press Release (2020) 2nd advance estimates of 2019–20 of horticulture crops. Highlights of 2019-20 (2nd advance estimates) Press Information Bureau, Ministry of Agriculture & Farmers Welfare, Government of India, PIB.pib.gov.in.

  • Govil S, Gupta SC (1997) Commercialization of plant tissue culture in India. Plant Cell Tiss Org Cult 51:65–73

    Google Scholar 

  • Guha S, Maheshwari S (1964) In vitro production of embryos from anthers of Datura. Nature 204:497. https://doi.org/10.1038/204497a0

    Article  Google Scholar 

  • Gulati A (1988) Tissue culture of Coccinia grandis. Curr Sci 57:1232–1235

    Google Scholar 

  • Gulzar B, Moien AB, Malik Q, Mamgain J, Syeed R, Zafar N (2020) Transgenic plant tissue culture: agriculture and industrial applications. In: Transgenic technology based value addition in plant biotechnology. Academic Press, London, pp 25–49. https://doi.org/10.1016/B978-0-12-818632-9.00002-2

    Chapter  Google Scholar 

  • Hegazy AE, Aboshama HM, Emara HA, El Shahed AA (2012) Screening of in vitro produced banana plants for somaclonal variation. Acta Hortic 935:75–82. https://doi.org/10.17660/ActaHortic.2012.935.10

    Article  Google Scholar 

  • Hoque AM, Hossain S, Alam S, Arima S, Islam R (2007) Adventitious shoot regeneration from immature embryo explant obtained from female × female Momordica dioica. Plant Tissue Cult & Biotech 17(1):29–36

    Google Scholar 

  • Hossain MZ, Shibib BA, Rahman R (1992) Hypoglycaemic effects of Coccinia indica: inhibition of key gluconeogenic enzyme, glucose-6-phosphatase. Indian J Exp Biol 30(5):418–420

    CAS  PubMed  Google Scholar 

  • Hwang SC (2001) Recent development on Fusarium R&D of banana in Taiwan. In: Molina AB, Masdek NHN, Liew KW (eds) Banana Fusarium wilt management: towards sustainable cultivation. Montpellier, INIBAP, pp 39–49

    Google Scholar 

  • Imbumi MD (2004) Coccinia grandis (L.) voigt. In: GJH G, Denton OA (eds) Vegetables/légumes. PROTA (Plant Resources of Tropical Africa/Ressources végétales de l’Afrique tropicale), Wageningen, pp 227–229

    Google Scholar 

  • Jackson D, Williams S, Newby DM, Hall S, Higgins S (2016) Tissue cultured versus traditionally grown pineapples: growth and nutrient profile. J Biotechnol Biomater 6:237. https://doi.org/10.4172/2155-952X.1000237

    Article  Google Scholar 

  • Jeet S, Rahul VP, Bhanwaria R, Singh CP, Gochar R, Kumar A, Kumar K, Pal J, Singh B (2019) Exploring commercial cultivation of tissue cultured raised banana in Himalayan Shivalik Range. In: Singh B (ed) Plants of commercial values. New India Publishing Agency, New Delhi, pp 133–145

    Google Scholar 

  • Jha DK, Koneri R, Samaddar S (2017) Potential bio-resources of Momordica dioica Roxb.: a review. Int J Pharmaceut Sci Rev Res 45(2):203–209

    CAS  Google Scholar 

  • Joffe S (1993) The use of micropropagation by small scale farmers: case studies from potato seed schemes in Vietnam and Nepal. Technical Report. Natural Resources Institute, Chatham. http://gala.gre.ac.uk/13766

  • Josekutty RC, Shah S, Prathapsenan G (1993) Direct and indirect organogenesis in Coccinia indica. J Hortic Sci 68(1):31–35

    CAS  Google Scholar 

  • Karim MA, Ahmed SU (2010) Somatic embryogenesis and micropropagation in teasle gourd. Int J Environ Sci Develop 1(1):10–14

    Google Scholar 

  • Karim MA, Ullah MA (2011) In vitro regeneration of Teasle gourd. In: 2nd international conference on biotechnology and food science IPCBEE, IACSIT Press, Singapore

    Google Scholar 

  • Komal R (2011) One step method of plantlet regeneration in Trichosanthes dioica Roxb.: an approach towards cost effective and shorter protocol. Afr J Biotechnol 10(1):9–12

    Google Scholar 

  • Kumar A, Singh AK, Kaushik MS, Mishra SK, Raj P, Singh PK, Pandey KD (2017) Interaction of turmeric (Curcuma longa L.) with beneficial microbes: a review. Australas Biotechnol 7:357

    Google Scholar 

  • Kumar R (2017) Banana tissue culture in India; status, opportunities and challenges. Trends Biosci 10(45):9237–9241

    Google Scholar 

  • Kumar S, Singh M, Singh AK, Srivastava K, Banerjee MK (2003) In vitro propagation of pointed gourd (Trichosanthes dioica Roxb.). Cucurbit Genet Cooperat Rep 26:74–75

    Google Scholar 

  • Lal VR, Singh AK, Das B (2009) Studies on in vitro multiplication in pointed gourd (Trichosanthes dioica Roxb.) through single-node cuttings. Bios 1(4):163–166

    Google Scholar 

  • Lin LJ, Hsiao YY, Yang RY, Kuo CG (2007). Evaluation of ivy gourd and tropical violet as new vegetables for alleviating micronutrient deficiency. ISHS Acta Horticulturae 841. In: II International symposium on human health effects of fruits and vegetables, FAVHEALTH 2007

    Google Scholar 

  • Lu J, Ali A, He EQ, Yan G, Arak T, Gao S (2019) Establishment of an open, sugar-free tissue culture system for sugarcane micropropagation. Sugar Tech 22:8–14

    Google Scholar 

  • Meghalaya SFAC (2014) Tissue culture banana in Jaintia Hills. Meghalaya Small Farmer’s Agribusiness Consortium. meghalayasfac.nic.in/mgsfac/success_stories/stories28.php.14-3-2014

  • Mukherjee K, Ghosh NC, Datta T (1972) Coccinia indica as a potential hypoglycaemic agent. Indian J Exp Biol 5(10):347–349

    Google Scholar 

  • Mustaffa MM, Sathiamoorthy S (2003) Current status of banana research in India. In: Molina AB, Roa VN, Van den Bergh I, Maghuyop MA, Borromeo K (eds) Advancing banana and plantain R&D in Asia and the Pacific. Proc. 2nd BAPNET Steering Committee Meeting, Jakarta, pp 61–66

    Google Scholar 

  • Mythili JB, Thomas P (1999) Micropropagation of pointed gourd (Trichosanthes dioica Roxb.). Sci Hortic 79:87–90

    CAS  Google Scholar 

  • Nadgauda RS, Mascarenhas AF, Hendre RR, Jagannathan V (1978) Rapid clonal multiplication of turmeric (Curcuma longa L) plants by tissue culture. Indian J. Exp Biol 16:120–122

    CAS  Google Scholar 

  • Nayak S, Naik PK (2006) Factors effecting in vitro micro-rhizome formation and growth in Curcuma longa L and improved field performance of micropropagated plants. Science Asia 32:31–37

    CAS  Google Scholar 

  • NHB (2017). http://nhb.gov.in/Statistics.aspx?Type=State&menu

  • NHB (2018). http://nhb.gov.in/statistics/State_Level/area_prod20161718.pdf

  • Pant M, Mehta R (2016) The economics of plant tissue culture: an India perspective. J Pharmaceut Clin Res 9(3):1–2

    Google Scholar 

  • Patel AR, Ishnava KB (2015b) In vitro shoot multiplication from nodal explants of Coccinia grandis (L.) Voigt. And its antidiabetic and antioxidant activity. Asian J Biol Sci 8(2):57–71

    CAS  Google Scholar 

  • Patel MG, Ishnava KB (2015a) Momordica dioica Roxb. (spine gourd): multiple shoot induction from nodal cultures and its antidiabetic activity. J Med Plants Stud 3(6):82–88

    Google Scholar 

  • Prakash J (2000) Factors influencing the development of the micropropagation industry: the experience in India. Acta Hortic 530:165–172. https://doi.org/10.17660/actahortic.2000.530.18

    Article  Google Scholar 

  • Prakash J (2006) Micropropagation industry in India: biology and business. Acta Hortic 725:293–300. https://doi.org/10.17660/ActaHortic.2006.725.36

    Article  Google Scholar 

  • Prakash J (2012) Large-scale production of plants through micropropagation: problems, prospects and opportunities for India. In: Dhawan V (ed) Applications of biotechnology in forestry and horticulture. Springer, Boston, pp 265–274

    Google Scholar 

  • Prasath DK, Kandiannan R, Chitra VA, Muhammed Nissar J, Suresh K, Babu N (2017) Quality seed production in ginger and turmeric: Present status and future prospects. Shodh Chintan 9:163–170

    Google Scholar 

  • Press Information Bureau (2019) Government of India, Department of Agriculture, Cooperation and Farmers Welfare releases the 2017–18 Final and 2018–19 1st Advance Est. of Area and Production of Horticulture. Crops.pib.gov.in

  • Rabobank (2019) Micropropagation’s huge growth potential. www.rabobank.com. Accessed 25 Sep 2019

  • Ragavendran C, Natarajan D (2017) Role of plant tissue culture for improving the food security in India: a review update. In: Dhanarajan A (ed) Sustainable agriculture towards food security. Springer, Singapore, pp 231–262. https://doi.org/10.1007/978-981-10-6647-4_13

    Chapter  Google Scholar 

  • Reddy CR (2007) Plant tissue culture industry and women’s participation: ‘nimble fingers’ or docile labor? Gender Technol Develo 11(2):179–198. https://doi.org/10.1177/097185240701100203

    Article  Google Scholar 

  • Roses IA (1999) Medicinal plants of the world. In: Chemical constituents, traditional and modern medicinal uses. Humana Press, Totowa

    Google Scholar 

  • Salvi ND, George L, Eapen S (2001) Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants. Plant Cell Tiss Org Cult 66:113–119

    CAS  Google Scholar 

  • Sarker PFMS, Jahan R, Rahmatulla M (2008) In vitro regeneration of Coccinia grandis L. Voight. An indigenous medicinal plant of Bangladesh. Afr J Tradit Complement Altern Med

    Google Scholar 

  • Shah BN, Seth AK (2010) Pharmacological potential of Trichosanthes dioica – an edible plant. Int J Curr Res Rev 2(2):1–7

    CAS  Google Scholar 

  • Sharma GK (2004) Medical ethnobotany in the Shivalik range of the Himalayas. J Tennessee Acad Sci 7:12–14

    Google Scholar 

  • Shekhawat MS, Shekhawat NS, Harish KR, Phulwaria M, Gupta AK (2011) High frequency plantlet regeneration from nodal segment culture of female Momordica dioica (Roxb.). J Crop Sci Biotech 14:133–137

    Google Scholar 

  • Shukla SK (2017) National Certification System for tissue culture raised plants (NCS-TCP) as the unique quality management system for plant tissue culture sector: its inception, evolution, impact and way forward. Int J Trop Agric 35(3):415–423

    Google Scholar 

  • Shukla SK, Sharma P, Swarup R (2012) Impact of national certification system for tissue culture raised plants (NCS-TCP) on Indian plant tissue culture industry: a unique quality management system for commercial plant tissue culture. J Biotechnol Biomater 2:6. https://doi.org/10.4172/2155-952X.S1.009

    Article  Google Scholar 

  • Singh HP, Chundawat BS (2002) Improved technology of banana. Ministry of Agriculture, Government of India, pp 1–46

    Google Scholar 

  • Smith MK, Hamill SD, Langdon PW, Giles JE, Doogan VJ, Pegg KG (2006) Towards development of a Cavendish banana resistant to race 4 of fusarium wilt: gamma irradiation of micropropagated dwarf Parfitt (Musa spp., AA group, Cavendish subgroup). Australian J Experiment Agric 46:107–113

    Google Scholar 

  • Srivastava N, Gautam S, Meshram A (2016) A brief study on phytochemical compounds present in Coccinia cordifolia for their medicinal, pharmacological and industrial applications. World J Pharm Pharmaceut Sci 3(2):1995–2016

    Google Scholar 

  • Strosse H, Houwe IV, Panis B (2017) Banana cell and tissue culture – review. In: Jain SM, Swennen R (eds) Banana improvement: cellular, molecular biology and induced mutations. Science Publishers, Enfield

    Google Scholar 

  • Sundari UT, Sherif NA, Benjamin JHF, Rao MV (2011) Rapid micropropagation via axillary bud proliferation of Coccinia grandis (L.) Voigt. From nodal segments. Plant Tissue Cult Biotech 21(1):75–82

    Google Scholar 

  • Tamilselvan NT, Thirumalai T, Elumalai EK, Balaji R, David E (2011) Pharmacognosy of Coccinia grandis: a review. Asian Pac J Trop Biomed 2011:S299–S302

    Google Scholar 

  • Ulisses C, Camara TR, Willadino L, Meunier I, Gomes da Rocha PS, Albuquerque C (2000) In vitro selection of salt tolerant ‘Nanicão’ banana buds. Sci Agric 57(4):667–670

    CAS  Google Scholar 

  • Venkateswaran S, Pari L (2003) Effect of Coccinia indica leaf extract on plasma antioxidants in streptozotocin- induced experimental diabetes in rats. Phytother Res 17(6):605–608

    CAS  PubMed  Google Scholar 

  • Vézina A, Van den Bergh I (2017). http://www.promusa.org/GCTCV-218), http://meghalayasfac.nic.in/mgsfac/success_stories/stories28.php

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Acknowledgments

I take this opportunity to thank all my professional friends and colleagues- Rao TVLN, Subramani J, Jaganivasan R, Jagdale D and Mane R for providing some of the unpublished information. I am thankful to Mr. Sai Krishna for sharing data and pictures of ivy gourd under shade cultivation in Telangana; Mr. Rajni Patel, Gujarat, for sharing data on salt tolerant banana cultivated in his fields and Mr. Y. Shilla, Meghalaya for sharing data and pictures on TC Hill banana plantation.

I express my gratitude to Late Dr. Prashant Bhatt, founder Director of Sun Agrigenetics P.Ltd. for making commendable efforts in developing an industrial research centre in Gujarat, always supporting my efforts, and contributing to the commercial plant industry in India. I dedicate this chapter to him.

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Bhatt, D. (2021). Commercial Micropropagation of Some Economically Important Crops. In: Kumar Srivastava, D., Kumar Thakur, A., Kumar, P. (eds) Agricultural Biotechnology: Latest Research and Trends . Springer, Singapore. https://doi.org/10.1007/978-981-16-2339-4_1

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