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Extraction, Detection, and Characterization of Various Chemical Components of Trigonella foenum-graecum L. (Fenugreek) Known as a Valuable Seed in Agriculture

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Fenugreek

Abstract

Trigonella foenum-graecum L. (fenugreek) is cultivated in various countries and consumed for many goals including medicinal applications (anti-diabetic, lowering cholesterol level, antioxidant, anti-microbial, anti-ulcer, lactation aid, and anti-obesity), making food (like stew with rice), roasted grains coffee-substitute, and controlling insects in grain storages. Generally, the seeds and leaves of the crop are commonly applied in food and pharmaceutical industries and contain several active ingredients that is linked to the properties of this plant. The chemical composition of fenugreek crop includes moisture, ash, protein, mucilage, lipids, alkaloids (mainly trigonelline), amino acids (particularly 4-hydroxyisoleucine), saponins (mainly diosgenin), flavonoids (mainly quercetin, rutin, and vitexin), fibers (mainly soluble dietary fiber), polyphenols, coumarin, vitamins (mainly vitamin C), and minerals (mainly calcium, zinc, sulfur, and phosphorus). Fenugreek as a hydrocolloid (known as galactomannan) can be applied as a thickening, emulsifying, stabilizing, gelling, and encapsulating agents and also it has positive effects on the textural and appeal properties of food and pharmaceutical products. In this chapter, a review of the methods for extraction and identification of different substances present in fenugreek is evaluated.

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References

  • Abdelaal, E.M., Yousif, M.M., Shehata, A.A., & EL Mahdy, A.R. (1986). Chemical and functional properties of some legumes powder. Egyptian Journal of Food Science, 13(2), 201 – 205.

    Google Scholar 

  • Acharya, S., Thomas, J., & Basu, S. (2006). Fenugreek: an old-world crop for the new world. Biodiversity, 7, 27 – 30.

    Google Scholar 

  • Acharya, S.N., Thomas, J.E., & Basu, S.K. (2008). Fenugreek, an alternative crop for semiarid regions of North America. Crop Science, 48, 841 – 853.

    Google Scholar 

  • Ahmad, A., Alghamdi, S.S., Mahmood, K., & Afzal, M. (2016). Fenugreek a multipurpose crop: potentialities and improvements. Saudi Journal of Biological Sciences, 23, 300 – 310.

    Google Scholar 

  • Ahmed, M.A., Ibrahim, O.M., & Elham, A.B. (2010). Effect of bio and mineral phosphorus fertilizer on the growth, productivity and nutritional value of fenugreek (Trigonella foenum-graecum L.) in newly cultivated land. Research Journal of Agriculture and Biological Sciences, 6(3), 339 – 348.

    Google Scholar 

  • Akbari, S., Abdurahman, NH., Yunus, RM., Ruth Alara, O., & Abayomi, O. (2019). Extraction, characterization and antioxidant activity of fenugreek (Trigonella foenum-graecum) seed oil. Materials Science for Energy Technologies, 2, 349 – 355.

    Google Scholar 

  • Alara, O.R., Abdurahman, N.H., & Olalere, O.A. (2017). Ethanolic extraction of flavonoids, phenolics and antioxidants from Vernonia amygdalina leaf using two-level factorial design. Journal of King Saud University, 1 – 10.

    Google Scholar 

  • Aldakinah, A.A., AL-Shorbagy, M.Y., Abdallah, D.M., & El-Abhar, H.S. (2017). Trigonelline and vildagliptin antidiabetic effect: Improvement of insulin signaling pathway. Journal of Pharmacy and Pharmacology, 69(7), 856 – 864.

    Google Scholar 

  • Aljuhaimi, F., Simsek, S., Ozcan, M.M., Ghafoor, K., & Babiker, E. (2017). Effect of location on chemical properties, amino acid and fatty acid compositions of fenugreek (Trigonella foenum-graecum) seed and oils. Journal of Food Processing and Preservation, e13569.

    Google Scholar 

  • Al-Yahya, A.A. (2013). Reproductive, cytological and biochemical toxicity of fenugreek in male Swiss albino mice. African Journal of Pharmacy and Pharmacology, 7(29), 2072 – 2080.

    Google Scholar 

  • Amin, A., Alkaabi, A., Al-Falasi, S., & Daoud, S.A. (2005). Chemo preventive activities of Trigonella foenum-graecum (fenugreek) against breast cancer. Cell Biology International, 29, 687 – 694.

    Google Scholar 

  • AOAC. Official method of analysis. (1990). Association of official analytical chemists (13th ed.). Washington, DC.

    Google Scholar 

  • Augustin, J.M., Kuzina, V., Andersen, S.B., & Bak, S. (2011). Molecular activities, bio-synthesis and evolution of triterpenoid saponins. Phytochemistry, 72, 435 – 457.

    Google Scholar 

  • Bahmani, M., Mirhosseini, M., Mesripour, A., & Rafieian-Kopaei, M. (2016). A review on ethnobotanical and therapeutic uses of fenugreek (Trigonella foenum-graecum). Journal of Evidence-Based Complementary & Alternative Medicine, 21(1), 53 – 62.

    Google Scholar 

  • Bala Dhull, S., Singh Sandhu, K., Punia, S., Kaur, M., Chawla, P., & Malik, A. (2020). Functional, thermal and rheological behavior of fenugreek (Trigonella foenum-graecum L.) gums from different cultivars: A comparative study. International Journal of Biological Macromolecules, 159, 406 – 414.

    Google Scholar 

  • Basu, S.K., Acharya, S.N., Bandara, M.S., Friebel, D., & Thomas, J.E. (2009). Effects of genotype and environment on seed and forage yield in fenugreek (Trigonella foenum-graecum L.) grown in western Canada. Australian Journal of Crop Science, 3(6), 305 – 314.

    Google Scholar 

  • Belguith-Hadriche, O., Bouaziz, M., Jamoussi, K., Simmonds, M., El Feki, A., & Makni-Ayedi, F. (2013). Comparative study on hypocholesterolemic and antioxidant activities of various extracts of fenugreek seeds. Food Chemistry, 138, 1448 – 1453.

    Google Scholar 

  • Benzidia, B., Barbouchi, M., Hammouch, H., Belahbib, N., Zouarchi, M., Erramli, H., Ait Daoud, N., Badrane, N., & Hajjaji, N. (2019). Chemical composition and antioxidant activity of tannins extracted from green rind of Aloe vera L. Burm. F. Journal of King Saud University, 31(4), 1175 – 1181.

    Google Scholar 

  • Betty, R.I. (2008). The many healing virtues of fenugreek. Spice India, 1, 17 – 19.

    Google Scholar 

  • Brummer, Y., Cui, W., & Wang, Q. (2003). Extraction, purification and physicochemical characterization of fenugreek gum. Food Hydrocolloids, 17(3), 229 – 236.

    Google Scholar 

  • Buchineni, M., & Kondaveti, S. (2016). In-vitro anthelmintic activity of fenugreek leaves (aqueous extract) in Indian earthworms. The Pharma Innovation, 5, 70 – 75.

    Google Scholar 

  • Chang, Y.H., Cui, S.W., Roberts, K.T., Ng, P.K.W., & Wang, Q. (2011). Evaluation of extrusion-modified fenugreek gum. Food Hydrocolloids, 25(5), 1296 – 1301.

    Google Scholar 

  • Chapman, K.D. (2004). Occurrence, metabolism, and prospective functions of N-acylethanolamines in plants. Progress in Lipid Research, 43, 302 – 327.

    Google Scholar 

  • Chatterjee, S., Variyar, P.S., & Sharma, A. (2010). Bioactive lipid constituents of fenugreek. Food Chemistry, 119, 349 – 353.

    Google Scholar 

  • Chayut, N., Sobol, S., Nave, N., & Samach, A. (2014). Shielding flowers developing under stress: translating theory to field applications. Plants, 3, 304 – 323.

    Google Scholar 

  • Ciftci, O.N., Przybylski, R., Rudzinska, M., & Acharya, S. (2011). Characterization of fenugreek (Trigonella foenum-graecum) seed lipids. Journal of American Oil Chemistry Society, 88(10), 1603 – 1610.

    Google Scholar 

  • Dixit, P., Ghaskadbi, S., Mohan, H., & Devasagayam, T. (2005). Antioxidant properties of germinated fenugreek seeds. Phytotherapy Research, 19, 977 – 983.

    Google Scholar 

  • Durhan, A., Khetarpaul, N., & Bishnoi, S. (2002). Content of phytic acid and HCL-extractability of calcium, phosphorus and iron as affected by various domestic processing and cooking methods. Food Chemistry, 78, 9 – 14.

    Google Scholar 

  • El Nasri, N.A., & El Tinay, A. (2007). Functional properties of fenugreek (Trigonella foenum-graecum) protein concentrate. Food Chemistry, 103, 582 – 589.

    Google Scholar 

  • El-Aal (1986). Changes in gross chemical composition with emphasis on lipid and protein fractions during germination of fenugreek seeds. Food Chemistry, 22, 193 – 207.

    Google Scholar 

  • Evans, A.J., Hood, R.L., Oakenfull, D.G., & Sidhu, G.S. (1992). Relationship between structure and function of dietary fiber: a comparative study of the effects of three galactomannans on cholesterol metabolism in the rat. British Journal of Nutrition, 68, 217 – 229.

    Google Scholar 

  • Feyzi, S., Varidi, M., Zare, F., & Varidi, M.J. (2017). A comparison of chemical, structural and functional properties of fenugreek (Trigonella foenum-graecum) protein isolates produced using different defatting solvents. International Journal of Biological Macromolecules, 105, 27 – 35.

    Google Scholar 

  • Gadkari, P.V., Tu, S., Chiyarda, K., Reaney, M.J.T., & Ghosh, S. (2018). Rheological characterization of fenugreek gum and comparison with other galactomannans. International Journal of Biological Macromolecules, 119, 486 – 495.

    Google Scholar 

  • Gadkari, P.V., Reaney, M.J.T., & Ghosh, S. (2019). Assessment of gelation behaviour of fenugreek gum and other galactomannans by dynamic viscoelasticity, fractal analysis and temperature cycle. International Journal of Biological Macromolecules, 126, 337 – 344.

    Google Scholar 

  • Guardiola, F.A., Bahi, A., & Esteban, M.A. (2018). Effects of dietary administration of fenugreek seeds on metabolic parameters and immune status of gilthead seabream (Sparus aurata L.). Fish and Shellfish Immunology, 74, 372 – 379.

    Google Scholar 

  • Gyawali, R., & Ibrahim, S.A. (2014). Natural products as antimicrobial agents. Food Control, 46, 412 – 429.

    Google Scholar 

  • Hamden, K., Keskes, H., Belhaj, S., Mnafgui, K., & Allouche, N. (2011). Inhibitory potential of omega-3 fatty acid and fenugreek essential oil on key enzymes and carbohydrate-digestion and hypertension in diabetes rats. Lipids in Health and Disease, 10, 1 – 10.

    Google Scholar 

  • Haouala, R., Kanfir, R., Tarchoun, A., Hawala, S., & Beji, M. (2008). Allelopathic potential of Trigonella foenum-graecum L. Allelopathy Journal, 21, 307 – 316.

    Google Scholar 

  • He, Y., Ding, C., Wang, X., Wang, H., & Suo, Y. (2015). Using response surface methodology to optimize countercurrent chromatographic separation of polyphenol compounds from fenugreek (Trigonella foenum-graecum L.) seeds. Journal of Liquid Chromatography, 38(1), 29 – 35.

    Google Scholar 

  • Herrera, T., Navarro del Hierro, J., Fornari, T., Reglero, G., & Martin, D. (2019). Acid hydrolysis of saponin-rich extracts of quinoa, lentil, fenugreek and soybean to yield saponin-rich extracts and other bioactive compounds. Journal of Science of Food and Agriculture, 99, 3157 – 3167.

    Google Scholar 

  • Hooda, S., & Jood, S. (2005). Organoleptic and nutritional evaluation of wheat biscuits supplemented with untreated and treated fenugreek flour. Food Chemistry, 90, 427 – 435.

    Google Scholar 

  • Im, K.K., & Maliakel, B. (2008). Fenugreek dietary fiber a novel class of functional food ingredient. Agro Food Industry Hi-Tech, 19, 18 – 21.

    Google Scholar 

  • Jani, R., Udipi, S., & Ghugre, P. (2009). Mineral content of complementary foods. Indian Journal of Pediatrics, 76(1), 37 – 44.

    Google Scholar 

  • Jasim, B., Geethu, P.R., Mathew, J., & Radhakrishnan, E.K. (2015). Effect of endophytic Bacillus sp. from selected medicinal plants on growth promotion and diosgenin production in Trigonella foenum-graecum. Plant Cell, Tissue and Organ Culture, 122(3), 565 – 572.

    Google Scholar 

  • Jin, Y.L., Shi, Y., Zou, G., Miao, C.S., Sun, B., & Li, C. (2014). Fenugreek prevents the development of STZ-induced diabetic nephropathy in a rat model of diabetes. Evidence-Based Complementary and Alternative Medicine, 1, 1 – 10.

    Google Scholar 

  • Kasran, M., Cui, S., & Goff, H. (2013). Emulsifying properties of soy whey protein isolate – fenugreek gum conjugates in oil-in-water emulsion model system. Food Hydrocolloids, 30(2), 691 – 697.

    Google Scholar 

  • Kenny, O., Smyth, T.J., Hewage, C.M., & Brunton, N.P. (2013). Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of fenugreek (Trigonella foenum-graecum) seeds and bitter melon (Momordica charantia) fruit. Food Chemistry, 141, 4295 – 4302.

    Google Scholar 

  • Khorshidian, N., Yousefi Asli, M., Arab, M., Adeli Mirzaei, A., & Mortazavian, A.M. (2016). Fenugreek: potential applications as a functional food and nutraceutical. Nutrition and Food Sciences Research, 3(1), 5 – 16.

    Google Scholar 

  • Kiosseoglu, A.G., Doxastakis, S., & Alevisopoulos, K.S. (1999). Functional properties of Amaranth seed protein isolate. International Journal of Food Technology, 34, 253 – 260.

    Google Scholar 

  • Kiralan, M., Yorulmaz, A., Calikoglu, E., & Bayrak, A. (2017). Fatty acid and sterol composition of fenugreek seed (Trigonella foenum-graecum L.). Derim, 34, 138 – 141.

    Google Scholar 

  • Knott, E.J., Richard, A.J., Mynatt, R.L., Ribnicky, D., Stephens, J.M., & Bruce-Keller, A. (2017). Fenugreek supplementation during high-fat feeding improves specific markers of metabolic health. Scientific Reports, 7, 1 – 15.

    Google Scholar 

  • Kumar Shukla, A., Kumar, M., Singh Bishnoi, R., & Prakash Jain, C. (2017). Application of fenugreek seed gum: In novel drug delivery. Asian Journal of Biomaterial Research, 3(6), 1 – 10.

    Google Scholar 

  • Lambert, J.P., & Cormier, A. (2001). Potential interaction between warfarin and boldo-fenugreek. Pharmacotherapy, 21, 509 – 512.

    Google Scholar 

  • Laroubi, A., Touhami, M., Farouk, L., Zrara, I., Aboufatima, R., Benharref, A., & Chait, A. (2007). Prophylaxis effect of Trigonella foenum-graecum L. seeds on renal stone formation in rats. Phytotherapy Research, 21, 921 – 925.

    Google Scholar 

  • Liu, Y., Lei, F., He, L., Xu, W., & Jiang, J. (2020). Physicochemical characterization of galactomannans extracted from seeds of Gleditsia sinensis Lam and fenugreek. Comparison with commercial guar gum. International Journal of Biological Macromolecules, 158, 1047 – 1054.

    Google Scholar 

  • Madar, Z., & Shomer, I. (1990). Polysaccharide composition of a gel fraction derived from fenugreek and its effect on starch digestion and bile acid absorption in rats. Journal of Agricultural and Food Chemistry, 38(7), 1535 – 1539.

    Google Scholar 

  • Madar, Z., & Stark, A.H. (2003). New legume sources as therapeutic agents. British Journal of Nutrition, 88, 287 – 292.

    Google Scholar 

  • Mathur, P., & Chaudhary, M. (2009). Effect of domestic processing on proximate composition of fenugreek seeds. Journal of Food Science and Technology, 46, 255 – 258.

    Google Scholar 

  • Mazza, G., Di Tommaso, D., & Foti, S. (2002). Volatile constituents of sicilian fenugreek (Trigonella foenum-graecum L.) seeds. Sciences des Aliments, 22, 249 – 264.

    Google Scholar 

  • Mebaza, R., Mahmoudi, A., Fouchet, M., Dos Santos, M., Kamissoko, F., Nafti, A., Ben Cheikh, R., Rega, B., & Camel, V. (2009). Characterization of volatile compounds in Tunisian fenugreek seeds. Food Chemistry, 115, 1326 – 1336.

    Google Scholar 

  • Mehrafarin, A., Rezazadeh, SH., Naghdi Badi, H., Noormohammadi, GH., Zand, E., & Qaderi, A. (2011). A review on biology, cultivation and biotechnology of Fenugreek (Trigonella foenum-graecum L.) as a valuable medicinal plant and multipurpose. Journal of Medicinal Plants, 10(37), 6 – 24.

    Google Scholar 

  • Mohammadi, R., & Mortazavian, A.M. (2011). Technological aspects of prebiotics in probiotic fermented milks. Food Reviews International, 27, 192 – 212.

    Google Scholar 

  • Moradi Kor, Z., & Bayati Zadeh, J. (2013). Fenugreek (Trigonella foenum-graecum L.) as a valuable medicinal plant. International Journal of Advanced Biological and Biomedical Research, 1(8), 922 – 931.

    Google Scholar 

  • Naidu, M.M., Shyamala, B.N., Naik, J.P., Sulochanamma, G., & Srinivas, P. (2011). Chemical composition and antioxidant activity of the husk and endosperm of fenugreek seeds. LWT – Food Science and Technology, 44, 451 – 456.

    Google Scholar 

  • Nayak, A.K., Pal, D., & Das, S. (2013). Calcium pectinate-fenugreek seed mucilage mucoadhesive beads for controlled delivery of metformin HCL. Carbohydrate Polymers, 96(1), 349-357.

    Google Scholar 

  • Nematollahi, A., Sohrabvandi, S., Mortazavian, A.M., & Jazaeri, S. (2016). Viability of probiotic bacteria and some chemical and sensory characteristics in cornelian cherry juice during cold storage. Electronic Journal of Biotechnology, 21, 49 – 53.

    Google Scholar 

  • Nickel, J., Spanier, L.P., Botelho, F.T., Gularte, M.A., & Helbig, E. (2016). Effect of different types of processing on the total phenolic compound content, antioxidant capacity, and saponin content of Chenopodium quinoa Wild grains. Food Chemistry, 209, 139 – 143.

    Google Scholar 

  • Niknam, R., Ghanbarzadeh, B., Ayaseh, A., Rezagholi, F. (2018). The effects of Plantago major seed gum on steady and dynamic oscillatory shear rheology of sunflower oil-in-water emulsions. Journal of Texture Studies, 49(5), 536 – 547.

    Google Scholar 

  • Niknam, R., Ghanbarzadeh, B., Ayaseh, A., & Adun, P. (2019a). Comprehensive study of intrinsic viscosity, steady and oscillatory shear rheology of Barhang seed hydrocolloid in aqueous dispersions. Journal of Food Process Engineering, DOI: https://doi.org/10.1111/jfpe.13047.

  • Niknam, R., Ghanbarzadeh, B., Ayaseh, A., & Hamishehkar, H. (2019b). Plantago major seed galactomannan based biodegradable films: Effects of various plant oils on microstructure and physicochemical properties of emulsified films. Polymer Testing, 77, 105868.

    Google Scholar 

  • Niknam, R., Ghanbarzadeh, B., Ayaseh, A., & Rezagholi, F. (2019c). The hydrocolloid extracted from Plantago major seed: Effects on emulsifying and foaming properties. Journal of Dispersion Science and Technology, 41(5), 667 – 673.

    Google Scholar 

  • Niknam, R., Mousavi, M., & Kiani, H. (2020a). New studies on galactomannan extracted from Trigonella foenum-graecum (fenugreek) seed: Effect of subsequent use of ultrasound and microwave on the physicochemical and rheological properties. Food and Bioprocess Technology, 13(5), 882 – 900.

    Google Scholar 

  • Niknam, R., Ghanbarzadeh, B., Ayaseh, A., & Rezagholi, F. (2020b). Barhang (Plantago major L.) seed gum: Ultrasound-assisted extraction optimization, characterization and biological activities. Journal of Food Processing and Preservations. DOI: https://doi.org/10.1111/jfpp.14750.

  • Omezzine, F., Bouazizi, M., Simmonds, M., & Haouala, R. (2014). Variation in chemical composition and allelopathic potential of mixoploid Trigonella foenum-graecum L. with developmental stages. Food Chemistry, 148, 188 – 195.

    Google Scholar 

  • Pandey, H., & Awasthi, P. (2015). Effect of processing techniques on nutritional composition and antioxidant activity of fenugreek (Trigonella foenum-graecum) seed flour. Journal of Food Science and Technology, 52(2), 1054 – 1060.

    Google Scholar 

  • Raghuram, T.C., Sharma, R.D., & Sivakumar, B. (1994). Effect of fenugreek seeds on intravenous glucose disposition in non-insulin dependent diabetic patients. Phytotherapy Research, 8, 83 – 86.

    Google Scholar 

  • Rahmati, N.F., Koocheki, A., Varidi, M., & Kadkhodaee, R. (2017). Adsorption of speckled sugar bean protein isolate at oil-water interface: effect of ionic strength and pH. International Journal of Biological Macromolecules, 95, 1179 – 1189.

    Google Scholar 

  • Ramesh, H.P., Yamaki, K., Ono, H., & Tushida, T. (2001). Two dimensional NMR spectroscopic studies of fenugreek (Trigonella foenum-graecum) galactomannan without chemical fragmentation. Carbohydrate Polymers, 45, 69 – 77.

    Google Scholar 

  • Rashid, F., Hussain, S., & Ahmed, Z. (2018). Extraction, purification and characterization of galactomannan from fenugreek for industrial utilization. Carbohydrate Polymers, 180, 88 – 95.

    Google Scholar 

  • Ravindran, G., Carr, A., & Hardacre, A. (2011). A comparative study of the effects of three galactomannans on the functionality of extruded pea – rice blends. Food Chemistry, 124, 1620 – 1626.

    Google Scholar 

  • Riasat, M., Heidari, B., Pakniyat, H., & Jafari, A.A. (2018). Assessment of variability in secondary metabolites and expected response to genotype selection in fenugreek (Trigonella spp.). Industrial Crops and Products, 123, 221 – 231.

    Google Scholar 

  • Salarbashi, D., & Tafaghodi, M. (2018). An update on physicochemical and functional properties of newly seed gums. International Journal of Biological Macromolecules, 119, 1240 – 1247.

    Google Scholar 

  • Salarbashi, D., Bazeli, J., & Fahmideh-Rad, E. (2019). Fenugreek seed gum: Biological properties, chemical modifications, and structural analysis – A review. International Journal of Biological Macromolecules, 138, 386 – 393.

    Google Scholar 

  • Seena, S., & Sridhar, K.R. (2005). Physicochemical, functional and cooking properties of Canavalia. Food Chemistry, 32, 406 – 412.

    Google Scholar 

  • Shakuntala, S., Pura Naik, J., Jeyarani, T., Naidu, M., & Srinivas, P. (2011a). Characterization of germinated fenugreek (Trigonella foenum-graecum) seed fractions. International Journal of Food Science & Technology, 46, 2337 – 2343.

    Google Scholar 

  • Shakuntala, S., Jarpala, PN., Thangraj, J., Madineni, MN., & Pullabhatla, S. (2011b). Characterization of germinated fenugreek (Trigonella foenum-graecum) seed fractions. International Journal of Food Science, 46, 2337 – 2343.

    Google Scholar 

  • Sharara, M.S. (2017). Effect of germination and heat treatment on chemical composition and bioactive components of fenugreek seeds. World Journal of Dairy & Food Sciences, 12(1), 33 – 41.

    Google Scholar 

  • Snehlata, H.S., & Payal, D.R. (2012). Fenugreek (Trigonella foenum-graecum L.): an overview. International Journal of Current Pharmaceutical Research, 2, 169 – 187.

    Google Scholar 

  • Sowmya, P., & Rajyalakshmi, P. (1999). Hypocholesterolemic effect of germinated fenugreek seeds in human subjects. Plant Foods For Human Nutrition, 53(4), 359 – 365.

    Google Scholar 

  • Srinivasan, K. (2006). Fenugreek (Trigonella foenum-graecum): A review of health beneficial physiological effects. Food Reviews International, 22, 203 – 224.

    Google Scholar 

  • Sulieman, A.M.E., Ali, A.O., & Hemavathy, J. (2000). Lipid content and fatty acid composition of fenugreek (Trigonella foenum-graecum) seeds grown in Sudan. International Journal of Food Science and Technology, 43, 380 – 382.

    Google Scholar 

  • Svecova, E., & Neugebauerova, J. (2010). A study of 34 cultivars of basil (Ocimum L.) and their morphological, economic and biochemical characteristics, using standardized descriptors. Acta Universitatis Sapientiae, Alimentaria, 3, 118 – 135.

    Google Scholar 

  • Syeda, B., Muhammad, I., & Shahabuddin, M. (2008). Antioxidant activity from the extract of fenugreek seeds. Pakistan Journal of Analytical & Environmental Chemistry, 9, 78 – 83.

    Google Scholar 

  • Valette, G., Sauvaire, Y., Baccou, J.C., & Ribes, G. (1984). Hypocholesterolaemic effect of fenugreek seeds in dogs. Atherosclerosis, 50(1), 105 – 111.

    Google Scholar 

  • Venegas-Caleron, M., Ruiz-Mendez, M.V., Maetinez-Force, F., Garces, R., & Salas, J.J. (2017). Characterization of Xanthoceras sorbifolium Bunge seeds: Lipids, proteins and saponins content. Industrial Crops and Products, 109, 192 – 198.

    Google Scholar 

  • Verma, S., Yadav, S., & Sing, A. (2015). In vitro antibacterial activity and phytochemical analysis of Mangifera indica L. flower. Extracts against pathogenic microorganisms. Journal of Pharmacology & Clinical Toxicology, 3, 1053 – 1058.

    Google Scholar 

  • Wani, S.A., & Kumar, P. (2018). Fenugreek: a review on its nutraceutical properties and utilization in various food products. Journal of the Saudi Society of Agricultural Sciences, 17, 97 – 106.

    Google Scholar 

  • Yadav, R., & Kaushik, R. (2011). A study of phytochemical constituents and pharmacological actions of Trigonella foenum-graecum: a review. International Journal of Pharmacy and Technology, 3, 1022 – 1028.

    Google Scholar 

  • Yao, D., Zhang, B., Zhu, J., Zhang, Q., Hu, Y., Wang, S., Wang, Y., Cao, H., & Xiao, J. (2019). Advances on application of fenugreek seeds as functional foods: Pharmacology, clinical application, products, patents and market. Critical Reviews in Food Science and Nutrition, 60(14), 2342 – 2352.

    Google Scholar 

  • Yousefi, M., Wang, Q., Cui, S., & Barbut, S. (2009). Purification and partial physicochemical characteristics of protein free fenugreek gums. Food Hydrocolloids, 23(8), 2049 – 2053.

    Google Scholar 

  • Zentek, J., S. Gartner, L., Tedin, K., Manner, A., & Vahjen, W. (2013). Fenugreek seed affects intestinal microbiota and immunological variables in piglets after weaning. British Journal of Nutrition, 109(5), 859 – 866.

    Google Scholar 

  • Zhang, H., Xu, J., Wang, M., Xia, X., Dai, R., & Zhao, Y. (2020). Steroidal saponins and sapogenins from fenugreek and their inhibitory activity against α-glucosidase. Steroids, 161, 108690.

    Google Scholar 

  • Zhou, J., Zhou, S., & Zeng, S. (2013). Experimental diabetes treated with trigonelline: effect on b-cell and pancreatic oxidative parameters. Fundamental & Clinical Pharmacology, 27(3), 279 – 287.

    Google Scholar 

  • Zou, J., Chan, L., & Zhou, S. (2012). Trigonelline: a plant alkaloid with therapeutic potential for diabetes and central nervous system disease. Current Medicinal Chemistry, 19, 3523 – 3531.

    Google Scholar 

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Authors and Affiliations

  1. Bioprocessing and Biodetection Lab (BBL), Department of Food Science and Technology, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

    Rasoul Niknam, Hossein Kiani & Zeinab E. Mousavi

  2. Department of Food Science and Technology, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

    Mohammad Mousavi

Authors
  1. Rasoul Niknam
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  2. Hossein Kiani
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  3. Zeinab E. Mousavi
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  4. Mohammad Mousavi
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Corresponding author

Correspondence to Hossein Kiani .

Editor information

Editors and Affiliations

  1. Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    M. Naeem

  2. Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Tariq Aftab

  3. Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    M. Masroor A. Khan

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© 2021 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

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Niknam, R., Kiani, H., Mousavi, Z.E., Mousavi, M. (2021). Extraction, Detection, and Characterization of Various Chemical Components of Trigonella foenum-graecum L. (Fenugreek) Known as a Valuable Seed in Agriculture. In: Naeem, M., Aftab, T., Khan, M.M.A. (eds) Fenugreek. Springer, Singapore. https://doi.org/10.1007/978-981-16-1197-1_9

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