An Efficient Plant Regeneration System Via Leaf Derived Callus of Solanum melongena L.

Abstract

An efficient plant regeneration protocol developed of economically important vegetable crop, Solanum melongena L., through leaf-derived callus. Leaf explant from 20 days old aseptic seedling used for callus induction on MS (Murashige and Skoog) medium augmented with different concentration of 2,4-D and 2,4,5-T. A light green friable callus from leaf explant was obtained in 7.5 μM (2,4-D) which did not have regenerative potential. Differentiation of adventitious shoot was obtained on culturing the friable callus on MS medium augmented with various concentration of BA and Kinetin alone or in combination. The maximum number of adventitious shoots (65.5 ± 2.02) was obtained from leaf-derived callus on MS basal medium augmented with 2.0 μM BA and 0.1 μM kinetin. The potentiality of differentiation of nodular callus was influenced through callus age. For the induction of roots, the regenerated shoots cultured on MS medium supplemented with 0.5 μM Indole-3-acetic acid (IAA) or on hormone-free MS medium. The in vitro regenerated plantlets were acclimatized and shifted into the field. The in vitro regenerated plantlets were comparable to the parent plants. The developed plant regeneration protocol is a valuable tool in biotechnology for the improvement of eggplant crops in the plant breeding system.