Abstract
Plants are the major source of nutrients in the human diet. However, staple cereal crops lack certain amino acids and vitamins, and its nutritious content is not enough to provide a balanced diet. Rice is the most widely preferred food crop; thus, it is necessary that we enhance its nutritional content. This can be achieved through the process of biofortification using principles of genetic engineering. Rice is mainly known to be deficient in threonine and lysine. Hence, there has been great interest in using practical concepts of genetic engineering to increase the amino acid content of rice. Several studies are being carried out on overexpression of aminotransferases in order to obtain significant levels of essential amino acids. Efforts have also been taken to enhance the vitamin content of rice. Golden rice rich in vitamin A is one of the outcomes of such strategies. Similarly, transgenic approaches for the expression of enzymes responsible for synthesis of vitamin E have increased the levels considerably. Thus, genetic engineering and transgenic approaches can prove to be a solution to malnutrition, but further comprehensive study on metabolic pathways and its manipulation is necessary.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Avraham T, Badani H, Galili S, Amir R (2004) Enhanced levels of methionine and cysteine in transgenic alfalfa (Medicago sativa L.) plants over-expressing the Arabidopsis cystathionine γ-synthase gene. Plant Biotechnol J 3:71–79
Bashir K, Takahashi R, Nakanishi H, Nishizawa NK (2013) The road to micronutrient biofortification of rice: progress and prospects. Front Plant Sci 4:15
Beyer P, Al-Babili S, Ye X, Lucca P, Schaub P, Welsch R (2002) Golden rice: introducing the β-carotene biosynthesis pathway into rice endosperm by genetic engineering to defeat vitamin A deficiency. J Nutr 132(3):506S–510S. https://doi.org/10.1093/jn/132.3.506S
Blancquaert D, Van Daele J, Strobbe S, Kiekens F, Storozhenko S, De Steur H (2015) Improving folate (vitamin B9) stability in biofortified rice through metabolic engineering. Nat Biotechnol 33:1076–1078. https://doi.org/10.1038/nbt.3358
Bouis HE, Saltzman A (2017) Improving nutrition through biofortification: a review of evidence from Harvest Plus, 2003 through 2016. Global Food Sec 12:49–58
Bourgis F, Roje S, Nuccio ML, Fisher DB, Tarczynski MC, Li CJ, Herschbach C, Rennenberg H, Pimenta MJ, Shen TL, Gage DA, Hanson AD (1999) S-methylmethionine plays a major role in phloem sulfur transport and is synthesized by a novel type of methyltransferase. Plant Cell 11:1485–1497
Bu Y, Sun B, Zhou A, Zhang X, Takano T, Liu S (2016) Overexpression of AtOxR gene improves abiotic stresses tolerance and vitamin C content in Arabidopsis thaliana. BMC Biotechnol 16:69
Burkhardt PK, Beyer P, Wuenn J, Kloeti A, Armstrong GA, Schledz M (1997) Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis. Plant J 11:1071–1078. https://doi.org/10.1046/j.1365-313X.1997.11051071.x
Capell T, Christou P (2004) Progress in plant metabolic engineering. Curr Opin Biotechnol 15:148–154
Chen Z, Young TE, Ling J, Chang S, Gallie DR (2003) Increasing vitamin C content of plants through enhanced ascorbate recycling. Proc Natl Acad Sci U S A 100:3525–3530
Chen S, Li H, Liu G (2006) Progress of vitamin E metabolic engineering in plants. Transgenic Res 15:655–665
Cho ES, Anderson HL, Wixon RI, Hanson KC, Krause GF (1984) Long-term effects of low histidine intake on men. J Nutr 114:369–384
Collakova E, DellaPenna D (2003) The role of homogentisate phytyltransferase and other tocopherol pathway enzymes in the regulation of tocopherol synthesis during abiotic stress. Plant Physiol 133:930–940
Combs CF Jr, McClung JP (2017) The vitamins − fundamental aspects in nutrition and health, 5th edn. Academic Press, Chennai
Crowell EF, McGrath JM, Douches DS (2007) Accumulation of vitamin E in potato (Solanum tuberosum) tubers. Transgenic Res 17:205–217
Datta K, Baisakh N, Oliva N, Torrizo L, Abrigo E, Tan J (2003) Bioengineered ‘golden’ Indica rice cultivars with beta-carotene metabolism in the endosperm with hygromycin and mannose selection systems. Plant Biotechnol J 1:81–90. https://doi.org/10.1046/j.1467-7652.2003.00015.x
Dwivedi S, Mishra A, Tripathi P, Dave R, Kumar A, Srivastava S, Chakrabarty D, Trivedi PK, Adhikari B, Norton GJ (2012) Arsenic affects essential and non essential amino acids differentially in rice grains: inadequacy of amino acids in rice based diet. Environ Int 46:16–22
Edem DO (2009) Vitamin A: a review. Asian J Clin Nutr 1:65–82
FAO (2009) More people than ever are victims of hunger. http://www.fao.org/fileadmin/user_upload/newsroom/docs/Press%20release%20june-en.pdf
FAO, IFAD, WFP (2015) The state of food insecurity in the world 2015. FAO, Rome
Galili G (1995) Regulation of lysine and threonine synthesis. Plant Cell 7:899–906
Galili G, Amir R (2013) Fortifying plants with the essential amino acids lysine and methionine to improve nutritional quality. Plant Biotechnol J 11:211–222
Galili G, Hӧfgen R (2002) Metabolic engineering of amino acids and storage proteins in plants. Metab Eng 4:3–11
Gallardo F, Fu J, Jing ZP, Kirby EG, Cánovas FM (2003) Genetic modification of amino acid metabolism in woody plants. Plant Physiol Biochem 41:587–594
Garg M, Sharma N, Sharma S, Kapoor P, Kumar A, Chunduri V, Arora P (2018) Biofortified crops generated by breeding, agronomy and transgenic approaches are improving lives of millions of people around the world. Front Nut 5:12. https://doi.org/10.3389/fnut.2018.00012
Gerdes S, Lerma-Ortiz C, Frelin O, Seaver SMD, Henry CS, de Crécy-Lagard V, Hanson AD (2012) Plant B vitamin pathways and their compartmentation: a guide for the perplexed. J Exp Bot 63:5379–5395
Giuliano G (2017) Provitamin A biofortification of crop plants: a gold rush with many miners. Curr Opin Biotechnol 44:169–180
Gorelova V, Ambach L, Rébeillé F, Stove C, Van Der Straeten D (2017) Folates in plants: research advances and progress in crop biofortification. Front Chem 5:21. https://doi.org/10.3389/fchem.2017.00021
Graham RD, Welch RM, Bouis HE (2001) Addressing micronutrient malnutrition through enhancing the nutritional quality of staple foods: principles, perspectives and knowledge gaps. Adv Agron 70:77–142
Hagan ND, Upadhyaya N, Tabe LM, Higgins TJV (2003) The redistribution of protein sulfur in transgenic rice expressing a gene for a foreign, sulfur-rich protein. Plant J 34:1–11
Harish MC, Dachinamoorty P, Balamurugan S, Bala Murugan S, Sathishkumar R (2013) Overexpression of homogentisate phytyltransferase (HPT) and tocopherol cyclase (TC) enhances α-tocopherol content in transgenic tobacco. Biol Plant 57:395–400
Hirschberg J (1999) Production of high-value compounds: carotenoids and vitamin E. Curr Opin Biotechnol 10:186–191
Huang Z, Liu Y, Qi G, Brand D, Zheng SG (2018) Role of vitamin A in the immune system. J Clin Med 7(9):258. https://doi.org/10.3390/jcm7090258
Jiang J, Chen Z, Ban L, Wu Y, Huang J, Chu J, Fang S, Wang Z, Gao H, Wang X (2017a) P-hydroxyphenylpyruvate dioxygenase from Medicago sativa is involved in vitamin E biosynthesis and abscisic acid mediated seed germation. Sci Rep 7:40625
Jiang L, Wang W, Lian T, Zhang C (2017b) Manipulation of metabolic pathways to develop vitamin-enriched crops for human health. Front Plant Sci 8:937. https://doi.org/10.3389/fpls.2017.00937
Juan PA, Salvatore D (2006) Free amino acids in the nervous system of the amphioxus Branchiostoma lanceolatum. A comparative study. Int J Biol Sci 2:87–92
Katsube T, Kurisaka N, Ogawa M, Maruyama N, Ohtsuka R, Utsumi S (1999a) Accumulation of soybean glycinin and its assembly with the glutelins in rice. Plant Physiol 120:1063–1073. https://doi.org/10.1104/pp.120.4.1063
Katsube T, Kurisaka N, Ogawa M, Maruyama N, Ohtsuka R, Utsumi S, Takaiwa F (1999b) Accumulation of soybean glycinine and its assembly with the glutelins in rice. Plant Physiol 120:1063–1073
Kawagishi-Kobayashi M, Yabe N, Tsuchiya M, Harada S, Kobayashi T, Komeda Y, Kyo W (2005) Rice OASA1D, a mutant anthranilate synthase α subunit gene, is an effective selectable marker for transformation of Arabidopsis thaliana. Plant Biotechnol 22(4):271–276
Kawakatsu T, Takaiwa F (2018) Rice proteins and essential amino acids. In: Bao J (ed) Rice: chemistry and technology, 4th edn. Elsevier, Amsterdam
Kawakatsu T, Yamamoto MP, Hirose S, Yano M, Takaiwa F (2008) Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm. J Exp Bot 59:4233–4245
Le DT, Chu HD, Le NG (2016) Improving nutritional quality of plant proteins through genetic engineering. Curr Genomics 17:220–229
Lee SI, Kim HU, Lee YH, Suh SC, Lim YP, Lee HY (2001a) Constitutive and seed-specific expression of a maize lysine-feedback-insensitive dihydrodipicolinate synthase gene leads to increased free lysine levels in rice seeds. Mol Breed 8:75–84. https://doi.org/10.1023/A:1011977219926
Lee SI, Kim HU, Lee Y-H, Suh S-C, Lim YP, Lee H-Y, Kim H-I (2001b) Constitutive and seed-specific expression of a maize lysine-feedback-insensitive dihydrodipicolinate synthase gene leads to increased free lysine levels in rice seeds. Mol Breed 8:75–84
Lee TTT, Wang MMC, Hou RCW, Chen L-J, Su R-C, Wang C-S, Tzen JTC (2003) Enhanced methionine and cysteine levels in transgenic rice seeds by the accumulation of Sesame 2S Albumin. Biosci Biotechnol Biochem 67:1699–1705
Li L, van Eck J (2007) Metabolic engineering of carotenoid accumulation by creating a metabolic sink. Transgenic Res 16:581–585
Lisko KA, Torres R, Harris RS, Belisle M, Vaughan MM, Jullian B, Chevone BI, Mendes P, Nessler CL, Lorence A (2013) Elevating vitamin C content via overexpression of myo-inositol oxygenase and L-gulono-1,4-lactone oxidase in Arabidopsis leads to enhanced biomass and tolerance to abiotic stress. In Vitro Cell Dev Biol-Plant 49:643–655
Liu B, Wang L, Yang J, Zhang W, Fan Y (2008) Isolation and characterization of 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase gene promoter from Arabidopsis thaliana. Chin J Biotechnol 24:33–39
Liu X, Zhang C, Wang X, Liu Q, Yuan D, Pan G, Sun SSM, Tu J (2016) Development of high-lysine rice via endosperm-specific expression of a foreign lysine rich protein gene. BMC Plant Biol 147(16):1–13
Long X, Liu Q, Chan M, Wang Q, Sun SSM (2013) Metabolic engineering and profiling of rice with increased lysine. Plant Biotechnol J 11(4):490–501. https://doi.org/10.1111/pbi.12037
Mauro G, Pietro C (1997) Correlation between amino acid induced changes in energy expenditure and protein metabolism in humans. Nutrition 13:309–312
Miflin BJ, Habash DZ (2002) The role of glutamine synthase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops. J Exp Bot 53:979–987
Mohan M, Antony T, Malik S, Mathur M (1988) Rice powder oral rehydration solution as an alternative to glucose electrolyte solution. Indian J Med Res 87:234–239
Nguyen HC, Hoefgen R, Hesse H (2012) Improving the nutritive value of rice seeds: elevation of cysteine and methionine contents in rice plants by ectopic expression of a bacterial serine acetyltransferase. J Exp Bot 63:5991–6001
Obara M, Kajiura M, Fukuta Y, Yano M, Hayashi M, Yamaya T, Sato T (2001) Mapping of QTLs associated with cystolic glutamine synthetase and NADH-glutamate synthase in rice (Oryza sativa L.). J Exp Bot 52:567–575
Paine JA, Shipton CA, Chaggar S, Howells RM, Kennedy MJ, Vernon G (2005) Improving the nutritional value of golden rice through increased pro-vitamin A content. Nat Biotechnol 23:482–487. https://doi.org/10.1038/nbt1082
Roje S (2007) Vitamin B biosynthesis in plants. Phytochemistry 68(14):1904–1921
Ross AC, Gardner EM (1994) The function of vitamin A in cellular growth and differentiation, and its roles during pregnancy and lactation. Adv Exp Med Biol 352:187–200
Sautter C, Poletti S, Zhang P, Gruissem W (2006) Biofortification of essential nutritional compounds and trance elements in rice and cassava. Proc Nutr Soc 65:153–159
Shimaoka T, Yokota A, Miyake C (2000) Purification and characterization of chloroplast dehydroascorbate reductase from spinach leaves. Plant Cell Physiol 41:1110–1118
Sindhu AS, Zheng Z, Murai N (1997) The pea seed storage protein legumin was synthesized, processed, and accumulated stably in transgenic rice endosperm. Plant Sci 130:189–196. https://doi.org/10.1016/S0168-9452(97)00219-7
Smirnoff N (2011) Vitamin C: the metabolism and functions of ascorbic acid and plants. Adv Bot Res 59:109–177
Srivastava RK (2018) Application of agricultural biotechnology for high nutritious food products. Ann Agric Crop Sci 3(2):1–6
Storozhenko S, De Brouwer V, Volckaert M, Navarrete O, Blancquaert D, Zhang GF (2007) Folate fortification of rice by metabolic engineering. Nat Biotechnol 25(11):1277–1279. https://doi.org/10.1038/nbt1351
Sun SSM, Liu Q (2004) Transgenic approaches to improve the nutritional quality. In Vitro Cell Dev Biol–Plant 40:155–162
Tang G, Qin J, Dolnikowski GG, Russell RM, Grusak GA (2009) Golden rice is an effective source of vitamin A. Am J Clin Nutr 89:1776–1783
Tozawa Y, Hasegawa H, Terakawa T, Wakasa K (2001) Characterization of rice anthranilate synthase α-subunit genes OASA1 and OASA2. Tryptophan accumulation in transgenic rice expressing a feed-insensitive mutant of OASA1. Plant Physiol 126:1493–1506
Tripathy SK, Dash M, Behera SK, Ithape DM, Maharana M (2017) Nutrient rich quality rice – a journey to healthy life. Adv Plant Agric Res 7(5):364–367
Ufaz S, Galili G (2008) Improving the content of essential amino acids in crop plants: goals and opportunities. Plant Physiol 147:954–961
Urano J, Nakagawa T, Maki Y, Masumura T, Tanaka K, Murata N, Ushimaru T (2000) Molecular cloning and characterization of a rice dehydroascorbate reductase. FEBS Lett 466:107–111
Wakasa K, Hasegawa H, Nemoto H, Matsuda F, Miyazawa H, Tozawa Y (2006) High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile. J Exp Bot 57:3069–3078. https://doi.org/10.1093/jxb/erl068
Wang W, Galili G (2016) Transgenic high-lysine rice – a realistic solution to malnutrition? J Exp Bot 67:4009–4011
Wang L, Zhong M, Li X, Yuan D, Xu Y, Liu H, He Y, Luo L, Zhang Q (2008) The QTL controlling amino acid content in grains of rice (Oryza sativa L.) are co-localized with the regions involved in the amino acid metabolism pathway. Mol Breed 21:127–137
Wang Z, Xiao Y, Chen W, Tang K, Zhang L (2010) Increased vitamin C content accompanied by an enhanced recycling pathway confers oxidative stress tolerance in Arabidopsis. J Integr Plant Biol 52:400–409
Wu XR, Chen ZH, Folk WR (2003) Enrichment of cereal protein lysine content by altered tRNA(lys) coding during protein synthesis. Plant Biotechnol J 1:187–194
Xu JH, Messing J (2009) Amplification of prolamin storage protein genes in different subfamilies of the Poaceae. Theor Appl Genet 119:1397–1412
Yabuta Y, Tanaka H, Yoshimura S, Suzuki A, Tamoi M, Maruta T, Shigeoka S (2013) Improvement of vitamin E quality and quantity in tobacco and lettuce by chloroplast genetic engineering. Transgenic Res 22:391–402
Yang W, Cahoon RE, Hunter SC, Zhang C, Han J, Borgschulte T, Cahoon EB (2011) Vitamin E biosynthesis: functional characterization of the monocot homogentisate geranylgeranyl transferase. The Plant J 65:206–217
Yang Q-Q, Zhang C-Q, Chan M-L, Zhao D-S, Chen J-Z, Wang Q, Li Q-F, Yu H-X, Gu M-H, Sun SS-M, Liu Q-Q (2016) Biofortification of rice with the essential amino acid lysine: molecular characterization, nutritional evaluation, and field performance. J Exp Bot 67:4285–4296
Ye X, Al-Babili S, Kloti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering the provitamin A (beta-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 287:303–305
Zhang C, Cahoon RE, Hunter SC, Chen M, Han J, Cahoon EB (2012) Genetic and biochemical basis for alternative routes of tocotrienol biosynthesis for enhanced vitamin E antioxidant production. Plant J 73:628–639
Zhang C, Wohlhueter R, Zhang H (2016) Genetically modified foods: a critical review of their promise and problems. Food Sci Human Wellness 5:116–123
Zheng A, Sumi K, Tanaka K, Murai N (1995) The bean seed storage protein β-phaseolin is synthesized, processed and accumulated in the vacuolar type-II protein bodies of transgenic rice endosperm. Plant Physiol 109:777–786. https://doi.org/10.1104/pp109.3.777
Zhong M, Wang LQ, Yuan DJ, Luo LJ, Xu CG, He YQ (2011) Identification of QTL affecting protein and amino acid contents in rice. Rice Sci 18:187–195
Zhou Y, Cai H, Xiao J, Li X, Zhang Q, Lian X (2009) Over-expression of aspirate aminotransferase gene in rice resulted in altered nitrogen metabolism and increased amino acid content in seeds. Theor Appl Genet 118:1381–1390
Zou L, Li H, Ouyang B, Zhang J, Ye Z (2006) Cloning and mapping of genes involved in tomato ascorbic acid biosynthesis and metabolism. Plant Sci 170:120–127
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Thangadurai, D., Soundar Raju, C., Sangeetha, J., Hospet, R., Pandhari, R. (2020). Rice Genetic Engineering for Increased Amino Acid and Vitamin Contents. In: Roychoudhury, A. (eds) Rice Research for Quality Improvement: Genomics and Genetic Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-15-5337-0_29
Download citation
DOI: https://doi.org/10.1007/978-981-15-5337-0_29
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-15-5336-3
Online ISBN: 978-981-15-5337-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)