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Abstract

This chapter focuses on the recent developments in the electrochemical biosensor. On the onset historical aspects of biosensors have been discussed followed by the current state of the art, which includes the fabrication, immobilization of the receptors and the linking chemistry, sensitivity, specificity, and target detection species. The importance of the electrode platform is outlined and classified into porous and nonporous. The sensor reported on various platforms has been reviewed.

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References

  • Argoubi W, Sánchez A, Parrado C, Raouafi N, Villalonga R (2018) Label-free electrochemical aptasensing platform based on mesoporous silica thin film for the detection of prostate specific antigen. Sens Actuators B Chem 255:309–315

    CAS  Google Scholar 

  • Bhalla V, Carrara S, Sharma P, Nangia Y, Suri CR (2012) Gold nanoparticles mediated label-free capacitance detection of cardiac troponin I. Sens Actuators B Chem 161:761–768

    CAS  Google Scholar 

  • Biosensors Market Report. 2019. https://www.marketsandmarkets.com/PressReleases/biosensors.asp. Accessed 10 May 2019

  • Chan KY, Ye WW, Zhang Y, Xiao LD, Leung PHM, Li Y, Yang M (2013) Ultrasensitive detection of E. coli O157: H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor. Biosens Bioelectron 41:532–537

    CAS  PubMed  Google Scholar 

  • Cheng MS, Ho JS, Tan CH, Wong JPS, Ng LC, Toh CS (2012) Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus. Anal Chim Acta 725:74–80

    CAS  PubMed  Google Scholar 

  • Chitravathi S, Munichandraiah N (2016) Voltammetric determination of paracetamol, tramadol and caffeine using poly (Nile blue) modified glassy carbon electrode. J Electroanal Chem 764:93–103

    CAS  Google Scholar 

  • Clark LC Jr, Lyons C (1962) Electrode systems for continuous monitoring in cardiovascular surgery. Ann NY Acad Sci 102:29–45

    CAS  PubMed  Google Scholar 

  • Das P, Das M, Chinnadayyala SR, Singha IM, Goswami P (2016) Recent advances on developing 3rd generation enzyme electrode for biosensor applications. Biosens Bioelectron 79:386–397

    CAS  PubMed  Google Scholar 

  • De Ávila BEF, Escamilla-Gómez V, Campuzano S, Pedrero M, Salvador JP, Marco MP, Pingarrón JM (2013) Ultrasensitive amperometric magnetoimmunosensor for human C-reactive protein quantification in serum. Sens Actuators B Chem 188:212–220

    Google Scholar 

  • De la Escosura-Muñiz A, Merkoçi A (2011) A nanochannel/nanoparticle-based filtering and sensing platform for direct detection of a cancer biomarker in blood. Small 7:675–682

    PubMed  Google Scholar 

  • De La Escosura-Muñiz A, Espinoza-Castañeda M, Hasegawa M, Philippe L, Merkoçi A (2015) Nanoparticles-based nanochannels assembled on a plastic flexible substrate for label-free immunosensing. Nano Res 8:1180–1188

    Google Scholar 

  • Deng J, Toh CS (2013) Impedimetric DNA biosensor based on a nanoporous alumina membrane for the detection of the specific oligonucleotide sequence of dengue virus. Sensors 13:7774–7785

    CAS  PubMed  Google Scholar 

  • Ensafi AA, Ahmadi N, Rezaei B (2017) Nickel nanoparticles supported on porous silicon flour, application as a non-enzymatic electrochemical glucose sensor. Sens Actuators B Chem 239:807–815

    CAS  Google Scholar 

  • Ganjali MR, Nejad FG, Beitollahi H, Jahani S, Rezapour M, Larijani B (2017) Highly sensitive voltammetric sensor for determination of ascorbic acid using graphite screen printed electrode modified with ZnO/Al2O3 nanocomposite. Int J Electrochem Sci 12:3231–3240

    CAS  Google Scholar 

  • Gao HL, Wang M, Wu ZQ, Wang C, Wang K, Xia XH (2015) Morpholino-functionalized nanochannel array for label-free single nucleotide polymorphisms detection. Anal Chem 87:3936–3941

    CAS  PubMed  Google Scholar 

  • Ge S, Yu F, Ge L, Yan M, Yu J, Chen D (2012) Disposable electrochemical immunosensor for simultaneous assay of a panel of breast cancer tumor markers. Analyst 137:4727–4733

    CAS  PubMed  Google Scholar 

  • Guo T, Gao J, Qin X, Zhang X, Xue H (2018) A novel glucose biosensor based on hierarchically porous block copolymer film. Polymers 10:723

    PubMed Central  Google Scholar 

  • Hasan A, Pandey LM (2016) Kinetic studies of attachment and re-orientation of octyltriethoxysilane for formation of self-assembled monolayer on a silica substrate. Mat Sci Eng C 68:423–429

    CAS  Google Scholar 

  • Hasan A, Saxena V, Pandey LM (2018) Surface functionalization of Ti6Al4V via self-assembled monolayers for improved protein adsorption and fibroblast adhesion. Langmuir 34:3494–3506

    CAS  PubMed  Google Scholar 

  • Hashemzadeh N, Hasanzadeh M, Shadjou N, Eivazi-Ziaei J, Khoubnasabjafari M, Jouyban A (2016) Graphene quantum dot modified glassy carbon electrode for the determination of doxorubicin hydrochloride in human plasma. J Pharm Anal 6:235–241

    PubMed  PubMed Central  Google Scholar 

  • Hernandez-Vargas G, Sosa-Hernández J, Saldarriaga-Hernandez S, Villalba-Rodríguez A, Parra-Saldivar R, Iqbal H (2018) Electrochemical biosensors: A solution to pollution detection with reference to environmental contaminants. Biosensors 8:29

    PubMed Central  Google Scholar 

  • Jia F, Duan N, Wu S, Dai R, Wang Z, Li X (2016) Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubes. Microchim Acta 183:337–344

    CAS  Google Scholar 

  • Joung CK, Kim HN, Lim MC, Jeon TJ, Kim HY, Kim YR (2013) A nanoporous membrane-based impedimetric immunosensor for label-free detection of pathogenic bacteria in whole milk. Biosens Bioelectron 44:210–215

    CAS  PubMed  Google Scholar 

  • Kadefors R, Reswick JB, Martin RL (1970) A percutaneous electrode for long-term monitoring of bio-electrical signals in humans. Med & Biol Eng 8:129–135

    CAS  Google Scholar 

  • Karikalan N, Karthik R, Chen SM, Chen HA (2017) A voltammetric determination of caffeic acid in red wines based on the nitrogen doped carbon modified glassy carbon electrode. Sci Rep 7:45924

    CAS  PubMed  PubMed Central  Google Scholar 

  • Krismastuti FSH, Pace S, Voelcker NH (2014) Porous silicon resonant microcavity biosensor for matrix metalloproteinase detection. Adv Funct Mater 24:3639–3650

    CAS  Google Scholar 

  • Krismastuti FSH, Bayat H, Voelcker NH, Schönherr H (2015) Real time monitoring of layer-by-layer polyelectrolyte deposition and bacterial enzyme detection in nanoporous anodized aluminum oxide. Anal Chem 87:3856–3863

    CAS  PubMed  Google Scholar 

  • Lai G, Wang L, Wu J, Ju H, Yan F (2012) Electrochemical stripping analysis of nanogold label-induced silver deposition for ultrasensitive multiplexed detection of tumor markers. Anal Chim Acta 721:1–6

    CAS  PubMed  Google Scholar 

  • Lin J, He C, Zhang S (2009) Immunoassay channels for α-fetoprotein based on encapsulation of biorecognition molecules into SBA-15 mesopores. Anal Chim Acta 643:90–94

    CAS  PubMed  Google Scholar 

  • Lin J, Wei Z, Mao C (2011) A Label-free immunosensor based on modified mesoporous silica for simultaneous determination of tumor markers. Biosens Bioelectron 29:40–45

    CAS  PubMed  Google Scholar 

  • Lin J, Wei Z, Chu P (2012) A label-free immunosensor by controlled fabrication of monoclonal antibodies and gold nanoparticles inside the mesopores. Anal Biochem 421:97–102

    CAS  PubMed  Google Scholar 

  • Lin J, Wei Z, Zhang H, Shao M (2013) Sensitive Immunosensor for the label-free determination of tumor marker based on carbon nanotubes/mesoporous silica and graphene modified electrode. Biosens Bioelectron 41:342–347

    CAS  PubMed  Google Scholar 

  • Liu S, Ma Y, Cui M, Luo X (2018) Enhanced electrochemical biosensing of alpha-fetoprotein based on three-dimensional macroporous conducting polymer polyaniline. Sens Actuators B Chem 255:2568–2574

    CAS  Google Scholar 

  • Maniya NH (2018) Recent advances in porous silicon based optical biosensors. Rev Adv Mater Sci 53:49–73

    CAS  Google Scholar 

  • Maniya NH, Patel SR, Murthy ZVP (2015) Development and in vitro evaluation of acyclovir delivery system using nanostructured porous silicon carriers. Chem Eng Res Des 104:551–557

    CAS  Google Scholar 

  • Maniya NH, Patel SR, Murthy ZVP (2016) Drug delivery with porous silicon films, microparticles, and nanoparticles. Rev Adv Mater Sci 44:257–272

    CAS  Google Scholar 

  • Martínez-García G, Pérez-Julián E, Agüí L, Cabré N, Joven J, Yáñez-Sedeño P, Pingarrón JM (2017) An Electrochemical enzyme biosensor for 3-hydroxybutyrate detection using screen-printed electrodes modified by reduced graphene oxide and thionine. Biosensors 7:50

    PubMed Central  Google Scholar 

  • Martín-Yerga D, Costa-García A (2015) Towards a blocking-free electrochemical immunosensing strategy for anti-transglutaminase antibodies using screen printed electrodes. Bioelectrochemistry 105:88–94

    PubMed  Google Scholar 

  • Martín-Yerga D, González-García MB, Costa-García A (2014) Electrochemical immunosensor for anti-tissue transglutaminase antibodies based on the situ detection of quantum dots. Talanta 130:598–602

    PubMed  Google Scholar 

  • McNaught AD, Wilkinson A (1997) IUPAC. Compendium of chemical terminology, Gold Book. Blackwell Scientific Publications, Oxford

    Google Scholar 

  • Medintz IL, Uyeda HT, Goldman ER, Mattoussi H (2005) Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater 4:435–446

    CAS  PubMed  Google Scholar 

  • Mondal A, Paul A, Srivastava DN, Panda AB (2018) NiO hollow microspheres as efficient bifunctional electrocatalysts for overall water-splitting. Int J Hydrogen Energy 43:21665–21674

    CAS  Google Scholar 

  • Movlaee K, Norouzi P, Beitollahi H, Rezapour M, Larijani B (2017) Highly selective differential pulse voltammetric determination of uric acid using modified glassy carbon electrode. Int J Electrochem Sci 12:3241–3251

    CAS  Google Scholar 

  • Neves MMPS, González-García MB, Santos-Silva A, Costa-García A (2012) Voltammetric immunosensor for the diagnosis of celiac disease on the quantification of anti-gliadin antibodies. Sens Actuators B Chem 163:253–259

    CAS  Google Scholar 

  • Nguyen BTT, Koh G, Lim HS, Chua AJS, Ng MML, Toh CS (2009) Membrane-based electrochemical nanobiosensor for the detection of virus. Anal Chem 81:7226–7234

    CAS  PubMed  Google Scholar 

  • Pandey LM, Pattanayak SK, Delabouglise D (2013) Properties of adsorbed bovine serum albumin and fibrinogen on self-assembled monolayers. J Phys Chem C 117:6151–6160

    CAS  Google Scholar 

  • Pandiaraj M, Sethy NK, Bhargava K, Kameswararao V, Karunakaran C (2014) Designing label-free electrochemical immunosensors for cytochrome c using nanocomposites functionalized screen-printed electrodes. Biosens Bioelectron 54:115–121

    CAS  PubMed  Google Scholar 

  • Paul A, Chiriaco MS, Primiceri E, Srivastava DN, Maruccio G (2019) Picomolar detection of retinol binding protein 4 for early management of type II diabetes. Biosens Bioelectron 128:122–128

    CAS  PubMed  Google Scholar 

  • Peh AEK, Li SFY (2013) Dengue virus detection using impedance measured across nanoporous alumina membrane. Biosens Bioelectron 42:391–396

    CAS  PubMed  Google Scholar 

  • Peng Z, Jiang Z, Huang X, Li Y (2016) A novel electrochemical sensor of tryptophan based on silver nanoparticles/metal–organic framework composite modified glassy carbon electrode. RSC Adv 6:13742–13748

    CAS  Google Scholar 

  • Perween M, Srivastava DN (2017) A cost-effective, unmodified platform for the detection of heavy metals via anodic stripping voltammetry at nanomolar level. Chem Select 2:4428–4432

    CAS  Google Scholar 

  • Perween M, Srivastava DN (2018) Designing ionic-liquid based practical reference electrode. Ind J Chem Tech 25:74–80

    CAS  Google Scholar 

  • Perween M, Parmar DB, Bhadu GR, Srivastava DN (2014) Polymer-graphite composite: a versatile use and throw plastic chip electrode. Analyst 139:5919–5926

    CAS  PubMed  Google Scholar 

  • Piro B, Reisberg S (2017) Recent advances in electrochemical immunosensors. Sensors 17:794

    Google Scholar 

  • Rai V, Deng J, Toh CS (2012) Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp. Talanta 98:112–117

    CAS  PubMed  Google Scholar 

  • Rajeev G, Simon BP, Marsal LF, Voelcker NH (2018) Advances in nanoporous anodic alumina-based biosensors to detect biomarkers of clinical significance: a review. Adv Healthcare Mater 7:1700904

    Google Scholar 

  • Ravalli A, Pilon dos Santos G, Ferroni M, Faglia G, Yamanaka H, Marrazza G (2013) New label free CA125 detection based on gold nanostructured screen-printed electrode. Sens Actuators B Chem 179:194–200

    CAS  Google Scholar 

  • Renedo OD, Alonso-Lomillo MA, Martínez MA (2007) Recent developments in the field of screen-printed electrodes and their related applications. Talanta 73:202–219

    CAS  PubMed  Google Scholar 

  • Reta N, Michelmore A, Saint C, Prieto-Simon B, Voelcker NH (2016) Porous silicon membrane-modified electrodes for label-free voltammetric detection of MS2 bacteriophage. Biosens Bioelectron 80:47–53

    CAS  PubMed  Google Scholar 

  • Reta N, Saint CP, Michelmore A, Prieto-Simon B, Voelcker NH (2018) Nanostructured electrochemical biosensors for label-free detection of water- and food-borne pathogens. ACS Appl Mater Interfaces 10:6055–6072

    CAS  PubMed  Google Scholar 

  • Ronkainen NJ, Halsall HB, Heineman WR (2010) Electrochemical biosensors. Chem Soc Rev 39:1747–1763

    CAS  PubMed  Google Scholar 

  • Roushani M, Ghanbari K (2019) An electrochemical aptasensor for streptomycin based on covalent attachment of the aptamer onto a mesoporous silica thin film-coated gold electrode. Microchim Acta 186:115

    Google Scholar 

  • Silva BVM, Cavalcanti IT, Silva MMS, Dutra RF (2013) A carbon nanotube screen-printed electrode for label-free detection of the human cardiac troponin T. Talanta 117:431–437

    CAS  PubMed  Google Scholar 

  • Szili EJ, Jane A, Low SP, Sweetman M, Macardle P, Kumar S, Smart RSC, Voelcker NH (2011) Interferometric porous silicon transducers using an enzymatically amplified optical signal. Sens Actuators B Chem 160:341–348

    CAS  Google Scholar 

  • Talarico D, Arduini F, Amine A, Cacciotti I, Moscone D, Palleschi G (2016) Screen-printed electrode modified with carbon black and chitosan: a novel platform for acetylcholinesterase biosensor development. Anal Bioanal Chem 408:7299–7309

    CAS  PubMed  Google Scholar 

  • Taleat Z, Khoshroo A, Mazloum-Ardakani M (2014) Screen-printed electrodes for biosensing: a review (2008-2013). Microchim Acta 181:865–891

    CAS  Google Scholar 

  • Tallapragada SD, Layek K, Mukherjee R, Mistry KK, Ghosh M (2017) Development of screen-printed electrode based immunosensor for the detection of HER2 antigen in human serum samples. Bioelectrochemistry 118:25–30

    CAS  PubMed  Google Scholar 

  • Thevenot DR, Toth K, Durst RA, Wilson GS (2001) Electrochemical biosensors: recommended definitions and classification. Biosens Bioelectron 16:121–131

    CAS  PubMed  Google Scholar 

  • Tian F, Lyu J, Shi J, Tan F, Yang M (2016) A polymeric microfluidic device integrated with nanoporous alumina membranes for simultaneous detection of multiple foodborne pathogens. Sens Actuators B Chem 225:312–318

    CAS  Google Scholar 

  • Tucking KS, Vasani RB, Cavallaro AA, Voelcker NH, Schonherr H, Prieto-Simon B (2018) Hyaluronic acid-modified porous silicon films for the electrochemical sensing of bacterial hyaluronidase. Macromol Rapid Commun 39:1800178

    Google Scholar 

  • Vlassiouk I, Takmakov P, Smirnov S (2005) Sensing DNA hybridization via ionic conductance through a nanoporous electrode. Langmuir 21:4776–4778

    CAS  PubMed  Google Scholar 

  • Vomero M, Oliveira A, Ashouri D, Eickenscheidt M, Stieglitz T (2018) Graphitic carbon electrodes on flexible substrate for neural applications entirely fabricated using infrared nanosecond laser technology. Sci Rep 8:14749

    PubMed  PubMed Central  Google Scholar 

  • Wang X, Xi M, Guo M, Sheng F, Xiao G, Wu S, Uchiyama S, Matsuura H (2016) An electrochemically aminated glassy carbon electrode for simultaneous determination of hydroquinone and catechol. Analyst 141:1077–1082

    CAS  PubMed  Google Scholar 

  • Windmiller JR, Wang J (2013) Wearable electrochemical sensors and biosensors: a review. Electroanalysis 25:29–46

    CAS  Google Scholar 

  • Wu Y, Xue P, Kang Y, Hui KM (2013) Paper based microfluidic electrochemical immunodevice integrated with nanobioprobes onto graphene film for ultrasensitive multiplexed detection of cancer biomarkers. Anal Chem 85:8661–8668

    CAS  PubMed  Google Scholar 

  • Wu S, Ye WW, Yang M, Taghipoor M, Meissner R, Brugger J, Renaud P (2015) Impedance sensing of DNA immobilization and hybridization by microfabricated alumina nanopore membranes. Sens Actuators B Chem 216:105–112

    CAS  Google Scholar 

  • Xing L, Ma Z (2016) A glassy carbon electrode modified with a nanocomposite consisting of MoS2 and reduced graphene oxide for electrochemical simultaneous determination of ascorbic acid, dopamine, and uric acid. Microchim Acta 183:257–263

    CAS  Google Scholar 

  • Yan M, Zang D, Ge S, Ge L, Yu J (2012) A disposable electrochemical immunosensor based on carbon screen-printed electrodes for the detection of prostate specific antigen. Biosens Bioelectron 38:355–361

    CAS  PubMed  Google Scholar 

  • Ye WW, Shi JY, Chan CY, Zhang Y, Yang M (2014) A nanoporous membrane based impedance sensing platform for DNA sensing with gold nanoparticle amplification. Sens Actuators B Chem 193:877–882

    CAS  Google Scholar 

  • Zhang X, Ma LX, Zhang YC (2015) Electrodeposition of platinum nanosheets on C60 decorated glassy carbon electrode as a stable electrochemical biosensor for simultaneous detection of ascorbic acid, dopamine and uric acid. Electrochim Acta 177:118–127

    CAS  Google Scholar 

  • Zhao Y, Gaur G, Retterer ST, Laibinis PE, Weiss SM (2016) Flow-through porous silicon membranes for real-time label-free biosensing. Anal Chem 88:10940–10948

    CAS  PubMed  Google Scholar 

  • Zittel HE, Miller FJ (1965) A glassy-carbon electrode for voltammetry. Anal Chem 37:200–203

    CAS  Google Scholar 

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Correspondence to Divesh N. Srivastava .

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Maniya, N.H., Srivastava, D.N. (2020). Medical Diagnostics Based on Electrochemical Biosensor. In: Chandra, P., Pandey, L. (eds) Biointerface Engineering: Prospects in Medical Diagnostics and Drug Delivery . Springer, Singapore. https://doi.org/10.1007/978-981-15-4790-4_8

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